Restriction map for
pF1KB8218
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# NO CUT ENZYMES ( 162 enzymes) #
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AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DpnII DraI DraII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MboI MfeI MflI MluI MroI
MslI MunI NaeI NarI NcoI
NdeI NdeII NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI Sau3AI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
TfiI Tth111I Van91I XbaI XcmI
XhoI XhoII
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 304 GTCTAC GT^[AC][GT]AC
AcsI 424 AAATTT [GA]^AATT[CT]
AcyI 84 GACGCC G[GA]^CG[CT]C
AfaI 315 GTAC GT^AC
AlwNI 43 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 424 AAATTT [GA]^AATT[CT]
Asp700I 15 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 416 CCCGGG C^[CT]CG[GA]G
AvaII 258 GGTCC G^G[AT]CC
BalI 455 TGGCCA TGG^CCA
BanI 34 GGCACC G^G[CT][GA]CC
BbsI 292 GTCTTC GAAGAC(2/6)
BpuAI 292 GTCTTC GAAGAC(2/6)
BsaBI 94 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 84 GACGCC G[GA]^CG[CT]C
BsaI 246 GGTCTC GGTCTC(1/5)
BsoBI 416 CCCGGG C^[CT]CG[GA]G
Bsp1407I 314 TGTACA T^GTACA
BspMI 283 ACCTGC ACCTGC(4/8)
BsrGI 314 TGTACA T^GTACA
BstAPI 42 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 331 GCCTGC GC[ACGT]^[ACGT]GC
Cfr13I 258 GGTCC G^G[ACGT]CC
DraIII 360 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 455 TGGCCA [CT]^GGCC[GA]
FauI 114 CCCGC CCCGC(4/6)
Hin1I 84 GACGCC G[GA]^CG[CT]C
HinfI 406 GACTC G^A[ACGT]TC
MaeII 183 ACGT A^CGT
MaeIII 229 GTCAC ^GT[ACGT]AC
MamI 94 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 455 TGGCCA TGG^CCA
MscI 455 TGGCCA TGG^CCA
MseI 242 TTAA T^TAA
NlaIV 34 GGCACC GG[ACGT]^[ACGT]CC
PleI 406 GACTC GAGTC(4/5)
PstI 106 CTGCAG CTGCA^G
PvuII 103 CAGCTG CAG^CTG
RsaI 315 GTAC GT^AC
Sau96I 258 GGTCC G^G[ACGT]CC
SfcI 106 CTGCAG C^T[GA][CT]AG
SmaI 416 CCCGGG CCC^GGG
SmlI 352 CTCAAG C^T[CT][GA]AG
SspBI 314 TGTACA T^GTACA
Tru9I 242 TTAA T^TAA
Tsp45I 229 GTCAC ^GT[GC]AC
XmaCI 416 CCCGGG C^CCGGG
XmaI 416 CCCGGG C^CCGGG
XmnI 15 GAACAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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BmyI 33 GGGCAC G[AGT]GC[ACT]^C
413 GTGCCC
BpmI 408 CTCCAG CTGGAG(16/14)
52 CTGGAG
BsmAI 529 GAGAC GTCTC(1/5)
247 GTCTC
Bsp1286I 33 GGGCAC G[AGT]GC[ACT]^C
413 GTGCCC
BsrI 363 CCAGT ACTGG(1/-1)
410 CCAGT
CfoI 329 GCGC GCG^C
474 GCGC
EarI 262 CTCTTC CTCTTC(1/4)
508 GAAGAG
HaeIII 273 GGCC GG^CC
456 GGCC
HapII 121 CCGG C^CGG
417 CCGG
HgaI 84 GACGC GACGC(5/10)
287 GCGTC
HhaI 329 GCGC GCG^C
474 GCGC
HinP1I 329 GCGC G^CGC
474 GCGC
HpaII 121 CCGG C^CGG
417 CCGG
Ksp632I 262 CTCTTC CTCTTC(1/4)
508 GAAGAG
MspA1I 46 CCGCTG C[AC]G^C[GT]G
103 CAGCTG
MspI 121 CCGG C^CGG
417 CCGG
TaqI 65 TCGA T^CGA
92 TCGA
TspRI 364 CAGTG CA[GC]TG[ACGT][ACGT]^
411 CAGTG
TthHB8I 65 TCGA T^CGA
92 TCGA
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BsaJI BsiYI BslI BssKI BstF5I
BstNI BstUI DdeI EcoRII Fnu4HI
FokI HphI ItaI MboII MnlI
MvaI MvnI MwoI NciI ScrFI
SfaNI TseI Tsp509I