Restriction map for
pF1KB8150
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaII AvrII BalI
BanI BbeI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
HaeII HapII HgaI Hin1I HincII
HindII HindIII HpaI HpaII KasI
KpnI KspI MamI MluI MluNI
MroI MscI MslI MspI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NotI NruI NsiI PacI
PaeR7I PflFI PflMI PinAI PleI
PmeI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 1103 GTAGAC GT^[AC][GT]AC
AciI 913 GCGG CCGC(-3/-1)
AfaI 871 GTAC GT^AC
AflIII 570 ACGTGT A^C[GA][CT]GT
AocI 670 CCTGAGG CC^T[ACGT]AGG
ApaI 639 GGGCCC GGGCC^C
AseI 1201 ATTAAT AT^TAAT
AsnI 1201 ATTAAT AT^TAAT
AvaI 194 CCCGAG C^[CT]CG[GA]G
AviII 1016 TGCGCA TGC^GCA
BamHI 190 GGATCC G^GATCC
BbrPI 455 CACGTG CAC^GTG
BsaAI 455 CACGTG [CT]AC^GT[GA]
BsaI 900 GGTCTC GGTCTC(1/5)
BsmAI 901 GTCTC GTCTC(1/5)
BsmI 992 GAATGC GAATGC(1/-1)
BsoBI 194 CCCGAG C^[CT]CG[GA]G
BssSI 596 CTCGTG CACGAG(-5/-1)
BstBI 1314 TTCGAA TT^CGAA
Bsu36I 670 CCTGAGG CC^T[ACGT]AGG
CfoI 1017 GCGC GCG^C
Eco81I 670 CCTGAGG CC^T[ACGT]AGG
EcoNI 1054 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 1016 TGCGCA TGC^GCA
HhaI 1017 GCGC GCG^C
HinP1I 1017 GCGC G^CGC
MfeI 816 CAATTG C^AATTG
MunI 816 CAATTG C^AATTG
NheI 1257 GCTAGC G^CTAGC
NspV 1314 TTCGAA TT^CGAA
PmaCI 455 CACGTG CAC^GTG
PmlI 455 CACGTG CAC^GTG
PshBI 1201 ATTAAT AT^TAAT
RsaI 871 GTAC GT^AC
SacI 757 GAGCTC GAGCT^C
SfuI 1314 TTCGAA TT^CGAA
SphI 83 GCATGC GCATG^C
Tsp45I 1339 GTGAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AlwNI 272 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
665 CAGAGCCTG
AspHI 599 GTGCTC G[AT]GC[AT]^C
757 GAGCTC
BanII 639 GGGCCC G[GA]GC[CT]^C
757 GAGCTC
BbsI 151 GAAGAC GAAGAC(2/6)
247 GAAGAC
BpmI 662 CTCCAG CTGGAG(16/14)
1231 CTGGAG
BpuAI 151 GAAGAC GAAGAC(2/6)
247 GAAGAC
BseRI 754 GAGGAG GAGGAG(10/8)
1234 GAGGAG
BsiHKAI 599 GTGCTC G[AT]GC[AT]^C
757 GAGCTC
BsiYI 1051 CCTCCTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1054 CCTGGGGGAGG
BslI 1051 CCTCCTGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1054 CCTGGGGGAGG
BsmFI 608 GGGAC GGGAC(10/14)
1411 GGGAC
DraII 638 AGGGCCC [GA]G^G[ACGT]CC[CT]
1132 GGGGCCT
EarI 697 GAAGAG CTCTTC(1/4)
961 GAAGAG
EcoO109I 638 AGGGCCC [GA]G^G[ACGT]CC[CT]
1132 GGGGCCT
EcoT14I 520 CCAAGG C^C[AT][AT]GG
896 CCAAGG
Ksp632I 697 GAAGAG CTCTTC(1/4)
961 GAAGAG
NspI 83 GCATGC [GA]CATG^[CT]
347 ACATGC
PstI 398 CTGCAG CTGCA^G
1028 CTGCAG
SfcI 398 CTGCAG C^T[GA][CT]AG
1028 CTGCAG
SmlI 283 CTTGAG C^T[CT][GA]AG
784 CTTGAG
SpeI 873 ACTAGT A^CTAGT
1246 ACTAGT
StyI 520 CCAAGG C^C[AT][AT]GG
896 CCAAGG
TaqI 852 TCGA T^CGA
1315 TCGA
TthHB8I 852 TCGA T^CGA
1315 TCGA
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# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
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AcsI AluI AlwI ApoI BbvI
BfaI BmyI BsaJI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PvuII
Sau3AI Sau96I ScrFI SfaNI TfiI
Tru9I TseI Tsp509I TspRI XhoII