Restriction map for
pF1KB8148
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AocI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BalI
BamHI BbrPI BcgI BciVI BclI
BfaI BfrI BlnI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MfeI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI ScaI SexAI
SfiI SfuI SnaBI SpeI SspBI
SspI StuI SwaI Tru9I Tth111I
Van91I XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 1000 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BbsI 141 GTCTTC GAAGAC(2/6)
BglI 595 GCCGACGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 725 AGATCT A^GATCT
BpuAI 141 GTCTTC GAAGAC(2/6)
BsaAI 52 TACGTG [CT]AC^GT[GA]
BsmBI 58 GAGACG CGTCTC(1/5)
BsrBI 1308 CCGCTC CCGCTC(-3/-3)
BssSI 325 CACGAG CACGAG(-5/-1)
DsaI 731 CCGTGG C^C[GA][CT]GG
EcoNI 216 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MaeIII 745 GTGAC ^GT[ACGT]AC
MslI 1206 CACCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 569 GCCGGC GCC^GGC
NgoMIV 569 GCCGGC G^CCGGC
PaeR7I 625 CTCGAG C^TCGAG
SbfI 519 CCTGCAGG CCTGCA^GG
SgrAI 134 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 65 CCCGGG CCC^GGG
SphI 961 GCATGC GCATG^C
Sse8387I 519 CCTGCAGG CCTGCA^GG
TfiI 207 GAATC G^A[AT]TC
Tsp45I 745 GTGAC ^GT[GC]AC
TspRI 1227 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoI 625 CTCGAG C^TCGAG
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AhdI 244 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
907 GACGAGGTGTC
Aor51HI 981 AGCGCT AGC^GCT
1033 AGCGCT
AspEI 244 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
907 GACGAGGTGTC
AvaI 65 CCCGGG C^[CT]CG[GA]G
625 CTCGAG
AviII 86 TGCGCA TGC^GCA
512 TGCGCA
BanI 132 GGCGCC G^G[CT][GA]CC
592 GGCGCC
BbeI 132 GGCGCC GGCGC^C
592 GGCGCC
BbvCI 826 GCTGAGG CCTCAGC(-5/-2)
1387 GCTGAGG
BlpI 110 GCTCAGC GC^T[ACGT]AGC
546 GCTGAGC
Bpu1102I 110 GCTCAGC GC^T[ACGT]AGC
546 GCTGAGC
BsaI 505 GAGACC GGTCTC(1/5)
1189 GAGACC
BsoBI 65 CCCGGG C^[CT]CG[GA]G
625 CTCGAG
BspMI 434 ACCTGC ACCTGC(4/8)
518 ACCTGC
BsrFI 135 GCCGGT [GA]^CCGG[CT]
569 GCCGGC
BstXI 941 CCAAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1248 CCAGGTCTTTGG
CelII 110 GCTCAGC GC^T[ACGT]AGC
546 GCTGAGC
Cfr10I 135 GCCGGT [GA]^CCGG[CT]
569 GCCGGC
DraII 284 AGGACCT [GA]G^G[ACGT]CC[CT]
347 AGGTCCT
EaeI 803 TGGCCG [CT]^GGCC[GA]
1257 TGGCCG
Eam1105I 244 GACCTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
907 GACGAGGTGTC
Eco47III 981 AGCGCT AGC^GCT
1033 AGCGCT
EcoO109I 284 AGGACCT [GA]G^G[ACGT]CC[CT]
347 AGGTCCT
FauI 398 CCCGC CCCGC(4/6)
1307 CCCGC
FspI 86 TGCGCA TGC^GCA
512 TGCGCA
KasI 132 GGCGCC G^GCGCC
592 GGCGCC
NarI 132 GGCGCC GG^CGCC
592 GGCGCC
NlaIV 132 GGCGCC GG[ACGT]^[ACGT]CC
592 GGCGCC
NspI 815 ACATGC [GA]CATG^[CT]
961 GCATGC
PleI 1196 GACTC GAGTC(4/5)
1501 GAGTC
PpuMI 284 AGGACCT [GA]G^G[AT]CC[CT]
347 AGGTCCT
PvuII 994 CAGCTG CAG^CTG
1472 CAGCTG
SacI 622 GAGCTC GAGCT^C
1242 GAGCTC
SfoI 132 GGCGCC GGC^GCC
592 GGCGCC
Tsp509I 1058 AATT ^AATT
1070 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI ApaLI AspHI AvaII
BanII BbvI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI EcoRII EcoT14I Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MboI MboII
MflI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI TthHB8I XhoII