Restriction map for
pF1KB8124
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BbvCI BcgI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BsrI BssHII Bst1107I BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoT14I
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI MaeI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI
PaeR7I PflFI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SbfI
ScaI SexAI SfcI SfiI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI TfiI TspRI Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccI 126 GTCGAC GT^[AC][GT]AC
AcyI 361 GGCGCC G[GA]^CG[CT]C
AgeI 475 ACCGGT A^CCGGT
AlwNI 532 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 859 CCTGAGG CC^T[ACGT]AGG
BbeI 361 GGCGCC GGCGC^C
BbsI 865 GAAGAC GAAGAC(2/6)
BciVI 1125 GGATAC GTATCC(6/5)
BpuAI 865 GAAGAC GAAGAC(2/6)
BsaHI 361 GGCGCC G[GA]^CG[CT]C
BsaWI 475 ACCGGT [AT]^CCGG[AT]
BsrBI 456 CCGCTC CCGCTC(-3/-3)
BssSI 525 CTCGTG CACGAG(-5/-1)
BstAPI 152 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1187 TTCGAA TT^CGAA
BstYI 981 GGATCT [GA]^GATC[CT]
Bsu36I 859 CCTGAGG CC^T[ACGT]AGG
DraIII 153 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 859 CCTGAGG CC^T[ACGT]AGG
EcoNI 351 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1073 GATATC GAT^ATC
Hin1I 361 GGCGCC G[GA]^CG[CT]C
HincII 126 GTCGAC GT[CT]^[GA]AC
HindII 126 GTCGAC GT[CT]^[GA]AC
KasI 361 GGCGCC G^GCGCC
MflI 981 GGATCT [GA]^GATC[CT]
NarI 361 GGCGCC GG^CGCC
NlaIII 84 CATG CATG^
NspV 1187 TTCGAA TT^CGAA
PinAI 475 ACCGGT A^CCGGT
PpuMI 644 AGGACCC [GA]G^G[AT]CC[CT]
SacI 507 GAGCTC GAGCT^C
SalI 126 GTCGAC G^TCGAC
SapI 504 GAAGAGC GCTCTTC(1/4)
SfoI 361 GGCGCC GGC^GCC
SfuI 1187 TTCGAA TT^CGAA
SgrAI 599 CGCCGGTG C[GA]^CCGG[CT]G
Tsp45I 472 GTCAC ^GT[GC]AC
XcmI 1130 CCAGAGAGGCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 981 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 1150 AAATTT [GA]^AATT[CT]
1183 GAATTT
AfaI 246 GTAC GT^AC
582 GTAC
ApoI 1150 AAATTT [GA]^AATT[CT]
1183 GAATTT
AspHI 507 GAGCTC G[AT]GC[AT]^C
1157 GTGCTC
BanI 151 GGCACC G^G[CT][GA]CC
361 GGCGCC
BglI 310 GCCTCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
378 GCCGCAGCGGC
BsaAI 94 TACGTG [CT]AC^GT[GA]
439 TACGTG
BsaBI 841 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1069 GATGGATATC
BsgI 139 CTGCAC GTGCAG(16/14)
166 GTGCAG
BsiHKAI 507 GAGCTC G[AT]GC[AT]^C
1157 GTGCTC
BspMI 560 ACCTGC ACCTGC(4/8)
584 ACCTGC
DsaI 365 CCGCGG C^C[GA][CT]GG
656 CCGCGG
EarI 504 GAAGAG CTCTTC(1/4)
706 GAAGAG
HaeII 207 GGCGCT [GA]GCGC^[CT]
361 GGCGCC
HinfI 54 GACTC G^A[ACGT]TC
868 GACTC
Ksp632I 504 GAAGAG CTCTTC(1/4)
706 GAAGAG
KspI 365 CCGCGG CCGC^GG
656 CCGCGG
MamI 841 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1069 GATGGATATC
PleI 54 GACTC GAGTC(4/5)
868 GACTC
RsaI 246 GTAC GT^AC
582 GTAC
SacII 365 CCGCGG CCGC^GG
656 CCGCGG
SfaNI 412 GATGC GCATC(5/9)
756 GATGC
SmlI 553 CTCAAG C^T[CT][GA]AG
625 CTCAAG
Tsp509I 1151 AATT ^AATT
1184 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AccII AciI AluI AlwI ApaI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIV Sau3AI
Sau96I ScrFI SmaI StuI TaqI
Tru9I TseI TthHB8I XmaCI XmaI