Restriction map for
pF1KB8132
##################################
# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BalI BamHI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsgI BsiWI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI HphI
KpnI MaeII MaeIII MamI MfeI
MluI MluNI MscI MseI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NsiI NspI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SalI SbfI
ScaI SexAI SfcI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tru9I
Tsp45I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 166 GTCTAC GT^[AC][GT]AC
AccIII 564 TCCGGA T^CCGGA
AcsI 160 GAATTT [GA]^AATT[CT]
AhdI 1018 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 238 CCTGAGG CC^T[ACGT]AGG
ApoI 160 GAATTT [GA]^AATT[CT]
Asp700I 1341 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1018 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbvCI 667 CCTCAGC CCTCAGC(-5/-2)
BsaI 103 GAGACC GGTCTC(1/5)
BseAI 564 TCCGGA T^CCGGA
BsiEI 1308 CGGCCG CG[GA][CT]^CG
BsmBI 1152 CGTCTC CGTCTC(1/5)
BspEI 564 TCCGGA T^CCGGA
BssHII 244 GCGCGC G^CGCGC
BssSI 1387 CACGAG CACGAG(-5/-1)
BstXI 510 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 238 CCTGAGG CC^T[ACGT]AGG
EaeI 1308 CGGCCG [CT]^GGCC[GA]
EagI 1308 CGGCCG C^GGCCG
Eam1105I 1018 GACGCGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1308 CGGCCG C^GGCCG
Eco52I 1308 CGGCCG C^GGCCG
Eco81I 238 CCTGAGG CC^T[ACGT]AGG
MroI 564 TCCGGA T^CCGGA
NcoI 1302 CCATGG C^CATGG
NruI 497 TCGCGA TCG^CGA
PaeR7I 1144 CTCGAG C^TCGAG
PflMI 192 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmaI 1421 CCCGGG CCC^GGG
SmlI 1144 CTCGAG C^T[CT][GA]AG
StuI 1259 AGGCCT AGG^CCT
TaqI 1145 TCGA T^CGA
TthHB8I 1145 TCGA T^CGA
Van91I 192 CCAGACCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1293 CCACAGCAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1144 CTCGAG C^TCGAG
XmaCI 1421 CCCGGG C^CCGGG
XmaI 1421 CCCGGG C^CCGGG
XmnI 1341 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AfaI 336 GTAC GT^AC
456 GTAC
Aor51HI 859 AGCGCT AGC^GCT
1316 AGCGCT
BbeI 921 GGCGCC GGCGC^C
1417 GGCGCC
BbsI 1341 GAAGAC GAAGAC(2/6)
1380 GAAGAC
BglI 1142 GCCTCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1301 GCCATGGCGGC
BpuAI 1341 GAAGAC GAAGAC(2/6)
1380 GAAGAC
BsrI 1098 ACTGG ACTGG(1/-1)
225 CCAGT
Eco47III 859 AGCGCT AGC^GCT
1316 AGCGCT
EcoNI 26 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
366 CCTGGTGGAGG
KasI 921 GGCGCC G^GCGCC
1417 GGCGCC
KspI 890 CCGCGG CCGC^GG
1224 CCGCGG
MspA1I 890 CCGCGG C[AC]G^C[GT]G
1224 CCGCGG
NarI 921 GGCGCC GG^CGCC
1417 GGCGCC
RsaI 336 GTAC GT^AC
456 GTAC
SacI 36 GAGCTC GAGCT^C
493 GAGCTC
SacII 890 CCGCGG CCGC^GG
1224 CCGCGG
SfoI 921 GGCGCC GGC^GCC
1417 GGCGCC
Tsp509I 161 AATT ^AATT
438 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AcyI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaHI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII ItaI Ksp632I MaeI MboI
MboII MflI MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI SapI Sau3AI
Sau96I ScrFI SfaNI StyI TseI
TspRI XhoII