Restriction map for
pF1KB8216
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# NO CUT ENZYMES ( 178 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
BsoBI BspHI BspLU11I BsrBI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstF5I BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII CfoI
Cfr10I Cfr13I CpoI DraI DraII
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FokI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MscI MslI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI Sau96I SbfI ScaI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 331 GTCTAC GT^[AC][GT]AC
AccIII 287 TCCGGA T^CCGGA
AcsI 424 AAATTC [GA]^AATT[CT]
ApoI 424 AAATTC [GA]^AATT[CT]
AspI 66 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbsI 251 GTCTTC GAAGAC(2/6)
BcgI 562 GCAAATGTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 4 CCCGG CC^[GC]GG
BglI 151 GCCCAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 251 GTCTTC GAAGAC(2/6)
BsaWI 287 TCCGGA [AT]^CCGG[AT]
BseAI 287 TCCGGA T^CCGGA
BseRI 490 GAGGAG GAGGAG(10/8)
BsiYI 153 CCAGCTGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 153 CCAGCTGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 25 GTCCC GGGAC(10/14)
Bsp1407I 483 TGTACA T^GTACA
BspDI 175 ATCGAT AT^CGAT
BspEI 287 TCCGGA T^CCGGA
BspMI 258 GCAGGT ACCTGC(4/8)
BsrGI 483 TGTACA T^GTACA
BstAPI 39 GCAGGAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
ClaI 175 ATCGAT AT^CGAT
DpnII 381 GATC ^GATC
HphI 439 GGTGA GGTGA(8/7)
MboI 381 GATC ^GATC
MroI 287 TCCGGA T^CCGGA
NciI 4 CCCGG CC^[GC]GG
NdeII 381 GATC ^GATC
NlaIII 552 CATG CATG^
PflFI 66 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Sau3AI 381 GATC ^GATC
SfcI 32 CTACAG C^T[GA][CT]AG
SspBI 483 TGTACA T^GTACA
TspRI 90 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 66 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AciI 49 CCGC CCGC(-3/-1)
160 GCGG
AfaI 103 GTAC GT^AC
484 GTAC
BanII 339 GAGCCC G[GA]GC[CT]^C
590 GAGCCC
BfaI 275 CTAG C^TAG
409 CTAG
BmyI 339 GAGCCC G[AGT]GC[ACT]^C
590 GAGCCC
BsaJI 56 CCCAGG C^C[ACGT][ACGT]GG
131 CCCAGG
BsmAI 64 GAGAC GTCTC(1/5)
448 GTCTC
Bsp1286I 339 GAGCCC G[AGT]GC[ACT]^C
590 GAGCCC
BsrI 246 ACTGG ACTGG(1/-1)
187 CCAGT
BstNI 57 CCAGG CC^[AT]GG
132 CCAGG
Cac8I 156 GCTGGC GC[ACGT]^[ACGT]GC
399 GCAAGC
EarI 499 GAAGAG CTCTTC(1/4)
505 GAAGAG
EcoRII 57 CCAGG ^CC[AT]GG
132 CCAGG
HapII 5 CCGG C^CGG
288 CCGG
HpaII 5 CCGG C^CGG
288 CCGG
Ksp632I 499 GAAGAG CTCTTC(1/4)
505 GAAGAG
MaeI 275 CTAG C^TAG
409 CTAG
MseI 533 TTAA T^TAA
637 TTAA
MspI 5 CCGG C^CGG
288 CCGG
MvaI 57 CCAGG CC^[AT]GG
132 CCAGG
RsaI 103 GTAC GT^AC
484 GTAC
Tru9I 533 TTAA T^TAA
637 TTAA
Tsp45I 280 GTCAC ^GT[GC]AC
555 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI AlwNI BbvI BssKI DdeI
Fnu4HI HinfI ItaI MaeIII MboII
MnlI MspA1I MwoI PleI PvuII
ScrFI TaqI TfiI TseI Tsp509I
TthHB8I