Restriction map for
pF1KB8212
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstF5I BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DdeI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoRV
EcoT22I FauI FbaI FokI FseI
FspI HaeII HapII HhaI HinP1I
HincII HindII HindIII HinfI HpaI
HpaII KasI KpnI MaeI MamI
MfeI MflI MluI MroI MseI
MslI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 54 CGCG CG^CG
AcsI 262 GAATTC [GA]^AATT[CT]
AcyI 106 GGCGTC G[GA]^CG[CT]C
AfaI 145 GTAC GT^AC
AflIII 5 ACGTGT A^C[GA][CT]GT
AlwNI 161 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 262 GAATTC [GA]^AATT[CT]
AvaI 131 CCCGAG C^[CT]CG[GA]G
AvaII 360 GGACC G^G[AT]CC
BalI 29 TGGCCA TGG^CCA
BanII 325 GAGCCC G[GA]GC[CT]^C
BbsI 93 GAAGAC GAAGAC(2/6)
BmyI 325 GAGCCC G[AGT]GC[ACT]^C
BpuAI 93 GAAGAC GAAGAC(2/6)
BsaHI 106 GGCGTC G[GA]^CG[CT]C
BseRI 200 GAGGAG GAGGAG(10/8)
BsoBI 131 CCCGAG C^[CT]CG[GA]G
Bsp1286I 325 GAGCCC G[AGT]GC[ACT]^C
BspHI 11 TCATGA T^CATGA
BsrI 246 ACTGG ACTGG(1/-1)
BstEII 75 GGTCACC G^GT[ACGT]ACC
BstPI 75 GGTCACC G^GT[ACGT]ACC
BstUI 54 CGCG CG^CG
BstXI 239 CCACAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 274 GATC ^GATC
EaeI 29 TGGCCA [CT]^GGCC[GA]
EarI 252 GAAGAG CTCTTC(1/4)
EcoO65I 75 GGTCACC G^GT[ACGT]ACC
EcoRI 262 GAATTC G^AATTC
HgaI 107 GCGTC GACGC(5/10)
Hin1I 106 GGCGTC G[GA]^CG[CT]C
Ksp632I 252 GAAGAG CTCTTC(1/4)
KspI 53 CCGCGG CCGC^GG
MaeIII 76 GTCAC ^GT[ACGT]AC
MboI 274 GATC ^GATC
MluNI 29 TGGCCA TGG^CCA
MscI 29 TGGCCA TGG^CCA
MspA1I 53 CCGCGG C[AC]G^C[GT]G
MvnI 54 CGCG CG^CG
NcoI 26 CCATGG C^CATGG
NdeII 274 GATC ^GATC
PpuMI 359 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 11 TCATGA T^CATGA
RsaI 145 GTAC GT^AC
SacII 53 CCGCGG CCGC^GG
Sau3AI 274 GATC ^GATC
StuI 185 AGGCCT AGG^CCT
Tsp45I 76 GTCAC ^GT[GC]AC
Tsp509I 263 AATT ^AATT
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# TWO CUT ENZYMES ( 15 enzymes) #
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AluI 204 AGCT AG^CT
303 AGCT
BssKI 297 CCAGG ^CC[ACGT]GG
369 CCAGG
BstNI 297 CCAGG CC^[AT]GG
369 CCAGG
DraII 17 AGGGCCT [GA]G^G[ACGT]CC[CT]
359 AGGACCC
DsaI 26 CCATGG C^C[GA][CT]GG
53 CCGCGG
EcoO109I 17 AGGGCCT [GA]G^G[ACGT]CC[CT]
359 AGGACCC
EcoRII 297 CCAGG ^CC[AT]GG
369 CCAGG
HphI 249 GGTGA GGTGA(8/7)
77 TCACC
MaeII 5 ACGT A^CGT
116 ACGT
MvaI 297 CCAGG CC^[AT]GG
369 CCAGG
MwoI 157 GCTTCAGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
295 GCCCAGGAAGC
NlaIII 12 CATG CATG^
27 CATG
NlaIV 336 GGAGCC GG[ACGT]^[ACGT]CC
360 GGACCC
ScrFI 297 CCAGG CC^[ACGT]GG
369 CCAGG
TspRI 161 CAGTG CA[GC]TG[ACGT][ACGT]^
317 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
Cfr13I EcoT14I Fnu4HI HaeIII ItaI
MboII MnlI Sau96I StyI TseI