Restriction map for
pF1KB8153
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AclI AfaI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspHI AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BsaAI
BsaBI BsaI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoRI EcoRV EcoT14I EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MamI MfeI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsaI RsrII SacI SalI
SapI ScaI SfiI SmlI SnaBI
SpeI SphI SspBI SspI StuI
StyI SwaI Tsp45I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 643 GAATTT [GA]^AATT[CT]
AhdI 97 GACGACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 643 GAATTT [GA]^AATT[CT]
AseI 1075 ATTAAT AT^TAAT
AsnI 1075 ATTAAT AT^TAAT
AspEI 97 GACGACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 71 TGGCCA TGG^CCA
BanII 219 GAGCCC G[GA]GC[CT]^C
BbsI 688 GAAGAC GAAGAC(2/6)
BfaI 365 CTAG C^TAG
BglI 323 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 688 GAAGAC GAAGAC(2/6)
BsgI 758 CTGCAC GTGCAG(16/14)
BsrBI 430 GAGCGG CCGCTC(-3/-3)
BsrI 740 CCAGT ACTGG(1/-1)
BstAPI 150 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 713 TTCGAA TT^CGAA
BstEII 1084 GGTAACC G^GT[ACGT]ACC
BstPI 1084 GGTAACC G^GT[ACGT]ACC
BstYI 436 AGATCC [GA]^GATC[CT]
DraII 229 GGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 97 GACGACGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 229 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1084 GGTAACC G^GT[ACGT]ACC
HgaI 616 GACGC GACGC(5/10)
KspI 207 CCGCGG CCGC^GG
MaeI 365 CTAG C^TAG
MaeII 552 ACGT A^CGT
MaeIII 1085 GTAAC ^GT[ACGT]AC
MflI 436 AGATCC [GA]^GATC[CT]
MluNI 71 TGGCCA TGG^CCA
MscI 71 TGGCCA TGG^CCA
MslI 777 CACAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspV 713 TTCGAA TT^CGAA
PshBI 1075 ATTAAT AT^TAAT
SacII 207 CCGCGG CCGC^GG
SbfI 336 CCTGCAGG CCTGCA^GG
SexAI 513 ACCAGGT A^CC[AT]GGT
SfuI 713 TTCGAA TT^CGAA
SgrAI 139 CGCCGGCG C[GA]^CCGG[CT]G
Sse8387I 336 CCTGCAGG CCTGCA^GG
XcmI 1169 CCAGAGCAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 436 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccIII 932 TCCGGA T^CCGGA
1037 TCCGGA
AlwNI 88 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
151 CAGCAGCTG
BlpI 307 GCTCAGC GC^T[ACGT]AGC
427 GCTGAGC
BmyI 219 GAGCCC G[AGT]GC[ACT]^C
798 GGGCAC
BpmI 818 CTCCAG CTGGAG(16/14)
1069 CTGGAG
Bpu1102I 307 GCTCAGC GC^T[ACGT]AGC
427 GCTGAGC
BsaWI 932 TCCGGA [AT]^CCGG[AT]
1037 TCCGGA
BseAI 932 TCCGGA T^CCGGA
1037 TCCGGA
BsiEI 191 CGGCCG CG[GA][CT]^CG
258 CGGCCG
Bsp1286I 219 GAGCCC G[AGT]GC[ACT]^C
798 GGGCAC
BspEI 932 TCCGGA T^CCGGA
1037 TCCGGA
CelII 307 GCTCAGC GC^T[ACGT]AGC
427 GCTGAGC
EagI 191 CGGCCG C^GGCCG
258 CGGCCG
EarI 368 GAAGAG CTCTTC(1/4)
808 GAAGAG
EclXI 191 CGGCCG C^GGCCG
258 CGGCCG
Eco52I 191 CGGCCG C^GGCCG
258 CGGCCG
EcoNI 123 CCTGCGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
364 CCTAGAAGAGG
Ksp632I 368 GAAGAG CTCTTC(1/4)
808 GAAGAG
MroI 932 TCCGGA T^CCGGA
1037 TCCGGA
MseI 926 TTAA T^TAA
1076 TTAA
NaeI 140 GCCGGC GCC^GGC
247 GCCGGC
NgoMIV 140 GCCGGC G^CCGGC
247 GCCGGC
NlaIII 696 CATG CATG^
1030 CATG
PstI 157 CTGCAG CTGCA^G
337 CTGCAG
SmaI 325 CCCGGG CCC^GGG
1124 CCCGGG
Tru9I 926 TTAA T^TAA
1076 TTAA
Tsp509I 644 AATT ^AATT
1078 AATT
XmaCI 325 CCCGGG C^CCGGG
1124 CCCGGG
XmaI 325 CCCGGG C^CCGGG
1124 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AvaI AvaII BanI BbeI BbvI
BcnI BsaHI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI BsrFI
BssKI BstF5I BstNI BstUI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NarI NciI NdeII NlaIV
PleI PvuII Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI TaqI TfiI
TseI TspRI TthHB8I