Restriction map for
pF1KB8127
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AccI AccII AccIII AclI AflII
AgeI AhdI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I AspEI AspI AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HincII HindII HindIII
HpaI KasI Ksp632I KspI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tth111I
XbaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AatII 145 GACGTC GACGT^C
Acc65I 479 GGTACC G^GTACC
AcsI 338 AAATTC [GA]^AATT[CT]
AcyI 145 GACGTC G[GA]^CG[CT]C
AflIII 1235 ACGTGT A^C[GA][CT]GT
ApaI 1053 GGGCCC GGGCC^C
ApoI 338 AAATTC [GA]^AATT[CT]
Asp718I 479 GGTACC G^GTACC
BamHI 1016 GGATCC G^GATCC
BbvCI 1103 CCTCAGC CCTCAGC(-5/-2)
BpmI 460 CTGGAG CTGGAG(16/14)
BsaHI 145 GACGTC G[GA]^CG[CT]C
BsaI 926 GAGACC GGTCTC(1/5)
BsaWI 632 ACCGGA [AT]^CCGG[AT]
BspMI 1097 ACCTGC ACCTGC(4/8)
BsrBI 823 CCGCTC CCGCTC(-3/-3)
BsrFI 811 ACCGGC [GA]^CCGG[CT]
BstXI 863 CCACGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1016 GGATCC [GA]^GATC[CT]
Cfr10I 811 ACCGGC [GA]^CCGG[CT]
DdeI 1104 CTCAG C^T[ACGT]AG
HgaI 585 GACGC GACGC(5/10)
Hin1I 145 GACGTC G[GA]^CG[CT]C
HinfI 320 GATTC G^A[ACGT]TC
KpnI 479 GGTACC GGTAC^C
MflI 1016 GGATCC [GA]^GATC[CT]
PaeR7I 1213 CTCGAG C^TCGAG
PflMI 837 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 861 GACCACGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1288 GAGCTC GAGCT^C
TfiI 320 GATTC G^A[AT]TC
Van91I 837 CCAGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1213 CTCGAG C^TCGAG
XhoII 1016 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AspHI 172 GAGCAC G[AT]GC[AT]^C
1288 GAGCTC
BanI 479 GGTACC G^G[CT][GA]CC
1339 GGCACC
BbsI 346 GAAGAC GAAGAC(2/6)
858 GAAGAC
BfaI 493 CTAG C^TAG
1271 CTAG
BglI 32 GCCAGTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
95 GCCCCGAGGGC
BpuAI 346 GAAGAC GAAGAC(2/6)
858 GAAGAC
BsgI 652 CTGCAC GTGCAG(16/14)
496 GTGCAG
BsiHKAI 172 GAGCAC G[AT]GC[AT]^C
1288 GAGCTC
BsmAI 926 GAGAC GTCTC(1/5)
1089 GTCTC
CfoI 77 GCGC GCG^C
729 GCGC
DraII 1052 GGGGCCC [GA]G^G[ACGT]CC[CT]
1053 GGGCCCC
EcoO109I 1052 GGGGCCC [GA]G^G[ACGT]CC[CT]
1053 GGGCCCC
HhaI 77 GCGC GCG^C
729 GCGC
HinP1I 77 GCGC G^CGC
729 GCGC
MaeI 493 CTAG C^TAG
1271 CTAG
MaeIII 386 GTGAC ^GT[ACGT]AC
413 GTCAC
MboII 346 GAAGA GAAGA(8/7)
858 GAAGA
MslI 699 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1065 CATCCAGGTG
SmlI 574 CTCAAG C^T[CT][GA]AG
1213 CTCGAG
Tsp45I 386 GTGAC ^GT[GC]AC
413 GTCAC
Tsp509I 339 AATT ^AATT
559 AATT
XcmI 899 CCAAGAACGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1061 CCAGCATCCAGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BalI BanII BbvI BcnI
BmyI BsaJI BseRI BsiYI BslI
BsoBI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DpnII
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeII MboI MluNI MnlI
MscI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI TaqI
TseI TspRI TthHB8I XmaCI XmaI