Restriction map for
pF1KB8162
##################################
# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwNI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BalI BamHI BanI BbeI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BseAI BsiEI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FbaI FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NarI NheI
NotI NruI PacI PaeR7I PinAI
PleI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI XbaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 65 enzymes) #
###################################
AcsI 1008 GAATTC [GA]^AATT[CT]
AflIII 240 ACACGT A^C[GA][CT]GT
Alw44I 118 GTGCAC G^TGCAC
AlwI 805 GATCC GGATC(4/5)
ApaLI 118 GTGCAC G^TGCAC
ApoI 1008 GAATTC [GA]^AATT[CT]
Asp700I 6 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 738 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 29 CCCGGG C^[CT]CG[GA]G
BbrPI 241 CACGTG CAC^GTG
BbsI 127 GAAGAC GAAGAC(2/6)
BbvCI 142 GCTGAGG CCTCAGC(-5/-2)
BcgI 45 GCAGGAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 127 GAAGAC GAAGAC(2/6)
BsaAI 241 CACGTG [CT]AC^GT[GA]
BsaI 470 GAGACC GGTCTC(1/5)
BsaWI 306 TCCGGT [AT]^CCGG[AT]
BseRI 295 GAGGAG GAGGAG(10/8)
BsiWI 535 CGTACG C^GTACG
BsmAI 470 GAGAC GTCTC(1/5)
BsmI 419 GAATGC GAATGC(1/-1)
BsoBI 29 CCCGGG C^[CT]CG[GA]G
BspMI 161 ACCTGC ACCTGC(4/8)
BsrFI 443 GCCGGC [GA]^CCGG[CT]
BstAPI 361 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 590 TTCGAA TT^CGAA
BstNI 460 CCTGG CC^[AT]GG
CfoI 839 GCGC GCG^C
Cfr10I 443 GCCGGC [GA]^CCGG[CT]
DraII 386 GGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 605 CCATGG C^C[GA][CT]GG
EarI 61 CTCTTC CTCTTC(1/4)
EcoO109I 386 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoRI 1008 GAATTC G^AATTC
EcoRII 460 CCTGG ^CC[AT]GG
EcoT14I 605 CCATGG C^C[AT][AT]GG
FauI 357 GCGGG CCCGC(4/6)
HaeII 838 GGCGCT [GA]GCGC^[CT]
HgaI 918 GCGTC GACGC(5/10)
HhaI 839 GCGC GCG^C
HinP1I 839 GCGC G^CGC
Ksp632I 61 CTCTTC CTCTTC(1/4)
MseI 101 TTAA T^TAA
MvaI 460 CCTGG CC^[AT]GG
NaeI 443 GCCGGC GCC^GGC
NcoI 605 CCATGG C^CATGG
NdeI 641 CATATG CA^TATG
NgoMIV 443 GCCGGC G^CCGGC
NspI 423 GCATGT [GA]CATG^[CT]
NspV 590 TTCGAA TT^CGAA
PflFI 738 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 829 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 241 CACGTG CAC^GTG
PmlI 241 CACGTG CAC^GTG
SfuI 590 TTCGAA TT^CGAA
SmaI 29 CCCGGG CCC^GGG
SmlI 755 CTCAAG C^T[CT][GA]AG
StyI 605 CCATGG C^C[AT][AT]GG
Tru9I 101 TTAA T^TAA
Tth111I 738 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 829 CCAAACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 233 CCAAGTTACACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 29 CCCGGG C^CCGGG
XmaI 29 CCCGGG C^CCGGG
XmnI 6 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 118 GTGCAC G[AT]GC[AT]^C
647 GAGCAC
BanII 909 GAGCCC G[GA]GC[CT]^C
1058 GAGCCC
BsgI 163 CTGCAC GTGCAG(16/14)
427 GTGCAG
BsiHKAI 118 GTGCAC G[AT]GC[AT]^C
647 GAGCAC
BsmFI 902 GGGAC GGGAC(10/14)
744 GTCCC
BsrBI 678 CCGCTC CCGCTC(-3/-3)
355 GAGCGG
BsrI 509 ACTGG ACTGG(1/-1)
526 CCAGT
BstF5I 147 GGATG GGATG(2/0)
366 GGATG
DpnII 532 GATC ^GATC
805 GATC
EcoT22I 421 ATGCAT ATGCA^T
638 ATGCAT
FokI 147 GGATG GGATG(9/13)
366 GGATG
HindIII 175 AAGCTT A^AGCTT
224 AAGCTT
HinfI 51 GATTC G^A[ACGT]TC
619 GAATC
HphI 3 GGTGA GGTGA(8/7)
186 GGTGA
MaeII 197 ACGT A^CGT
242 ACGT
MboI 532 GATC ^GATC
805 GATC
MboII 127 GAAGA GAAGA(8/7)
62 TCTTC
MslI 192 CATCAACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
768 CACAACTGTG
NdeII 532 GATC ^GATC
805 GATC
NsiI 421 ATGCAT ATGCA^T
638 ATGCAT
PstI 872 CTGCAG CTGCA^G
926 CTGCAG
PvuII 140 CAGCTG CAG^CTG
854 CAGCTG
Sau3AI 532 GATC ^GATC
805 GATC
TfiI 51 GATTC G^A[AT]TC
619 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BcnI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI Cac8I Cfr13I DdeI
Fnu4HI HaeIII HapII HpaII ItaI
MaeIII MnlI MspA1I MspI MwoI
NciI NlaIII NlaIV RsaI Sau96I
ScrFI SfaNI SfcI TaqI TseI
Tsp45I Tsp509I TspRI TthHB8I