Restriction map for
pF1KB8159
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BfaI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaBI BsaI BseRI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII ClaI
CpoI DraI DraII DraIII DrdI
EarI Eco47III Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MluNI MscI MseI MslI
MspA1I MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 201 CGCG CG^CG
AccIII 263 TCCGGA T^CCGGA
AhdI 267 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 267 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 354 GGTCC G^G[AT]CC
BclI 218 TGATCA T^GATCA
BsaAI 19 TACGTG [CT]AC^GT[GA]
BsaWI 263 TCCGGA [AT]^CCGG[AT]
BseAI 263 TCCGGA T^CCGGA
BsgI 249 CTGCAC GTGCAG(16/14)
BsiEI 104 CGGCCG CG[GA][CT]^CG
BsiYI 101 CCACGGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 101 CCACGGCCGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 407 GAGAC GTCTC(1/5)
BspEI 263 TCCGGA T^CCGGA
BsrFI 4 GCCGGT [GA]^CCGG[CT]
BstF5I 261 CATCC GGATG(2/0)
BstNI 51 CCAGG CC^[AT]GG
BstUI 201 CGCG CG^CG
CfoI 200 GCGC GCG^C
Cfr10I 4 GCCGGT [GA]^CCGG[CT]
Cfr13I 354 GGTCC G^G[ACGT]CC
DdeI 213 CTCAG C^T[ACGT]AG
DpnII 219 GATC ^GATC
EagI 104 CGGCCG C^GGCCG
Eam1105I 267 GACAAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 104 CGGCCG C^GGCCG
Eco52I 104 CGGCCG C^GGCCG
EcoRII 51 CCAGG ^CC[AT]GG
EcoT14I 357 CCATGG C^C[AT][AT]GG
FbaI 218 TGATCA T^GATCA
FokI 261 CATCC GGATG(9/13)
HhaI 200 GCGC GCG^C
HinP1I 200 GCGC G^CGC
HphI 244 GGTGA GGTGA(8/7)
MaeIII 245 GTGAC ^GT[ACGT]AC
MboI 219 GATC ^GATC
MboII 204 GAAGA GAAGA(8/7)
MroI 263 TCCGGA T^CCGGA
MvaI 51 CCAGG CC^[AT]GG
MvnI 201 CGCG CG^CG
NcoI 357 CCATGG C^CATGG
NdeII 219 GATC ^GATC
NlaIII 358 CATG CATG^
Sau3AI 219 GATC ^GATC
Sau96I 354 GGTCC G^G[ACGT]CC
SmlI 402 CTTGAG C^T[CT][GA]AG
StyI 357 CCATGG C^C[AT][AT]GG
TaqI 239 TCGA T^CGA
Tsp45I 245 GTGAC ^GT[GC]AC
TspRI 215 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 239 TCGA T^CGA
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# TWO CUT ENZYMES ( 13 enzymes) #
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AciI 59 CCGC CCGC(-3/-1)
365 GCGG
AcyI 79 GACGCC G[GA]^CG[CT]C
112 GGCGTC
AluI 16 AGCT AG^CT
315 AGCT
BcnI 107 CCGGG CC^[GC]GG
165 CCGGG
BsaHI 79 GACGCC G[GA]^CG[CT]C
112 GGCGTC
DsaI 101 CCACGG C^C[GA][CT]GG
357 CCATGG
EaeI 2 TGGCCG [CT]^GGCC[GA]
104 CGGCCG
HgaI 79 GACGC GACGC(5/10)
113 GCGTC
Hin1I 79 GACGCC G[GA]^CG[CT]C
112 GGCGTC
MnlI 55 GAGG CCTC(7/6)
362 GAGG
MwoI 38 GCGATGGCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
97 GCAGCCACGGC
NciI 107 CCGGG CC^[GC]GG
165 CCGGG
NlaIV 285 GGAGCC GG[ACGT]^[ACGT]CC
353 GGGTCC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
#########################################
BbvI BsaJI BssKI Fnu4HI HaeIII
HapII HinfI HpaII ItaI MaeII
MspI PleI ScrFI TseI