Restriction map for
pF1KB8209
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AvrII BamHI BbeI BbrPI BciVI
BclI BcnI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BseAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DrdI DsaI Eam1105I EarI
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MamI MfeI MroI
MseI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tru9I Tsp509I
TspRI Tth111I TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 371 GTCTAC GT^[AC][GT]AC
AflIII 219 ACGCGT A^C[GA][CT]GT
AgeI 242 ACCGGT A^CCGGT
AluI 276 AGCT AG^CT
AlwI 755 GGATC GGATC(4/5)
Aor51HI 506 AGCGCT AGC^GCT
ApaI 662 GGGCCC GGGCC^C
AspHI 472 GAGCAC G[AT]GC[AT]^C
AviII 497 TGCGCA TGC^GCA
BalI 749 TGGCCA TGG^CCA
BanI 391 GGTGCC G^G[CT][GA]CC
BbsI 223 GTCTTC GAAGAC(2/6)
BcgI 7 GCAGAGGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 67 CTAG C^TAG
BglI 611 GCCCAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 223 GTCTTC GAAGAC(2/6)
BsaI 541 GAGACC GGTCTC(1/5)
BsaWI 242 ACCGGT [AT]^CCGG[AT]
BsgI 178 CTGCAC GTGCAG(16/14)
BsiEI 590 CGGCCG CG[GA][CT]^CG
BsiHKAI 472 GAGCAC G[AT]GC[AT]^C
BsmBI 252 GAGACG CGTCTC(1/5)
BsrFI 242 ACCGGT [GA]^CCGG[CT]
BsrI 368 CCAGT ACTGG(1/-1)
BstAPI 271 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 322 GGATG GGATG(2/0)
BstXI 353 CCAAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 755 GGATCT [GA]^GATC[CT]
Cfr10I 242 ACCGGT [GA]^CCGG[CT]
EagI 590 CGGCCG C^GGCCG
EclXI 590 CGGCCG C^GGCCG
Eco47III 506 AGCGCT AGC^GCT
Eco52I 590 CGGCCG C^GGCCG
EcoT14I 305 CCAAGG C^C[AT][AT]GG
FokI 322 GGATG GGATG(9/13)
FspI 497 TGCGCA TGC^GCA
HphI 452 TCACC GGTGA(8/7)
MaeI 67 CTAG C^TAG
MaeIII 338 GTGAC ^GT[ACGT]AC
MflI 755 GGATCT [GA]^GATC[CT]
MluI 219 ACGCGT A^CGCGT
MluNI 749 TGGCCA TGG^CCA
MscI 749 TGGCCA TGG^CCA
MslI 381 CACGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 786 CATG CATG^
PinAI 242 ACCGGT A^CCGGT
PleI 811 GACTC GAGTC(4/5)
PvuII 275 CAGCTG CAG^CTG
ScaI 437 AGTACT AGT^ACT
SexAI 454 ACCTGGT A^CC[AT]GGT
SfaNI 70 GATGC GCATC(5/9)
StyI 305 CCAAGG C^C[AT][AT]GG
Tsp45I 338 GTGAC ^GT[GC]AC
XcmI 613 CCAGATGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 755 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AfaI 144 GTAC GT^AC
438 GTAC
AlwNI 272 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
652 CAGGAACTG
BbvCI 465 CCTCAGC CCTCAGC(-5/-2)
235 GCTGAGG
BseRI 76 GAGGAG GAGGAG(10/8)
79 GAGGAG
BsmAI 252 GAGAC GTCTC(1/5)
541 GAGAC
BsrBI 729 CCGCTC CCGCTC(-3/-3)
515 GAGCGG
DpnII 421 GATC ^GATC
756 GATC
MaeII 182 ACGT A^CGT
690 ACGT
MboI 421 GATC ^GATC
756 GATC
MspA1I 43 CCGCTG C[AC]G^C[GT]G
275 CAGCTG
NdeII 421 GATC ^GATC
756 GATC
PpuMI 599 GGGACCC [GA]G^G[AT]CC[CT]
792 GGGACCT
RsaI 144 GTAC GT^AC
438 GTAC
Sau3AI 421 GATC ^GATC
756 GATC
TfiI 13 GAATC G^A[AT]TC
91 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AccII AciI AvaII BanII BbvI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssKI BstNI BstUI Cac8I
CfoI Cfr13I DdeI DraII EaeI
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI MboII MnlI
MspI MvaI MvnI MwoI NlaIV
Sau96I ScrFI SmlI TseI