Restriction map for
pF1KB8163
##################################
# NO CUT ENZYMES ( 187 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvrII BalI BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BsgI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DdeI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FokI
FseI HaeII HgaI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeIII MamI MflI
MluI MluNI MroI MscI MseI
MslI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp45I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XhoII
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AflIII 218 ACATGT A^C[GA][CT]GT
Asp700I 330 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 83 CCCGGG C^[CT]CG[GA]G
AviII 280 TGCGCA TGC^GCA
BfaI 319 CTAG C^TAG
BpmI 193 CTCCAG CTGGAG(16/14)
BsaWI 50 ACCGGA [AT]^CCGG[AT]
BseRI 190 CTCCTC GAGGAG(10/8)
BsiYI 51 CCGGAAGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 51 CCGGAAGGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 100 GGGAC GGGAC(10/14)
BsoBI 83 CCCGGG C^[CT]CG[GA]G
BspLU11I 218 ACATGT A^CATGT
BsrBI 104 CCGCTC CCGCTC(-3/-3)
BsrDI 313 CATTGC GCAATG(2/0)
BsrI 203 ACTGG ACTGG(1/-1)
CfoI 281 GCGC GCG^C
DpnII 187 GATC ^GATC
EarI 109 CTCTTC CTCTTC(1/4)
FspI 280 TGCGCA TGC^GCA
HhaI 281 GCGC GCG^C
HinP1I 281 GCGC G^CGC
HphI 245 TCACC GGTGA(8/7)
Ksp632I 109 CTCTTC CTCTTC(1/4)
MaeI 319 CTAG C^TAG
MaeII 254 ACGT A^CGT
MboI 187 GATC ^GATC
MboII 110 TCTTC GAAGA(8/7)
MfeI 302 CAATTG C^AATTG
MspA1I 8 CAGCGG C[AC]G^C[GT]G
MunI 302 CAATTG C^AATTG
NdeII 187 GATC ^GATC
NspI 218 ACATGT [GA]CATG^[CT]
PstI 91 CTGCAG CTGCA^G
Sau3AI 187 GATC ^GATC
SfcI 91 CTGCAG C^T[GA][CT]AG
SmaI 83 CCCGGG CCC^GGG
TspRI 202 CACTG CA[GC]TG[ACGT][ACGT]^
XmaCI 83 CCCGGG C^CCGGG
XmaI 83 CCCGGG C^CCGGG
XmnI 330 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AluI 125 AGCT AG^CT
156 AGCT
AvaII 101 GGACC G^G[AT]CC
298 GGACC
BcnI 83 CCCGG CC^[GC]GG
84 CCGGG
BstNI 97 CCAGG CC^[AT]GG
195 CCAGG
BstXI 248 CCAAGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
301 CCAATTGGCTGG
EcoRII 97 CCAGG ^CC[AT]GG
195 CCAGG
EcoT14I 248 CCAAGG C^C[AT][AT]GG
347 CCTTGG
HaeIII 43 GGCC GG^CC
345 GGCC
HapII 51 CCGG C^CGG
84 CCGG
HpaII 51 CCGG C^CGG
84 CCGG
MspI 51 CCGG C^CGG
84 CCGG
MvaI 97 CCAGG CC^[AT]GG
195 CCAGG
NciI 83 CCCGG CC^[GC]GG
84 CCGGG
StyI 248 CCAAGG C^C[AT][AT]GG
347 CCTTGG
Tsp509I 294 AATT ^AATT
303 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AciI BbvI BsaJI BssKI Cac8I
Cfr13I Fnu4HI ItaI MnlI MwoI
NlaIII NlaIV Sau96I ScrFI TseI