Restriction map for
pF1KB8210
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NarI NdeI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AseI 222 ATTAAT AT^TAAT
AsnI 222 ATTAAT AT^TAAT
BanII 578 GAGCCC G[GA]GC[CT]^C
BbvI 610 GCAGC GCAGC(8/12)
BcnI 998 CCCGG CC^[GC]GG
BfaI 283 CTAG C^TAG
BglI 254 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1115 AGATCT A^GATCT
BmyI 578 GAGCCC G[AGT]GC[ACT]^C
BsaBI 774 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 163 CTGCAC GTGCAG(16/14)
BsmFI 528 GGGAC GGGAC(10/14)
BsmI 1053 GAATGC GAATGC(1/-1)
Bsp1286I 578 GAGCCC G[AGT]GC[ACT]^C
BspHI 885 TCATGA T^CATGA
BsrFI 687 GCCGGC [GA]^CCGG[CT]
BstAPI 691 GCAAACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 644 CCAGAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 613 GCGC GCG^C
Cfr10I 687 GCCGGC [GA]^CCGG[CT]
DraI 138 TTTAAA TTT^AAA
DraII 528 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 479 CCATGG C^C[GA][CT]GG
EaeI 641 CGGCCA [CT]^GGCC[GA]
EcoNI 1089 CCTATTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 528 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 847 GAATTC G^AATTC
EcoT14I 479 CCATGG C^C[AT][AT]GG
EcoT22I 109 ATGCAT ATGCA^T
HhaI 613 GCGC GCG^C
HinP1I 613 GCGC G^CGC
MaeI 283 CTAG C^TAG
MaeII 66 ACGT A^CGT
MamI 774 GATGGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 256 CAGCTG C[AC]G^C[GT]G
NaeI 687 GCCGGC GCC^GGC
NciI 998 CCCGG CC^[GC]GG
NcoI 479 CCATGG C^CATGG
NgoMIV 687 GCCGGC G^CCGGC
NheI 282 GCTAGC G^CTAGC
NlaIV 528 GGGACC GG[ACGT]^[ACGT]CC
NsiI 109 ATGCAT ATGCA^T
PpuMI 528 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 222 ATTAAT AT^TAAT
PvuII 256 CAGCTG CAG^CTG
RcaI 885 TCATGA T^CATGA
StyI 479 CCATGG C^C[AT][AT]GG
TseI 610 GCAGC G^C[AT]GC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 144 GCGG CCGC(-3/-1)
640 GCGG
AfaI 716 GTAC GT^AC
1067 GTAC
BpmI 317 CTGGAG CTGGAG(16/14)
542 CTGGAG
BsrDI 27 CATTGC GCAATG(2/0)
925 GCAATG
BstF5I 93 GGATG GGATG(2/0)
1083 GGATG
BstNI 88 CCAGG CC^[AT]GG
1012 CCTGG
EcoRII 88 CCAGG ^CC[AT]GG
1012 CCTGG
Fnu4HI 610 GCAGC GC^[ACGT]GC
640 GCGGC
FokI 93 GGATG GGATG(9/13)
1083 GGATG
HapII 688 CCGG C^CGG
999 CCGG
HpaII 688 CCGG C^CGG
999 CCGG
HphI 894 GGTGA GGTGA(8/7)
417 TCACC
ItaI 610 GCAGC GC^[ACGT]GC
640 GCGGC
MaeIII 874 GTGAC ^GT[ACGT]AC
895 GTGAC
MspI 688 CCGG C^CGG
999 CCGG
MvaI 88 CCAGG CC^[AT]GG
1012 CCTGG
PflMI 88 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1078 CCAGAGGATGG
RsaI 716 GTAC GT^AC
1067 GTAC
Tsp45I 874 GTGAC ^GT[GC]AC
895 GTGAC
Van91I 88 CCAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1078 CCAGAGGATGG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BsaJI BsiYI BslI BspMI BsrI
BssKI BstYI Cac8I Cfr13I DdeI
DpnII HaeIII HinfI MboI MboII
MflI MnlI MseI MwoI NdeII
NlaIII Sau3AI Sau96I ScrFI SfaNI
TaqI TfiI Tru9I Tsp509I TspRI
TthHB8I XhoII