Restriction map for
pF1KB8205
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BalI BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI Ksp632I KspI MaeII
MamI MboII MfeI MluI MluNI
MroI MscI MseI MunI NaeI
NcoI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfuI
SmaI SmlI SnaBI SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccII 210 CGCG CG^CG
AocI 1000 CCTGAGG CC^T[ACGT]AGG
AvaI 355 CTCGGG C^[CT]CG[GA]G
BglI 247 GCCAGCCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 913 GAGACC GGTCTC(1/5)
BsaWI 590 ACCGGA [AT]^CCGG[AT]
BsmAI 913 GAGAC GTCTC(1/5)
BsmFI 634 GTCCC GGGAC(10/14)
BsmI 264 GAATGC GAATGC(1/-1)
BsoBI 355 CTCGGG C^[CT]CG[GA]G
BspMI 863 ACCTGC ACCTGC(4/8)
BsrDI 602 GCAATG GCAATG(2/0)
BsrFI 6 GCCGGT [GA]^CCGG[CT]
BstEII 571 GGTGACC G^GT[ACGT]ACC
BstPI 571 GGTGACC G^GT[ACGT]ACC
BstUI 210 CGCG CG^CG
BstXI 464 CCAAAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1000 CCTGAGG CC^T[ACGT]AGG
Cfr10I 6 GCCGGT [GA]^CCGG[CT]
DsaI 513 CCACGG C^C[GA][CT]GG
Eco81I 1000 CCTGAGG CC^T[ACGT]AGG
EcoO65I 571 GGTGACC G^GT[ACGT]ACC
HgaI 98 GCGTC GACGC(5/10)
MslI 438 CACAGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 210 CGCG CG^CG
NheI 360 GCTAGC G^CTAGC
NspI 809 GCATGC [GA]CATG^[CT]
SbfI 986 CCTGCAGG CCTGCA^GG
SgrAI 5 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 231 ACTAGT A^CTAGT
SphI 809 GCATGC GCATG^C
Sse8387I 986 CCTGCAGG CCTGCA^GG
StuI 612 AGGCCT AGG^CCT
TaqI 716 TCGA T^CGA
TthHB8I 716 TCGA T^CGA
XcmI 464 CCAAAAGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 494 GGTACC G^GTACC
860 GGTACC
AlwNI 835 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
995 CAGCACCTG
ApaI 300 GGGCCC GGGCC^C
746 GGGCCC
Asp718I 494 GGTACC G^GTACC
860 GGTACC
AspHI 775 GAGCAC G[AT]GC[AT]^C
844 GTGCTC
BamHI 564 GGATCC G^GATCC
593 GGATCC
BbeI 3 GGCGCC GGCGC^C
509 GGCGCC
BfaI 232 CTAG C^TAG
361 CTAG
BsiHKAI 775 GAGCAC G[AT]GC[AT]^C
844 GTGCTC
EcoNI 183 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
828 CCTACTGCAGG
HinfI 381 GAGTC G^A[ACGT]TC
694 GACTC
KasI 3 GGCGCC G^GCGCC
509 GGCGCC
KpnI 494 GGTACC GGTAC^C
860 GGTACC
MaeI 232 CTAG C^TAG
361 CTAG
MspA1I 203 CAGCGG C[AC]G^C[GT]G
304 CCGCTG
NarI 3 GGCGCC GG^CGCC
509 GGCGCC
PleI 694 GACTC GAGTC(4/5)
381 GAGTC
PpuMI 293 GGGTCCT [GA]G^G[AT]CC[CT]
632 GGGTCCC
SfaNI 901 GATGC GCATC(5/9)
41 GCATC
SfoI 3 GGCGCC GGC^GCC
509 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII HphI ItaI MaeIII
MboI MflI MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfcI StyI TseI Tsp45I TspRI
XhoII