Restriction map for
pF1KB8123
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# NO CUT ENZYMES ( 128 enzymes) #
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Acc65I AccIII AclI AflII AflIII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrFI BsrGI
BssHII BstAPI BstBI CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FauI
FbaI FseI FspI HaeII HgaI
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MluI MluNI MroI
MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI Tth111I
TthHB8I Van91I XhoI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 576 GACGTC GACGT^C
AccII 641 CGCG CG^CG
AcyI 576 GACGTC G[GA]^CG[CT]C
AocI 927 CCTGAGG CC^T[ACGT]AGG
AseI 331 ATTAAT AT^TAAT
AsnI 331 ATTAAT AT^TAAT
AvaI 875 CCCGGG C^[CT]CG[GA]G
BanII 933 GGGCTC G[GA]GC[CT]^C
BciVI 1184 GGATAC GTATCC(6/5)
BsaHI 576 GACGTC G[GA]^CG[CT]C
BsmBI 578 CGTCTC CGTCTC(1/5)
BsoBI 875 CCCGGG C^[CT]CG[GA]G
BsrBI 275 GAGCGG CCGCTC(-3/-3)
BsrDI 699 CATTGC GCAATG(2/0)
BssSI 1527 CACGAG CACGAG(-5/-1)
Bst1107I 1542 GTATAC GTA^TAC
BstUI 641 CGCG CG^CG
BstXI 480 CCAGAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1028 AGATCC [GA]^GATC[CT]
BstZ17I 1542 GTATAC GTA^TAC
Bsu36I 927 CCTGAGG CC^T[ACGT]AGG
Cac8I 1513 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 642 GCGC GCG^C
DraI 414 TTTAAA TTT^AAA
DraII 1097 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 571 TGGCCG [CT]^GGCC[GA]
Eco81I 927 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1097 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1202 ATGCAT ATGCA^T
HhaI 642 GCGC GCG^C
Hin1I 576 GACGTC G[GA]^CG[CT]C
HinP1I 642 GCGC G^CGC
HincII 757 GTTGAC GT[CT]^[GA]AC
HindII 757 GTTGAC GT[CT]^[GA]AC
MaeII 577 ACGT A^CGT
MfeI 1238 CAATTG C^AATTG
MflI 1028 AGATCC [GA]^GATC[CT]
MunI 1238 CAATTG C^AATTG
MvnI 641 CGCG CG^CG
NsiI 1202 ATGCAT ATGCA^T
PpuMI 1097 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 331 ATTAAT AT^TAAT
PstI 49 CTGCAG CTGCA^G
SmaI 875 CCCGGG CCC^GGG
SspI 808 AATATT AAT^ATT
TfiI 1365 GAATC G^A[AT]TC
XbaI 1170 TCTAGA T^CTAGA
XhoII 1028 AGATCC [GA]^GATC[CT]
XmaCI 875 CCCGGG C^CCGGG
XmaI 875 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 906 GTAC GT^AC
1104 GTAC
Asp700I 358 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
996 GAAAACATTC
AspHI 422 GAGCAC G[AT]GC[AT]^C
467 GTGCTC
BanI 490 GGCACC G^G[CT][GA]CC
957 GGCACC
BbsI 182 GAAGAC GAAGAC(2/6)
748 GAAGAC
BpuAI 182 GAAGAC GAAGAC(2/6)
748 GAAGAC
BsiHKAI 422 GAGCAC G[AT]GC[AT]^C
467 GTGCTC
BsmFI 977 GGGAC GGGAC(10/14)
1230 GGGAC
BspMI 426 ACCTGC ACCTGC(4/8)
1046 ACCTGC
BstEII 58 GGTAACC G^GT[ACGT]ACC
171 GGTGACC
BstPI 58 GGTAACC G^GT[ACGT]ACC
171 GGTGACC
EcoO65I 58 GGTAACC G^GT[ACGT]ACC
171 GGTGACC
EcoT14I 263 CCAAGG C^C[AT][AT]GG
1454 CCAAGG
HaeIII 572 GGCC GG^CC
924 GGCC
HapII 662 CCGG C^CGG
876 CCGG
HpaII 662 CCGG C^CGG
876 CCGG
MspI 662 CCGG C^CGG
876 CCGG
NspI 463 GCATGT [GA]CATG^[CT]
1204 GCATGT
RsaI 906 GTAC GT^AC
1104 GTAC
SfaNI 307 GATGC GCATC(5/9)
1270 GATGC
SfcI 49 CTGCAG C^T[GA][CT]AG
316 CTACAG
SmlI 588 CTTGAG C^T[CT][GA]AG
1033 CTCAAG
StyI 263 CCAAGG C^C[AT][AT]GG
1454 CCAAGG
XcmI 583 CCAGGCTTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
964 CCAGTGACCACCTGG
XmnI 358 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
996 GAAAACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccI AciI AcsI AluI AlwI
ApoI AvaII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
Tru9I TseI Tsp45I Tsp509I TspRI