Restriction map for
pF1KB8131
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmI
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII Hin1I
HindIII HpaI HphI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MspA1I MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
StyI SwaI Tru9I TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 241 GTCTAC GT^[AC][GT]AC
AluI 265 AGCT AG^CT
AlwI 162 GATCC GGATC(4/5)
AvaI 30 CTCGGG C^[CT]CG[GA]G
BlpI 502 GCTCAGC GC^T[ACGT]AGC
BpmI 157 CTGGAG CTGGAG(16/14)
Bpu1102I 502 GCTCAGC GC^T[ACGT]AGC
BsaJI 225 CCAGGG C^C[ACGT][ACGT]GG
BsgI 61 GTGCAG GTGCAG(16/14)
BsoBI 30 CTCGGG C^[CT]CG[GA]G
Bsp1407I 209 TGTACA T^GTACA
BsrFI 73 ACCGGC [GA]^CCGG[CT]
BsrGI 209 TGTACA T^GTACA
BsrI 328 CCAGT ACTGG(1/-1)
BstYI 161 AGATCC [GA]^GATC[CT]
Cac8I 175 GCGGGC GC[ACGT]^[ACGT]GC
CelII 502 GCTCAGC GC^T[ACGT]AGC
CfoI 3 GCGC GCG^C
Cfr10I 73 ACCGGC [GA]^CCGG[CT]
DraII 203 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 222 CGGCCA [CT]^GGCC[GA]
EarI 406 GAAGAG CTCTTC(1/4)
EcoO109I 203 GGGACCT [GA]G^G[ACGT]CC[CT]
HaeIII 223 GGCC GG^CC
HapII 74 CCGG C^CGG
HgaI 193 GCGTC GACGC(5/10)
HhaI 3 GCGC GCG^C
HinP1I 3 GCGC G^CGC
HincII 253 GTCAAC GT[CT]^[GA]AC
HindII 253 GTCAAC GT[CT]^[GA]AC
HinfI 139 GATTC G^A[ACGT]TC
HpaII 74 CCGG C^CGG
Ksp632I 406 GAAGAG CTCTTC(1/4)
MboII 406 GAAGA GAAGA(8/7)
MflI 161 AGATCC [GA]^GATC[CT]
MslI 300 CATCCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 74 CCGG C^CGG
NspI 535 GCATGT [GA]CATG^[CT]
PpuMI 203 GGGACCT [GA]G^G[AT]CC[CT]
SfcI 243 CTACAG C^T[GA][CT]AG
SspBI 209 TGTACA T^GTACA
TfiI 139 GATTC G^A[AT]TC
Tsp45I 70 GTGAC ^GT[GC]AC
Tsp509I 477 AATT ^AATT
XhoII 161 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AfaI 9 GTAC GT^AC
210 GTAC
BanI 76 GGCACC G^G[CT][GA]CC
178 GGCACC
BanII 27 GGGCTC G[GA]GC[CT]^C
395 GAGCCC
BbvI 416 GCTGC GCAGC(8/12)
499 GCTGC
BsiYI 165 CCTGGACACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
420 CCCCTTTATGG
BslI 165 CCTGGACACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
420 CCCCTTTATGG
BsmFI 203 GGGAC GGGAC(10/14)
290 GGGAC
BstF5I 234 CATCC GGATG(2/0)
300 CATCC
DdeI 440 CTAAG C^T[ACGT]AG
503 CTCAG
Fnu4HI 416 GCTGC GC^[ACGT]GC
499 GCTGC
FokI 234 CATCC GGATG(9/13)
300 CATCC
ItaI 416 GCTGC GC^[ACGT]GC
499 GCTGC
MaeIII 70 GTGAC ^GT[ACGT]AC
539 GTAAC
MwoI 35 GCGGGGTAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
147 GCCGTCGGTGC
RsaI 9 GTAC GT^AC
210 GTAC
TseI 416 GCTGC G^C[AT]GC
499 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AccII AciI AvaII BcgI BmyI
Bsp1286I BssKI BstNI BstUI Cfr13I
DpnII EcoRII FauI MboI MnlI
MvaI MvnI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI TaqI TthHB8I