Restriction map for
pF1KB8194
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BmyI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I Bsp1407I BspEI BspHI BsrBI
BsrFI BsrGI BssHII BstAPI BstF5I
BstUI BstXI Bsu36I CfoI Cfr10I
CpoI DraII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRI EcoT22I FbaI
FokI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 328 GTATAC GT^[AC][GT]AC
AclI 516 AACGTT AA^CGTT
AflIII 701 ACATGT A^C[GA][CT]GT
AvaI 1079 CTCGAG C^[CT]CG[GA]G
BalI 818 TGGCCA TGG^CCA
BanI 1573 GGCACC G^G[CT][GA]CC
BbsI 1318 GAAGAC GAAGAC(2/6)
BglII 21 AGATCT A^GATCT
BlpI 34 GCTGAGC GC^T[ACGT]AGC
BpmI 727 CTGGAG CTGGAG(16/14)
Bpu1102I 34 GCTGAGC GC^T[ACGT]AGC
BpuAI 1318 GAAGAC GAAGAC(2/6)
BsaWI 829 TCCGGT [AT]^CCGG[AT]
BseRI 194 CTCCTC GAGGAG(10/8)
BsoBI 1079 CTCGAG C^[CT]CG[GA]G
BspDI 106 ATCGAT AT^CGAT
BspLU11I 701 ACATGT A^CATGT
BspMI 863 ACCTGC ACCTGC(4/8)
BsrDI 180 CATTGC GCAATG(2/0)
Bst1107I 328 GTATAC GTA^TAC
BstEII 1141 GGTAACC G^GT[ACGT]ACC
BstPI 1141 GGTAACC G^GT[ACGT]ACC
BstZ17I 328 GTATAC GTA^TAC
CelII 34 GCTGAGC GC^T[ACGT]AGC
ClaI 106 ATCGAT AT^CGAT
DraI 835 TTTAAA TTT^AAA
DsaI 1072 CCATGG C^C[GA][CT]GG
EcoO65I 1141 GGTAACC G^GT[ACGT]ACC
EcoT14I 1072 CCATGG C^C[AT][AT]GG
FauI 164 CCCGC CCCGC(4/6)
HincII 705 GTTGAC GT[CT]^[GA]AC
HindII 705 GTTGAC GT[CT]^[GA]AC
MluNI 818 TGGCCA TGG^CCA
MscI 818 TGGCCA TGG^CCA
MspA1I 134 CAGCTG C[AC]G^C[GT]G
NcoI 1072 CCATGG C^CATGG
NdeI 850 CATATG CA^TATG
PaeR7I 1079 CTCGAG C^TCGAG
Psp1406I 516 AACGTT AA^CGTT
PvuII 134 CAGCTG CAG^CTG
SmlI 1079 CTCGAG C^T[CT][GA]AG
SphI 1493 GCATGC GCATG^C
SspI 598 AATATT AAT^ATT
StyI 1072 CCATGG C^C[AT][AT]GG
XcmI 372 CCAGTCTTACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1079 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 165 CCGC CCGC(-3/-1)
12 GCGG
BamHI 1052 GGATCC G^GATCC
1461 GGATCC
BcnI 977 CCGGG CC^[GC]GG
1458 CCGGG
BsgI 717 CTGCAC GTGCAG(16/14)
739 GTGCAG
BsrI 382 ACTGG ACTGG(1/-1)
372 CCAGT
BssSI 88 CACGAG CACGAG(-5/-1)
1063 CTCGTG
BstBI 557 TTCGAA TT^CGAA
1387 TTCGAA
Cac8I 1155 GCTGGC GC[ACGT]^[ACGT]GC
1493 GCATGC
DraIII 636 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
784 CACACTGTG
EaeI 818 TGGCCA [CT]^GGCC[GA]
1246 CGGCCA
EcoRV 175 GATATC GAT^ATC
1560 GATATC
MaeII 517 ACGT A^CGT
872 ACGT
MslI 691 CATTTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1426 CATGTGTATG
NciI 977 CCGGG CC^[GC]GG
1458 CCGGG
NspV 557 TTCGAA TT^CGAA
1387 TTCGAA
PleI 1077 GACTC GAGTC(4/5)
805 GAGTC
SexAI 1031 ACCTGGT A^CC[AT]GGT
1253 ACCAGGT
SfuI 557 TTCGAA TT^CGAA
1387 TTCGAA
Tsp45I 698 GTGAC ^GT[GC]AC
1028 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BsaJI BsiYI
BslI BsmAI BsmFI BssKI BstNI
BstYI Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII