Restriction map for
pF1KB8155
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# NO CUT ENZYMES ( 102 enzymes) #
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Acc65I AccI AclI AcsI AflII
AflIII AgeI AocI Aor51HI ApoI
AscI AseI AsnI Asp718I AvrII
BamHI BbrPI BcgI BclI BfrI
BlnI BsaBI BsaI BsiWI BsmBI
BsmI Bsp1407I BspDI BspLU11I BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SspBI
SspI StyI SwaI Tru9I Tsp509I
XbaI XhoI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AatII 1369 GACGTC GACGT^C
AfaI 1104 GTAC GT^AC
AhdI 766 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1297 GTGCAC G^TGCAC
ApaI 225 GGGCCC GGGCC^C
ApaLI 1297 GTGCAC G^TGCAC
Asp700I 987 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 766 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 175 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 818 TGGCCA TGG^CCA
BanI 64 GGCGCC G^G[CT][GA]CC
BbeI 64 GGCGCC GGCGC^C
BbsI 1312 GTCTTC GAAGAC(2/6)
BbvCI 55 CCTCAGC CCTCAGC(-5/-2)
BciVI 27 GGATAC GTATCC(6/5)
BfaI 937 CTAG C^TAG
BglI 374 GCCGGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1361 AGATCT A^GATCT
BpmI 1327 CTCCAG CTGGAG(16/14)
BpuAI 1312 GTCTTC GAAGAC(2/6)
BsaAI 1192 TACGTG [CT]AC^GT[GA]
BseRI 94 GAGGAG GAGGAG(10/8)
BspHI 1262 TCATGA T^CATGA
DraII 224 AGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 127 GACCCTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1136 CGGCCG C^GGCCG
Eam1105I 766 GACCCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 196 GAAGAG CTCTTC(1/4)
EclXI 1136 CGGCCG C^GGCCG
Eco52I 1136 CGGCCG C^GGCCG
EcoNI 903 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 224 AGGGCCC [GA]G^G[ACGT]CC[CT]
KasI 64 GGCGCC G^GCGCC
Ksp632I 196 GAAGAG CTCTTC(1/4)
MaeI 937 CTAG C^TAG
MluNI 818 TGGCCA TGG^CCA
MscI 818 TGGCCA TGG^CCA
MslI 1257 CATGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 431 GCCGGC GCC^GGC
NarI 64 GGCGCC GG^CGCC
NgoMIV 431 GCCGGC G^CCGGC
NspI 1256 GCATGC [GA]CATG^[CT]
PflFI 175 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 681 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 526 GAGTC GAGTC(4/5)
RcaI 1262 TCATGA T^CATGA
RsaI 1104 GTAC GT^AC
SacI 1290 GAGCTC GAGCT^C
SbfI 1029 CCTGCAGG CCTGCA^GG
SfoI 64 GGCGCC GGC^GCC
SmaI 115 CCCGGG CCC^GGG
SmlI 665 CTCAAG C^T[CT][GA]AG
SphI 1256 GCATGC GCATG^C
Sse8387I 1029 CCTGCAGG CCTGCA^GG
TfiI 507 GATTC G^A[AT]TC
Tth111I 175 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 681 CCAGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 115 CCCGGG C^CCGGG
XmaI 115 CCCGGG C^CCGGG
XmnI 987 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
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AcyI 64 GGCGCC G[GA]^CG[CT]C
1369 GACGTC
AlwNI 140 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
578 CAGCCTCTG
AvaI 115 CCCGGG C^[CT]CG[GA]G
776 CCCGAG
AviII 437 TGCGCA TGC^GCA
1280 TGCGCA
BsaHI 64 GGCGCC G[GA]^CG[CT]C
1369 GACGTC
BsgI 1022 GTGCAG GTGCAG(16/14)
1153 GTGCAG
BsiEI 1136 CGGCCG CG[GA][CT]^CG
1140 CGACCG
BsmAI 940 GAGAC GTCTC(1/5)
964 GAGAC
BsoBI 115 CCCGGG C^[CT]CG[GA]G
776 CCCGAG
BsrFI 431 GCCGGC [GA]^CCGG[CT]
1142 ACCGGC
BsrI 176 ACTGG ACTGG(1/-1)
771 CCAGT
Cfr10I 431 GCCGGC [GA]^CCGG[CT]
1142 ACCGGC
DsaI 109 CCGTGG C^C[GA][CT]GG
348 CCGTGG
EaeI 818 TGGCCA [CT]^GGCC[GA]
1136 CGGCCG
FspI 437 TGCGCA TGC^GCA
1280 TGCGCA
Hin1I 64 GGCGCC G[GA]^CG[CT]C
1369 GACGTC
HinfI 507 GATTC G^A[ACGT]TC
526 GAGTC
PvuII 306 CAGCTG CAG^CTG
1240 CAGCTG
StuI 278 AGGCCT AGG^CCT
1026 AGGCCT
TaqI 923 TCGA T^CGA
1058 TCGA
TthHB8I 923 TCGA T^CGA
1058 TCGA
XcmI 14 CCACGAGTTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1073 CCATGCGGCGGCTGG
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# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AccIII AciI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BlpI BmyI Bpu1102I BsaJI BsaWI
BseAI BsiHKAI BsiYI BslI BsmFI
Bsp1286I BspEI BspMI BssKI BssSI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI MaeII MaeIII
MboI MboII MflI MnlI MroI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI Tsp45I TspRI XhoII