Restriction map for
pF1KB8125
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII BamHI BanI BbeI BbrPI
BbvCI BciVI BcnI BglI BglII
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrBI BsrDI BsrFI BsrGI BssSI
BstBI BstXI CelII Cfr10I ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
EcoT22I FseI FspI HaeII HapII
HgaI HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MslI MspA1I
MspI MunI NaeI NarI NciI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI ScaI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 628 GACGTC GACGT^C
AcyI 628 GACGTC G[GA]^CG[CT]C
AflII 864 CTTAAG C^TTAAG
AflIII 768 ACATGT A^C[GA][CT]GT
AlwI 221 GGATC GGATC(4/5)
AlwNI 46 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 379 CCTGAGG CC^T[ACGT]AGG
Asp700I 486 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 204 GAGCTC G[AT]GC[AT]^C
AvaI 9 CTCGGG C^[CT]CG[GA]G
AvrII 820 CCTAGG C^CTAGG
BanII 204 GAGCTC G[GA]GC[CT]^C
BbsI 1198 GAAGAC GAAGAC(2/6)
BcgI 652 CGAGTGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1149 TGATCA T^GATCA
BfrI 864 CTTAAG C^TTAAG
BlnI 820 CCTAGG C^CTAGG
BpuAI 1198 GAAGAC GAAGAC(2/6)
BsaHI 628 GACGTC G[GA]^CG[CT]C
BseRI 640 GAGGAG GAGGAG(10/8)
BsiHKAI 204 GAGCTC G[AT]GC[AT]^C
BsoBI 9 CTCGGG C^[CT]CG[GA]G
BspLU11I 768 ACATGT A^CATGT
BspMI 104 ACCTGC ACCTGC(4/8)
BsrI 66 CCAGT ACTGG(1/-1)
Bst1107I 566 GTATAC GTA^TAC
BstEII 1216 GGTTACC G^GT[ACGT]ACC
BstPI 1216 GGTTACC G^GT[ACGT]ACC
BstYI 221 GGATCT [GA]^GATC[CT]
BstZ17I 566 GTATAC GTA^TAC
Bsu36I 379 CCTGAGG CC^T[ACGT]AGG
DraIII 163 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 379 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1216 GGTTACC G^GT[ACGT]ACC
FauI 126 GCGGG CCCGC(4/6)
FbaI 1149 TGATCA T^GATCA
Hin1I 628 GACGTC G[GA]^CG[CT]C
MaeII 629 ACGT A^CGT
MflI 221 GGATCT [GA]^GATC[CT]
NheI 1299 GCTAGC G^CTAGC
PleI 1099 GAGTC GAGTC(4/5)
PpuMI 1340 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 112 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 204 GAGCTC GAGCT^C
SapI 201 GAAGAGC GCTCTTC(1/4)
SexAI 581 ACCTGGT A^CC[AT]GGT
TaqI 705 TCGA T^CGA
TthHB8I 705 TCGA T^CGA
XhoII 221 GGATCT [GA]^GATC[CT]
XmnI 486 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 566 GTATAC GT^[AC][GT]AC
1255 GTCTAC
AccII 142 CGCG CG^CG
144 CGCG
AvaII 92 GGACC G^G[AT]CC
1341 GGACC
BalI 432 TGGCCA TGG^CCA
1206 TGGCCA
BfaI 821 CTAG C^TAG
1300 CTAG
BmyI 169 GTGCCC G[AGT]GC[ACT]^C
204 GAGCTC
BsgI 136 GTGCAG GTGCAG(16/14)
397 GTGCAG
Bsp1286I 169 GTGCCC G[AGT]GC[ACT]^C
204 GAGCTC
BspHI 257 TCATGA T^CATGA
632 TCATGA
BssHII 141 GCGCGC G^CGCGC
143 GCGCGC
BstAPI 162 GCACGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
399 GCAGTTTTTGC
BstUI 142 CGCG CG^CG
144 CGCG
DpnII 222 GATC ^GATC
1150 GATC
DraII 823 AGGCCCC [GA]G^G[ACGT]CC[CT]
1340 GGGACCC
EcoO109I 823 AGGCCCC [GA]G^G[ACGT]CC[CT]
1340 GGGACCC
MaeI 821 CTAG C^TAG
1300 CTAG
MboI 222 GATC ^GATC
1150 GATC
MluNI 432 TGGCCA TGG^CCA
1206 TGGCCA
MscI 432 TGGCCA TGG^CCA
1206 TGGCCA
MvnI 142 CGCG CG^CG
144 CGCG
NcoI 1203 CCATGG C^CATGG
1346 CCATGG
NdeII 222 GATC ^GATC
1150 GATC
RcaI 257 TCATGA T^CATGA
632 TCATGA
Sau3AI 222 GATC ^GATC
1150 GATC
SfaNI 215 GATGC GCATC(5/9)
662 GCATC
SmlI 342 CTTGAG C^T[CT][GA]AG
864 CTTAAG
SspI 271 AATATT AAT^ATT
454 AATATT
Tsp45I 1016 GTGAC ^GT[GC]AC
1101 GTCAC
XcmI 740 CCATAACACCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1346 CCATGGGAGCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BsaJI BsiYI BslI BsmAI
BsmFI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DsaI EaeI
EarI EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HhaI HinP1I HinfI
HphI ItaI Ksp632I MaeIII MboII
MnlI MseI MvaI MwoI NlaIII
NlaIV NspI RsaI Sau96I ScrFI
StyI TfiI Tru9I TseI Tsp509I
TspRI