Restriction map for
pF1KB8121
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# NO CUT ENZYMES ( 161 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I AlwNI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmFI BsoBI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Cac8I CelII
Cfr10I ClaI CpoI DraI DraII
DrdI EagI Eam1105I EclXI Eco52I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HapII HgaI Hin1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MfeI MluI MroI
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsaI RsrII
SacI SacII SalI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AlwI 142 GATCC GGATC(4/5)
AocI 87 CCTGAGG CC^T[ACGT]AGG
Aor51HI 155 AGCGCT AGC^GCT
AvaII 820 GGACC G^G[AT]CC
BalI 513 TGGCCA TGG^CCA
BanI 295 GGTGCC G^G[CT][GA]CC
BanII 25 GAGCCC G[GA]GC[CT]^C
BbvCI 36 GCTGAGG CCTCAGC(-5/-2)
BfaI 560 CTAG C^TAG
BglI 860 GCCCCTATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 399 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 648 CGGTCG CG[GA][CT]^CG
BsmI 216 GAATGC GAATGC(1/-1)
BspHI 407 TCATGA T^CATGA
BsrDI 105 GCAATG GCAATG(2/0)
BstYI 141 AGATCC [GA]^GATC[CT]
Bsu36I 87 CCTGAGG CC^T[ACGT]AGG
CfoI 156 GCGC GCG^C
DpnII 142 GATC ^GATC
DraIII 229 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 513 TGGCCA [CT]^GGCC[GA]
Eco47III 155 AGCGCT AGC^GCT
Eco81I 87 CCTGAGG CC^T[ACGT]AGG
EcoT14I 903 CCAAGG C^C[AT][AT]GG
HaeII 155 AGCGCT [GA]GCGC^[CT]
HhaI 156 GCGC GCG^C
HinP1I 156 GCGC G^CGC
HphI 357 TCACC GGTGA(8/7)
MaeI 560 CTAG C^TAG
MamI 399 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 142 GATC ^GATC
MflI 141 AGATCC [GA]^GATC[CT]
MluNI 513 TGGCCA TGG^CCA
MscI 513 TGGCCA TGG^CCA
MslI 517 CACAACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 34 CAGCTG C[AC]G^C[GT]G
NdeI 182 CATATG CA^TATG
NdeII 142 GATC ^GATC
PvuII 34 CAGCTG CAG^CTG
RcaI 407 TCATGA T^CATGA
SapI 45 GCTCTTC GCTCTTC(1/4)
Sau3AI 142 GATC ^GATC
SexAI 291 ACCAGGT A^CC[AT]GGT
SfaNI 205 GATGC GCATC(5/9)
StyI 903 CCAAGG C^C[AT][AT]GG
TaqI 585 TCGA T^CGA
TfiI 280 GATTC G^A[AT]TC
Tsp45I 178 GTCAC ^GT[GC]AC
TthHB8I 585 TCGA T^CGA
XhoII 141 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 869 GCGG CCGC(-3/-1)
917 GCGG
AcsI 813 AAATTT [GA]^AATT[CT]
837 AAATTT
ApoI 813 AAATTT [GA]^AATT[CT]
837 AAATTT
Asp700I 42 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
587 GAAGTGGTTC
BbsI 340 GAAGAC GAAGAC(2/6)
807 GTCTTC
BmyI 25 GAGCCC G[AGT]GC[ACT]^C
296 GTGCCC
BpmI 159 CTCCAG CTGGAG(16/14)
270 CTCCAG
BpuAI 340 GAAGAC GAAGAC(2/6)
807 GTCTTC
BseRI 90 GAGGAG GAGGAG(10/8)
196 GAGGAG
BsiYI 823 CCCATGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
862 CCCTATGGCGG
BslI 823 CCCATGAAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
862 CCCTATGGCGG
BsmAI 15 GTCTC GTCTC(1/5)
268 GTCTC
Bsp1286I 25 GAGCCC G[AGT]GC[ACT]^C
296 GTGCCC
BssKI 161 CCAGG ^CC[ACGT]GG
292 CCAGG
BstNI 161 CCAGG CC^[AT]GG
292 CCAGG
Cfr13I 820 GGACC G^G[ACGT]CC
859 GGCCC
DsaI 473 CCGTGG C^C[GA][CT]GG
693 CCGTGG
EarI 46 CTCTTC CTCTTC(1/4)
242 GAAGAG
EcoRII 161 CCAGG ^CC[AT]GG
292 CCAGG
Ksp632I 46 CTCTTC CTCTTC(1/4)
242 GAAGAG
MaeIII 178 GTCAC ^GT[ACGT]AC
451 GTAAC
MseI 304 TTAA T^TAA
335 TTAA
MvaI 161 CCAGG CC^[AT]GG
292 CCAGG
PleI 469 GACTC GAGTC(4/5)
13 GAGTC
Sau96I 820 GGACC G^G[ACGT]CC
859 GGCCC
ScrFI 161 CCAGG CC^[ACGT]GG
292 CCAGG
Tru9I 304 TTAA T^TAA
335 TTAA
XcmI 501 CCATACTGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
903 CCAAGGTGGCTATGG
XmnI 42 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
587 GAAGTGGTTC
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AluI BbvI BsaJI BstF5I DdeI
Fnu4HI FokI HaeIII HinfI ItaI
MboII MnlI MwoI NlaIII NlaIV
TseI Tsp509I TspRI