Restriction map for
pF1KB8235
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 105 GACGTC GACGT^C
Acc65I 248 GGTACC G^GTACC
AccII 20 CGCG CG^CG
AfaI 249 GTAC GT^AC
AflIII 62 ACATGT A^C[GA][CT]GT
AlwNI 385 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 609 GGGCCC GGGCC^C
Asp718I 248 GGTACC G^GTACC
BanII 609 GGGCCC G[GA]GC[CT]^C
BbeI 598 GGCGCC GGCGC^C
BcnI 276 CCGGG CC^[GC]GG
BfaI 457 CTAG C^TAG
BglII 221 AGATCT A^GATCT
BmyI 609 GGGCCC G[AGT]GC[ACT]^C
BsaBI 72 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 251 ACCGGA [AT]^CCGG[AT]
BsgI 337 GTGCAG GTGCAG(16/14)
BsiYI 365 CCTACTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 365 CCTACTCATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 609 GGGCCC G[AGT]GC[ACT]^C
BspLU11I 62 ACATGT A^CATGT
BspMI 630 GCAGGT ACCTGC(4/8)
BsrFI 449 GCCGGC [GA]^CCGG[CT]
BsrI 344 ACTGG ACTGG(1/-1)
BstUI 20 CGCG CG^CG
BstYI 221 AGATCT [GA]^GATC[CT]
Cfr10I 449 GCCGGC [GA]^CCGG[CT]
DdeI 619 CTCAG C^T[ACGT]AG
DraII 608 AGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 161 CCGTGG C^C[GA][CT]GG
EaeI 447 TGGCCG [CT]^GGCC[GA]
EarI 115 CTCTTC CTCTTC(1/4)
EcoO109I 608 AGGGCCC [GA]G^G[ACGT]CC[CT]
FauI 426 GCGGG CCCGC(4/6)
KasI 598 GGCGCC G^GCGCC
KpnI 248 GGTACC GGTAC^C
Ksp632I 115 CTCTTC CTCTTC(1/4)
MaeI 457 CTAG C^TAG
MamI 72 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 221 AGATCT [GA]^GATC[CT]
MseI 509 TTAA T^TAA
MslI 294 CATCCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 20 CGCG CG^CG
NaeI 449 GCCGGC GCC^GGC
NarI 598 GGCGCC GG^CGCC
NciI 276 CCGGG CC^[GC]GG
NgoMIV 449 GCCGGC G^CCGGC
NheI 456 GCTAGC G^CTAGC
NspI 62 ACATGT [GA]CATG^[CT]
PstI 217 CTGCAG CTGCA^G
RsaI 249 GTAC GT^AC
SfcI 217 CTGCAG C^T[GA][CT]AG
SfoI 598 GGCGCC GGC^GCC
Tru9I 509 TTAA T^TAA
XhoII 221 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AcyI 105 GACGTC G[GA]^CG[CT]C
598 GGCGCC
AlwI 45 GGATC GGATC(4/5)
209 GGATC
BsaHI 105 GACGTC G[GA]^CG[CT]C
598 GGCGCC
BsmFI 227 GGGAC GGGAC(10/14)
374 GGGAC
BsrBI 244 GAGCGG CCGCTC(-3/-3)
424 GAGCGG
DrdI 172 GACTTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
583 GACCCTGCGGTC
EcoT14I 468 CCTTGG C^C[AT][AT]GG
497 CCAAGG
HaeIII 448 GGCC GG^CC
610 GGCC
Hin1I 105 GACGTC G[GA]^CG[CT]C
598 GGCGCC
HphI 260 TCACC GGTGA(8/7)
311 TCACC
MboII 555 GAAGA GAAGA(8/7)
116 TCTTC
SfaNI 4 GCATC GCATC(5/9)
167 GCATC
StyI 468 CCTTGG C^C[AT][AT]GG
497 CCAAGG
TfiI 72 GATTC G^A[AT]TC
322 GAATC
TspRI 335 CAGTG CA[GC]TG[ACGT][ACGT]^
621 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AvaII BanI BbvI
BsaJI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII EcoRII Fnu4HI
FokI HaeII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MaeIII
MboI MnlI MspI MvaI MwoI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI TaqI TseI Tsp45I
TthHB8I