Restriction map for
pF1KB8223
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspI AvaI AvaII
AvrII BamHI BanI BbeI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BpuAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI HaeII HgaI Hin1I
HindIII HpaI KasI KpnI KspI
MaeI MaeIII MamI MfeI MluI
MroI MseI MslI MunI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tru9I Tsp45I
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccII 741 CGCG CG^CG
AflIII 500 ACGTGT A^C[GA][CT]GT
AlwI 375 GATCC GGATC(4/5)
Asp700I 528 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 796 GTGCTC G[AT]GC[AT]^C
BalI 683 TGGCCA TGG^CCA
BanII 484 GGGCTC G[GA]GC[CT]^C
BbvCI 640 GCTGAGG CCTCAGC(-5/-2)
BglI 780 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 218 CACGTA [CT]AC^GT[GA]
BsaI 435 GGTCTC GGTCTC(1/5)
BseRI 799 CTCCTC GAGGAG(10/8)
BsgI 133 GTGCAG GTGCAG(16/14)
BsiHKAI 796 GTGCTC G[AT]GC[AT]^C
BsmAI 436 GTCTC GTCTC(1/5)
BsmFI 157 GGGAC GGGAC(10/14)
BstAPI 789 GCAGTCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 741 CGCG CG^CG
BstYI 374 AGATCC [GA]^GATC[CT]
Cfr13I 584 GGGCC G^G[ACGT]CC
EarI 283 CTCTTC CTCTTC(1/4)
EcoT14I 653 CCAAGG C^C[AT][AT]GG
FauI 784 GCGGG CCCGC(4/6)
HapII 288 CCGG C^CGG
HincII 262 GTCAAC GT[CT]^[GA]AC
HindII 262 GTCAAC GT[CT]^[GA]AC
HpaII 288 CCGG C^CGG
Ksp632I 283 CTCTTC CTCTTC(1/4)
MflI 374 AGATCC [GA]^GATC[CT]
MluNI 683 TGGCCA TGG^CCA
MscI 683 TGGCCA TGG^CCA
MspI 288 CCGG C^CGG
MvnI 741 CGCG CG^CG
Sau96I 584 GGGCC G^G[ACGT]CC
SmlI 379 CTCAAG C^T[CT][GA]AG
StyI 653 CCAAGG C^C[AT][AT]GG
TfiI 122 GATTC G^A[AT]TC
Tsp509I 192 AATT ^AATT
XcmI 587 CCAGATTTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 374 AGATCC [GA]^GATC[CT]
XmnI 528 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 181 GTAC GT^AC
744 GTAC
AviII 275 TGCGCA TGC^GCA
713 TGCGCA
BspMI 776 ACCTGC ACCTGC(4/8)
444 GCAGGT
CfoI 276 GCGC GCG^C
714 GCGC
DpnII 375 GATC ^GATC
705 GATC
DsaI 126 CCGTGG C^C[GA][CT]GG
175 CCGTGG
EaeI 626 CGGCCA [CT]^GGCC[GA]
683 TGGCCA
FspI 275 TGCGCA TGC^GCA
713 TGCGCA
HhaI 276 GCGC GCG^C
714 GCGC
HinP1I 276 GCGC G^CGC
714 GCGC
MaeII 219 ACGT A^CGT
500 ACGT
MboI 375 GATC ^GATC
705 GATC
MboII 284 TCTTC GAAGA(8/7)
317 TCTTC
NdeII 375 GATC ^GATC
705 GATC
PstI 689 CTGCAG CTGCA^G
728 CTGCAG
PvuII 659 CAGCTG CAG^CTG
805 CAGCTG
RsaI 181 GTAC GT^AC
744 GTAC
Sau3AI 375 GATC ^GATC
705 GATC
SfcI 689 CTGCAG C^T[GA][CT]AG
728 CTGCAG
TaqI 213 TCGA T^CGA
707 TCGA
TthHB8I 213 TCGA T^CGA
707 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI BbvI BlpI BmyI
BpmI Bpu1102I BsaJI BsiYI BslI
Bsp1286I BsrI BssKI BstF5I BstNI
BstXI Cac8I CelII DdeI EcoRII
Fnu4HI FokI HaeIII HinfI HphI
ItaI MnlI MspA1I MvaI MwoI
PleI ScrFI SfaNI TseI TspRI