Restriction map for
pF1KB8116
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI KspI
MaeIII MamI MfeI MluI MroI
MseI MslI MspI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tsp45I Tsp509I Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 126 GTCTAC GT^[AC][GT]AC
AfaI 404 GTAC GT^AC
AocI 352 CCTGAGG CC^T[ACGT]AGG
ApaI 544 GGGCCC GGGCC^C
AspHI 481 GTGCTC G[AT]GC[AT]^C
AvaII 514 GGTCC G^G[AT]CC
BanI 62 GGTGCC G^G[CT][GA]CC
BbsI 658 GTCTTC GAAGAC(2/6)
BpuAI 658 GTCTTC GAAGAC(2/6)
BseRI 367 CTCCTC GAGGAG(10/8)
BsgI 565 CTGCAC GTGCAG(16/14)
BsiHKAI 481 GTGCTC G[AT]GC[AT]^C
BsmAI 253 GAGAC GTCTC(1/5)
BspMI 230 ACCTGC ACCTGC(4/8)
BsrDI 497 GCAATG GCAATG(2/0)
BstF5I 180 GGATG GGATG(2/0)
BstYI 423 GGATCT [GA]^GATC[CT]
Bsu36I 352 CCTGAGG CC^T[ACGT]AGG
DsaI 114 CCATGG C^C[GA][CT]GG
Eco81I 352 CCTGAGG CC^T[ACGT]AGG
EcoT14I 114 CCATGG C^C[AT][AT]GG
FauI 556 CCCGC CCCGC(4/6)
FokI 180 GGATG GGATG(9/13)
HphI 468 GGTGA GGTGA(8/7)
MaeII 464 ACGT A^CGT
MflI 423 GGATCT [GA]^GATC[CT]
NcoI 114 CCATGG C^CATGG
NspI 202 GCATGC [GA]CATG^[CT]
PflMI 603 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 513 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 445 CAGCTG CAG^CTG
RsaI 404 GTAC GT^AC
SapI 748 GCTCTTC GCTCTTC(1/4)
SphI 202 GCATGC GCATG^C
StyI 114 CCATGG C^C[AT][AT]GG
TaqI 216 TCGA T^CGA
TthHB8I 216 TCGA T^CGA
Van91I 603 CCAGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 423 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 557 CCGC CCGC(-3/-1)
531 GCGG
AlwI 423 GGATC GGATC(4/5)
720 GGATC
AlwNI 529 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
644 CAGAGGCTG
BalI 111 TGGCCA TGG^CCA
538 TGGCCA
BanII 119 GGGCTC G[GA]GC[CT]^C
544 GGGCCC
BfaI 395 CTAG C^TAG
574 CTAG
DpnII 424 GATC ^GATC
721 GATC
EaeI 111 TGGCCA [CT]^GGCC[GA]
538 TGGCCA
EarI 749 CTCTTC CTCTTC(1/4)
703 GAAGAG
Ksp632I 749 CTCTTC CTCTTC(1/4)
703 GAAGAG
MaeI 395 CTAG C^TAG
574 CTAG
MboI 424 GATC ^GATC
721 GATC
MluNI 111 TGGCCA TGG^CCA
538 TGGCCA
MscI 111 TGGCCA TGG^CCA
538 TGGCCA
MspA1I 445 CAGCTG C[AC]G^C[GT]G
529 CAGCGG
NdeII 424 GATC ^GATC
721 GATC
NlaIII 115 CATG CATG^
203 CATG
Sau3AI 424 GATC ^GATC
721 GATC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstNI Cac8I Cfr13I DdeI DraII
EcoO109I EcoRII Fnu4HI HaeIII ItaI
MboII MnlI MvaI MwoI NlaIV
PstI Sau96I ScrFI SfaNI SfcI
TseI TspRI