Restriction map for
pF1KB8171
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflIII AgeI AhdI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BanII BbrPI BbvCI BcgI BclI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRV FauI FbaI FseI FspI
HpaI KpnI Ksp632I KspI MaeII
MamI MfeI MflI MluI MroI
MunI MvnI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XbaI XcmI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AcyI 1090 GGCGCC G[GA]^CG[CT]C
AflII 606 CTTAAG C^TTAAG
AlwI 1320 GATCC GGATC(4/5)
AspI 1501 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1358 CCCGGG C^[CT]CG[GA]G
BalI 354 TGGCCA TGG^CCA
BanI 1090 GGCGCC G^G[CT][GA]CC
BbeI 1090 GGCGCC GGCGC^C
BciVI 1097 GGATAC GTATCC(6/5)
BfaI 1663 CTAG C^TAG
BfrI 606 CTTAAG C^TTAAG
BsaHI 1090 GGCGCC G[GA]^CG[CT]C
BsoBI 1358 CCCGGG C^[CT]CG[GA]G
BspHI 1122 TCATGA T^CATGA
BspMI 205 ACCTGC ACCTGC(4/8)
BsrFI 671 GCCGGC [GA]^CCGG[CT]
BstEII 1472 GGTCACC G^GT[ACGT]ACC
BstPI 1472 GGTCACC G^GT[ACGT]ACC
BstXI 35 CCAACACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 671 GCCGGC [GA]^CCGG[CT]
DpnII 1320 GATC ^GATC
DraII 956 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 133 GACCCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 956 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1472 GGTCACC G^GT[ACGT]ACC
EcoT22I 877 ATGCAT ATGCA^T
HgaI 1270 GCGTC GACGC(5/10)
Hin1I 1090 GGCGCC G[GA]^CG[CT]C
HincII 181 GTTGAC GT[CT]^[GA]AC
HindII 181 GTTGAC GT[CT]^[GA]AC
HindIII 1019 AAGCTT A^AGCTT
KasI 1090 GGCGCC G^GCGCC
MaeI 1663 CTAG C^TAG
MboI 1320 GATC ^GATC
MluNI 354 TGGCCA TGG^CCA
MscI 354 TGGCCA TGG^CCA
NaeI 671 GCCGGC GCC^GGC
NarI 1090 GGCGCC GG^CGCC
NdeII 1320 GATC ^GATC
NgoMIV 671 GCCGGC G^CCGGC
NsiI 877 ATGCAT ATGCA^T
NspI 1436 ACATGC [GA]CATG^[CT]
PflFI 1501 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1587 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 956 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 1122 TCATGA T^CATGA
Sau3AI 1320 GATC ^GATC
SfcI 932 CTATAG C^T[GA][CT]AG
SfoI 1090 GGCGCC GGC^GCC
SmaI 1358 CCCGGG CCC^GGG
Tth111I 1501 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1587 CCAAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1358 CCCGGG C^CCGGG
XmaI 1358 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Alw44I 345 GTGCAC G^TGCAC
577 GTGCAC
ApaLI 345 GTGCAC G^TGCAC
577 GTGCAC
Asp700I 538 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
621 GAAATCATTC
AspHI 345 GTGCAC G[AT]GC[AT]^C
577 GTGCAC
AvaII 957 GGACC G^G[AT]CC
1225 GGTCC
BbsI 1147 GAAGAC GAAGAC(2/6)
1202 GTCTTC
BcnI 1358 CCCGG CC^[GC]GG
1359 CCGGG
BpmI 1103 CTCCAG CTGGAG(16/14)
1544 CTCCAG
BpuAI 1147 GAAGAC GAAGAC(2/6)
1202 GTCTTC
BsaI 659 GAGACC GGTCTC(1/5)
1116 GGTCTC
BseRI 256 GAGGAG GAGGAG(10/8)
758 GAGGAG
BsiHKAI 345 GTGCAC G[AT]GC[AT]^C
577 GTGCAC
BsmI 478 GCATTC GAATGC(1/-1)
879 GCATTC
CfoI 267 GCGC GCG^C
1091 GCGC
Cfr13I 957 GGACC G^G[ACGT]CC
1225 GGTCC
EaeI 354 TGGCCA [CT]^GGCC[GA]
1349 TGGCCG
EcoRI 730 GAATTC G^AATTC
1169 GAATTC
HaeII 266 AGCGCC [GA]GCGC^[CT]
1090 GGCGCC
HapII 672 CCGG C^CGG
1359 CCGG
HhaI 267 GCGC GCG^C
1091 GCGC
HinP1I 267 GCGC G^CGC
1091 GCGC
HpaII 672 CCGG C^CGG
1359 CCGG
MspI 672 CCGG C^CGG
1359 CCGG
NciI 1358 CCCGG CC^[GC]GG
1359 CCGGG
PleI 700 GACTC GAGTC(4/5)
25 GAGTC
Sau96I 957 GGACC G^G[ACGT]CC
1225 GGTCC
SmlI 148 CTCAAG C^T[CT][GA]AG
606 CTTAAG
TaqI 739 TCGA T^CGA
1497 TCGA
TthHB8I 739 TCGA T^CGA
1497 TCGA
XmnI 538 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
621 GAAATCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI BbvI BmyI BsaJI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstAPI BstF5I
BstNI Cac8I DdeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeIII MboII MnlI MseI
MslI MspA1I MvaI MwoI NlaIII
NlaIV PvuII RsaI ScrFI SfaNI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI