Restriction map for
pF1KB8154
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AvaII AviII AvrII BalI BamHI
BanI BbeI BbrPI BbvCI BcgI
BcnI BfaI BfrI BglI BglII
BlnI BsaAI BsaHI BsaWI BseAI
BseRI BsgI BsiEI BsiWI BsmBI
BsmFI BsoBI BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
FauI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HindIII
HpaI HpaII KasI KpnI KspI
MaeI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NgoMIV NheI
NlaIV NotI NruI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PstI PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AclI 741 AACGTT AA^CGTT
Alw44I 1595 GTGCAC G^TGCAC
AlwI 1513 GGATC GGATC(4/5)
ApaLI 1595 GTGCAC G^TGCAC
AseI 423 ATTAAT AT^TAAT
AsnI 423 ATTAAT AT^TAAT
BbvI 1590 GCAGC GCAGC(8/12)
BclI 1269 TGATCA T^GATCA
BlpI 1018 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1018 GCTCAGC GC^T[ACGT]AGC
BsaBI 1270 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI 228 CCCTGATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 228 CCCTGATTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BspHI 1611 TCATGA T^CATGA
BstBI 970 TTCGAA TT^CGAA
BstXI 5 CCAAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1513 GGATCT [GA]^GATC[CT]
CelII 1018 GCTCAGC GC^T[ACGT]AGC
CfoI 766 GCGC GCG^C
DraI 247 TTTAAA TTT^AAA
EarI 683 CTCTTC CTCTTC(1/4)
EcoT14I 5 CCAAGG C^C[AT][AT]GG
EcoT22I 719 ATGCAT ATGCA^T
FbaI 1269 TGATCA T^GATCA
Fnu4HI 1590 GCAGC GC^[ACGT]GC
HhaI 766 GCGC GCG^C
HinP1I 766 GCGC G^CGC
ItaI 1590 GCAGC GC^[ACGT]GC
Ksp632I 683 CTCTTC CTCTTC(1/4)
MaeII 742 ACGT A^CGT
MamI 1270 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1513 GGATCT [GA]^GATC[CT]
MslI 550 CATAAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 391 CAGCTG C[AC]G^C[GT]G
NdeI 281 CATATG CA^TATG
NsiI 719 ATGCAT ATGCA^T
NspI 721 GCATGT [GA]CATG^[CT]
NspV 970 TTCGAA TT^CGAA
PshBI 423 ATTAAT AT^TAAT
Psp1406I 741 AACGTT AA^CGTT
PvuII 391 CAGCTG CAG^CTG
RcaI 1611 TCATGA T^CATGA
SacI 669 GAGCTC GAGCT^C
SfuI 970 TTCGAA TT^CGAA
SmlI 47 CTCAAG C^T[CT][GA]AG
StyI 5 CCAAGG C^C[AT][AT]GG
TaqI 971 TCGA T^CGA
TfiI 289 GAATC G^A[AT]TC
TseI 1590 GCAGC G^C[AT]GC
TthHB8I 971 TCGA T^CGA
XcmI 223 CCATGCCCTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1513 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AspHI 669 GAGCTC G[AT]GC[AT]^C
1595 GTGCAC
BanII 187 GAGCCC G[GA]GC[CT]^C
669 GAGCTC
BbsI 358 GAAGAC GAAGAC(2/6)
658 GTCTTC
BciVI 501 GGATAC GTATCC(6/5)
1093 GGATAC
BpuAI 358 GAAGAC GAAGAC(2/6)
658 GTCTTC
BsaI 963 GAGACC GGTCTC(1/5)
1131 GAGACC
BsaJI 5 CCAAGG C^C[ACGT][ACGT]GG
190 CCCTGG
BsiHKAI 669 GAGCTC G[AT]GC[AT]^C
1595 GTGCAC
BsmI 837 GAATGC GAATGC(1/-1)
1425 GAATGC
Bsp1407I 89 TGTACA T^GTACA
1104 TGTACA
BsrGI 89 TGTACA T^GTACA
1104 TGTACA
Cac8I 1429 GCAGGC GC[ACGT]^[ACGT]GC
1593 GCGTGC
Cfr13I 220 GGCCC G^G[ACGT]CC
849 GGCCC
MseI 248 TTAA T^TAA
424 TTAA
Sau96I 220 GGCCC G^G[ACGT]CC
849 GGCCC
SfaNI 939 GCATC GCATC(5/9)
1023 GCATC
SspBI 89 TGTACA T^GTACA
1104 TGTACA
Tru9I 248 TTAA T^TAA
424 TTAA
Tsp45I 16 GTGAC ^GT[GC]AC
304 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BmyI BpmI BsmAI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI DdeI
DpnII EcoRI EcoRII FokI HaeIII
HinfI HphI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
PleI RsaI Sau3AI ScrFI SfcI
StuI Tsp509I TspRI XmnI