##################################
# NO CUT ENZYMES (  208 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AviII       AvrII       BalI        
BamHI       BanI        BanII       BbeI        BbrPI       
BbsI        BbvCI       BcgI        BciVI       BclI        
BcnI        BglI        BglII       BlnI        BlpI        
BmyI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrFI       BsrGI       
BsrI        BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstF5I      BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FauI        FbaI        
FokI        FseI        FspI        HaeII       HaeIII      
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HinfI       HpaI        HpaII       
HphI        KasI        KpnI        KspI        MaeII       
MaeIII      MfeI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        StyI        
SwaI        TfiI        Tsp45I      Tsp509I     TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AccI           40  GTAGAC               GT^[AC][GT]AC
AfaI           59  GTAC                 GT^AC
AflII         153  CTTAAG               C^TTAAG
AvaII         189  GGACC                G^G[AT]CC
BfrI          153  CTTAAG               C^TTAAG
BpmI          287  CTCCAG               CTGGAG(16/14)
BsaBI          97  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         362  GAGGAG               GAGGAG(10/8)
BsrDI         367  GCAATG               GCAATG(2/0)
Cfr13I        189  GGACC                G^G[ACGT]CC
EarI          144  CTCTTC               CTCTTC(1/4)
Ksp632I       144  CTCTTC               CTCTTC(1/4)
MamI           97  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          154  TTAA                 T^TAA
NdeI          265  CATATG               CA^TATG
RsaI           59  GTAC                 GT^AC
Sau96I        189  GGACC                G^G[ACGT]CC
ScaI           58  AGTACT               AGT^ACT
SexAI         176  ACCTGGT              A^CC[AT]GGT
SmlI          153  CTTAAG               C^T[CT][GA]AG
SspI          412  AATATT               AAT^ATT
TaqI          360  TCGA                 T^CGA
Tru9I         154  TTAA                 T^TAA
TthHB8I       360  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BfaI           26  CTAG                 C^TAG
              121  CTAG                
BssKI          45  CCAGG                ^CC[ACGT]GG
              177  CCTGG               
BstNI          45  CCAGG                CC^[AT]GG
              177  CCTGG               
BstYI         102  AGATCC               [GA]^GATC[CT]
              283  GGATCT              
EcoRII         45  CCAGG                ^CC[AT]GG
              177  CCTGG               
HindIII       312  AAGCTT               A^AGCTT
              379  AAGCTT              
MaeI           26  CTAG                 C^TAG
              121  CTAG                
MflI          102  AGATCC               [GA]^GATC[CT]
              283  GGATCT              
MvaI           45  CCAGG                CC^[AT]GG
              177  CCTGG               
PstI          115  CTGCAG               CTGCA^G
              197  CTGCAG              
ScrFI          45  CCAGG                CC^[ACGT]GG
              177  CCTGG               
SfcI          115  CTGCAG               C^T[GA][CT]AG
              197  CTGCAG              
XhoII         102  AGATCC               [GA]^GATC[CT]
              283  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AluI        AlwI        BbvI        DdeI        DpnII       
Fnu4HI      ItaI        MboI        MboII       MnlI        
NdeII       Sau3AI      TseI