Restriction map for
pF1KB8120
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvrII BamHI
BbeI BbsI BbvCI BcgI BciVI
BfrI BglII BlnI BpuAI BsaBI
BsaHI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FseI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SwaI Tsp509I
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccIII 1250 TCCGGA T^CCGGA
AflIII 444 ACGCGT A^C[GA][CT]GT
AocI 467 CCTCAGG CC^T[ACGT]AGG
AvaI 208 CCCGAG C^[CT]CG[GA]G
AviII 905 TGCGCA TGC^GCA
BfaI 589 CTAG C^TAG
BlpI 717 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 717 GCTGAGC GC^T[ACGT]AGC
BseAI 1250 TCCGGA T^CCGGA
BsmI 139 GAATGC GAATGC(1/-1)
BsoBI 208 CCCGAG C^[CT]CG[GA]G
BspEI 1250 TCCGGA T^CCGGA
BspMI 33 GCAGGT ACCTGC(4/8)
BsrDI 284 GCAATG GCAATG(2/0)
BsrFI 1118 ACCGGC [GA]^CCGG[CT]
BssSI 1005 CTCGTG CACGAG(-5/-1)
BstAPI 486 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 467 CCTCAGG CC^T[ACGT]AGG
CelII 717 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1118 ACCGGC [GA]^CCGG[CT]
Eco81I 467 CCTCAGG CC^T[ACGT]AGG
EcoT14I 725 CCAAGG C^C[AT][AT]GG
EcoT22I 583 ATGCAT ATGCA^T
FspI 905 TGCGCA TGC^GCA
MaeI 589 CTAG C^TAG
MluI 444 ACGCGT A^CGCGT
MroI 1250 TCCGGA T^CCGGA
MseI 203 TTAA T^TAA
NsiI 583 ATGCAT ATGCA^T
PflMI 126 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1226 AGGTCCC [GA]G^G[AT]CC[CT]
SacI 745 GAGCTC GAGCT^C
ScaI 218 AGTACT AGT^ACT
SexAI 412 ACCTGGT A^CC[AT]GGT
SspI 199 AATATT AAT^ATT
StuI 851 AGGCCT AGG^CCT
StyI 725 CCAAGG C^C[AT][AT]GG
TaqI 1028 TCGA T^CGA
TfiI 862 GATTC G^A[AT]TC
Tru9I 203 TTAA T^TAA
TthHB8I 1028 TCGA T^CGA
Van91I 126 CCATGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 219 GTAC GT^AC
273 GTAC
Alw44I 493 GTGCAC G^TGCAC
950 GTGCAC
ApaLI 493 GTGCAC G^TGCAC
950 GTGCAC
BanI 674 GGCACC G^G[CT][GA]CC
784 GGTGCC
BbrPI 496 CACGTG CAC^GTG
901 CACGTG
BclI 1055 TGATCA T^GATCA
1145 TGATCA
BcnI 330 CCGGG CC^[GC]GG
1356 CCGGG
BglI 356 GCCAGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
844 GCCCTGCAGGC
BsaAI 496 CACGTG [CT]AC^GT[GA]
901 CACGTG
BsaI 385 GAGACC GGTCTC(1/5)
344 GGTCTC
BsaWI 107 ACCGGA [AT]^CCGG[AT]
1250 TCCGGA
BspHI 224 TCATGA T^CATGA
1052 TCATGA
BsrI 1338 ACTGG ACTGG(1/-1)
270 CCAGT
BstXI 29 CCATGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1088 CCAACTACCTGG
EarI 1399 CTCTTC CTCTTC(1/4)
931 GAAGAG
FbaI 1055 TGATCA T^GATCA
1145 TGATCA
HgaI 443 GACGC GACGC(5/10)
446 GCGTC
Ksp632I 1399 CTCTTC CTCTTC(1/4)
931 GAAGAG
MslI 30 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
576 CACAGAGATG
MspA1I 487 CAGCTG C[AC]G^C[GT]G
831 CAGCTG
NciI 330 CCGGG CC^[GC]GG
1356 CCGGG
PmaCI 496 CACGTG CAC^GTG
901 CACGTG
PmlI 496 CACGTG CAC^GTG
901 CACGTG
PvuII 487 CAGCTG CAG^CTG
831 CAGCTG
RcaI 224 TCATGA T^CATGA
1052 TCATGA
RsaI 219 GTAC GT^AC
273 GTAC
SbfI 846 CCTGCAGG CCTGCA^GG
1233 CCTGCAGG
SmlI 94 CTCAAG C^T[CT][GA]AG
178 CTCAAG
Sse8387I 846 CCTGCAGG CCTGCA^GG
1233 CCTGCAGG
TspRI 171 CAGTG CA[GC]TG[ACGT][ACGT]^
968 CACTG
XcmI 270 CCAGTACGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
725 CCAAGGACATAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AccII AciI AluI AlwI AlwNI
ApaI AspHI AvaII BalI BanII
BbvI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp45I XhoII