Restriction map for
pF1KB8192
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# NO CUT ENZYMES ( 169 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FokI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HpaI KasI KpnI KspI MaeI
MaeII MamI MfeI MflI MluI
MroI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AflIII 539 ACATGT A^C[GA][CT]GT
AlwI 323 GGATC GGATC(4/5)
AlwNI 52 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 347 GTGCTC G[AT]GC[AT]^C
BalI 459 TGGCCA TGG^CCA
BciVI 519 GTATCC GTATCC(6/5)
BcnI 377 CCCGG CC^[GC]GG
BmyI 347 GTGCTC G[AGT]GC[ACT]^C
BsaI 381 GAGACC GGTCTC(1/5)
BsiHKAI 347 GTGCTC G[AT]GC[AT]^C
BsmFI 371 GGGAC GGGAC(10/14)
Bsp1286I 347 GTGCTC G[AGT]GC[ACT]^C
BspHI 132 TCATGA T^CATGA
BspLU11I 539 ACATGT A^CATGT
BstAPI 331 GCAAGTAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 352 CCACTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 3 GCTGGC GC[ACGT]^[ACGT]GC
DpnII 324 GATC ^GATC
DsaI 523 CCATGG C^C[GA][CT]GG
EaeI 459 TGGCCA [CT]^GGCC[GA]
EarI 165 GAAGAG CTCTTC(1/4)
EcoT14I 523 CCATGG C^C[AT][AT]GG
HaeIII 460 GGCC GG^CC
HapII 378 CCGG C^CGG
HincII 636 GTCAAC GT[CT]^[GA]AC
HindII 636 GTCAAC GT[CT]^[GA]AC
HpaII 378 CCGG C^CGG
HphI 72 TCACC GGTGA(8/7)
Ksp632I 165 GAAGAG CTCTTC(1/4)
MboI 324 GATC ^GATC
MluNI 459 TGGCCA TGG^CCA
MscI 459 TGGCCA TGG^CCA
MspA1I 230 CAGCTG C[AC]G^C[GT]G
MspI 378 CCGG C^CGG
NciI 377 CCCGG CC^[GC]GG
NcoI 523 CCATGG C^CATGG
NdeII 324 GATC ^GATC
NspI 539 ACATGT [GA]CATG^[CT]
PflMI 51 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 230 CAGCTG CAG^CTG
RcaI 132 TCATGA T^CATGA
Sau3AI 324 GATC ^GATC
SexAI 50 ACCAGGT A^CC[AT]GGT
SfaNI 475 GATGC GCATC(5/9)
StyI 523 CCATGG C^C[AT][AT]GG
TaqI 544 TCGA T^CGA
Tsp45I 575 GTGAC ^GT[GC]AC
TthHB8I 544 TCGA T^CGA
Van91I 51 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 514 CCACAGTATCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 85 GAATTT [GA]^AATT[CT]
125 AAATTC
ApoI 85 GAATTT [GA]^AATT[CT]
125 AAATTC
AvaII 54 GGTCC G^G[AT]CC
372 GGACC
BbvI 186 GCAGC GCAGC(8/12)
484 GCAGC
BpmI 38 CTGGAG CTGGAG(16/14)
58 CTGGAG
BseRI 291 CTCCTC GAGGAG(10/8)
645 CTCCTC
BsmAI 170 GAGAC GTCTC(1/5)
381 GAGAC
BsrI 222 ACTGG ACTGG(1/-1)
420 ACTGG
Cfr13I 54 GGTCC G^G[ACGT]CC
372 GGACC
DraII 53 AGGTCCT [GA]G^G[ACGT]CC[CT]
371 GGGACCC
EcoO109I 53 AGGTCCT [GA]G^G[ACGT]CC[CT]
371 GGGACCC
Fnu4HI 186 GCAGC GC^[ACGT]GC
484 GCAGC
HinfI 643 GACTC G^A[ACGT]TC
664 GAGTC
ItaI 186 GCAGC GC^[ACGT]GC
484 GCAGC
PleI 643 GACTC GAGTC(4/5)
664 GAGTC
PpuMI 53 AGGTCCT [GA]G^G[AT]CC[CT]
371 GGGACCC
Sau96I 54 GGTCC G^G[ACGT]CC
372 GGACC
SmlI 256 CTCAAG C^T[CT][GA]AG
648 CTCAAG
TseI 186 GCAGC G^C[AT]GC
484 GCAGC
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# 3 OR MORE CUT ENZYMES ( 20 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BssKI
BstNI EcoRII MaeIII MboII MnlI
MseI MslI MvaI MwoI NlaIII
NlaIV ScrFI Tru9I Tsp509I TspRI