Restriction map for
pF1KB8175
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# NO CUT ENZYMES ( 138 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaI BseAI BsgI BsiEI BsiWI
BsmBI BspDI BspEI BspLU11I BsrBI
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FseI FspI
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacII SalI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tth111I Van91I XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 58 GACGTC GACGT^C
AlwI 1479 GGATC GGATC(4/5)
AocI 168 CCTGAGG CC^T[ACGT]AGG
ApaI 1252 GGGCCC GGGCC^C
AspHI 211 GAGCTC G[AT]GC[AT]^C
BanI 925 GGCACC G^G[CT][GA]CC
BclI 117 TGATCA T^GATCA
BcnI 99 CCGGG CC^[GC]GG
BsaBI 785 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 284 ACCGGA [AT]^CCGG[AT]
BsiHKAI 211 GAGCTC G[AT]GC[AT]^C
Bsp1407I 1032 TGTACA T^GTACA
BspMI 1145 ACCTGC ACCTGC(4/8)
BsrDI 957 CATTGC GCAATG(2/0)
BsrGI 1032 TGTACA T^GTACA
BstXI 1150 CCACTTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 168 CCTGAGG CC^T[ACGT]AGG
EaeI 685 TGGCCG [CT]^GGCC[GA]
Eco81I 168 CCTGAGG CC^T[ACGT]AGG
FauI 824 GCGGG CCCGC(4/6)
FbaI 117 TGATCA T^GATCA
HaeII 26 GGCGCT [GA]GCGC^[CT]
HgaI 1287 GCGTC GACGC(5/10)
HindIII 1192 AAGCTT A^AGCTT
MaeII 59 ACGT A^CGT
MamI 785 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 449 TTAA T^TAA
MspA1I 22 CAGCGG C[AC]G^C[GT]G
NciI 99 CCGGG CC^[GC]GG
PaeR7I 347 CTCGAG C^TCGAG
SacI 211 GAGCTC GAGCT^C
SspBI 1032 TGTACA T^GTACA
Tru9I 449 TTAA T^TAA
XbaI 966 TCTAGA T^CTAGA
XhoI 347 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AcyI 58 GACGTC G[GA]^CG[CT]C
1286 GGCGTC
BbsI 1438 GAAGAC GAAGAC(2/6)
1467 GAAGAC
BglII 302 AGATCT A^GATCT
977 AGATCT
BpuAI 1438 GAAGAC GAAGAC(2/6)
1467 GAAGAC
BsaHI 58 GACGTC G[GA]^CG[CT]C
1286 GGCGTC
BsmI 534 GAATGC GAATGC(1/-1)
742 GCATTC
BspHI 33 TCATGA T^CATGA
1023 TCATGA
BsrI 12 ACTGG ACTGG(1/-1)
1181 ACTGG
CfoI 27 GCGC GCG^C
1038 GCGC
EcoRI 247 GAATTC G^AATTC
277 GAATTC
HapII 99 CCGG C^CGG
285 CCGG
HhaI 27 GCGC GCG^C
1038 GCGC
Hin1I 58 GACGTC G[GA]^CG[CT]C
1286 GGCGTC
HinP1I 27 GCGC G^CGC
1038 GCGC
HpaII 99 CCGG C^CGG
285 CCGG
HphI 80 TCACC GGTGA(8/7)
120 TCACC
MspI 99 CCGG C^CGG
285 CCGG
RcaI 33 TCATGA T^CATGA
1023 TCATGA
TaqI 348 TCGA T^CGA
419 TCGA
Tsp45I 882 GTCAC ^GT[GC]AC
1345 GTGAC
TthHB8I 348 TCGA T^CGA
419 TCGA
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# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaI AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII Fnu4HI FokI HaeIII
HinfI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MslI
MvaI MwoI NdeII NlaIII NlaIV
PleI PpuMI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SmlI TfiI
TseI Tsp509I TspRI XhoII