Restriction map for
pF1KB8195
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstXI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MseI MspA1I MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PstI
PvuI PvuII RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tru9I Tsp509I Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AccIII 896 TCCGGA T^CCGGA
AcyI 906 GACGCC G[GA]^CG[CT]C
AlwI 894 GATCC GGATC(4/5)
AocI 222 CCTGAGG CC^T[ACGT]AGG
AspHI 355 GAGCTC G[AT]GC[AT]^C
BalI 809 TGGCCA TGG^CCA
BanI 784 GGCACC G^G[CT][GA]CC
BbrPI 523 CACGTG CAC^GTG
BbsI 142 GAAGAC GAAGAC(2/6)
BpuAI 142 GAAGAC GAAGAC(2/6)
BsaAI 523 CACGTG [CT]AC^GT[GA]
BsaHI 906 GACGCC G[GA]^CG[CT]C
BsaWI 896 TCCGGA [AT]^CCGG[AT]
BseAI 896 TCCGGA T^CCGGA
BsgI 433 GTGCAG GTGCAG(16/14)
BsiHKAI 355 GAGCTC G[AT]GC[AT]^C
BspEI 896 TCCGGA T^CCGGA
BspHI 689 TCATGA T^CATGA
BsrBI 445 GAGCGG CCGCTC(-3/-3)
BsrFI 664 GCCGGC [GA]^CCGG[CT]
BstBI 702 TTCGAA TT^CGAA
BstYI 893 AGATCC [GA]^GATC[CT]
Bsu36I 222 CCTGAGG CC^T[ACGT]AGG
Cfr10I 664 GCCGGC [GA]^CCGG[CT]
DpnII 894 GATC ^GATC
DrdI 579 GACATACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 809 TGGCCA [CT]^GGCC[GA]
Eco81I 222 CCTGAGG CC^T[ACGT]AGG
EcoT14I 770 CCTTGG C^C[AT][AT]GG
HgaI 906 GACGC GACGC(5/10)
Hin1I 906 GACGCC G[GA]^CG[CT]C
HphI 132 GGTGA GGTGA(8/7)
MaeII 524 ACGT A^CGT
MboI 894 GATC ^GATC
MboII 142 GAAGA GAAGA(8/7)
MflI 893 AGATCC [GA]^GATC[CT]
MluNI 809 TGGCCA TGG^CCA
MroI 896 TCCGGA T^CCGGA
MscI 809 TGGCCA TGG^CCA
MslI 690 CATGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 664 GCCGGC GCC^GGC
NdeII 894 GATC ^GATC
NgoMIV 664 GCCGGC G^CCGGC
NspV 702 TTCGAA TT^CGAA
PflMI 108 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 523 CACGTG CAC^GTG
PmlI 523 CACGTG CAC^GTG
PpuMI 218 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 689 TCATGA T^CATGA
SacI 355 GAGCTC GAGCT^C
Sau3AI 894 GATC ^GATC
SfuI 702 TTCGAA TT^CGAA
SmlI 595 CTCAAG C^T[CT][GA]AG
StuI 479 AGGCCT AGG^CCT
StyI 770 CCTTGG C^C[AT][AT]GG
TfiI 726 GATTC G^A[AT]TC
Van91I 108 CCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 893 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AvaII 219 GGACC G^G[AT]CC
564 GGACC
BanII 228 GAGCCC G[GA]GC[CT]^C
355 GAGCTC
BcnI 465 CCCGG CC^[GC]GG
781 CCCGG
BmyI 228 GAGCCC G[AGT]GC[ACT]^C
355 GAGCTC
BseRI 225 GAGGAG GAGGAG(10/8)
253 GAGGAG
BsmAI 118 GAGAC GTCTC(1/5)
577 GAGAC
Bsp1286I 228 GAGCCC G[AGT]GC[ACT]^C
355 GAGCTC
BsrI 36 CCAGT ACTGG(1/-1)
108 CCAGT
EcoNI 482 CCTACGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
816 CCTCATCCAGG
MaeIII 133 GTGAC ^GT[ACGT]AC
149 GTGAC
NciI 465 CCCGG CC^[GC]GG
781 CCCGG
SfcI 402 CTACAG C^T[GA][CT]AG
471 CTACAG
Tsp45I 133 GTGAC ^GT[GC]AC
149 GTGAC
TspRI 109 CAGTG CA[GC]TG[ACGT][ACGT]^
147 CAGTG
XcmI 104 CCATCCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
635 CCACGGGAGGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AluI AlwNI BbvI
BpmI BsaJI BsiYI BslI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DraII DsaI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI MnlI MspI MvaI
MvnI MwoI NlaIII NlaIV PleI
Sau96I ScrFI SfaNI TaqI TseI
TthHB8I