Restriction map for
pF1KB8110
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AviII BalI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI Sse8387I
SspBI SspI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 135 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 506 GGGCCC GGGCC^C
AvrII 174 CCTAGG C^CTAGG
BamHI 424 GGATCC G^GATCC
BbvCI 532 GCTGAGG CCTCAGC(-5/-2)
BglI 131 GCCACAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 174 CCTAGG C^CTAGG
BlpI 390 GCTGAGC GC^T[ACGT]AGC
BpmI 286 CTGGAG CTGGAG(16/14)
Bpu1102I 390 GCTGAGC GC^T[ACGT]AGC
BseRI 304 GAGGAG GAGGAG(10/8)
BstXI 35 CCATGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 390 GCTGAGC GC^T[ACGT]AGC
EarI 523 GAAGAG CTCTTC(1/4)
EcoT14I 174 CCTAGG C^C[AT][AT]GG
FauI 473 GCGGG CCCGC(4/6)
Ksp632I 523 GAAGAG CTCTTC(1/4)
MspA1I 135 CAGCGG C[AC]G^C[GT]G
NspI 251 GCATGC [GA]CATG^[CT]
PflMI 120 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SmaI 294 CCCGGG CCC^GGG
SphI 251 GCATGC GCATG^C
StuI 359 AGGCCT AGG^CCT
StyI 174 CCTAGG C^C[AT][AT]GG
Van91I 120 CCAGATCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 294 CCCGGG C^CCGGG
XmaI 294 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AvaI 294 CCCGGG C^[CT]CG[GA]G
490 CCCGAG
AvaII 9 GGTCC G^G[AT]CC
495 GGACC
BanI 309 GGTGCC G^G[CT][GA]CC
436 GGCACC
BanII 487 GAGCCC G[GA]GC[CT]^C
506 GGGCCC
BbvI 46 GCTGC GCAGC(8/12)
353 GCTGC
BfaI 175 CTAG C^TAG
377 CTAG
BsgI 343 CTGCAC GTGCAG(16/14)
403 GTGCAG
BsmFI 199 GTCCC GGGAC(10/14)
228 GTCCC
BsoBI 294 CCCGGG C^[CT]CG[GA]G
490 CCCGAG
BsrI 42 ACTGG ACTGG(1/-1)
261 CCAGT
BssSI 301 CACGAG CACGAG(-5/-1)
382 CACGAG
BstYI 338 AGATCC [GA]^GATC[CT]
424 GGATCC
HinfI 226 GAGTC G^A[ACGT]TC
244 GACTC
HphI 116 TCACC GGTGA(8/7)
447 TCACC
MaeI 175 CTAG C^TAG
377 CTAG
MboII 336 GAAGA GAAGA(8/7)
523 GAAGA
MflI 338 AGATCC [GA]^GATC[CT]
424 GGATCC
MwoI 131 GCCACAGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
356 GCCAGGCCTGC
PleI 244 GACTC GAGTC(4/5)
226 GAGTC
PpuMI 8 GGGTCCT [GA]G^G[AT]CC[CT]
494 AGGACCC
SfcI 192 CTACAG C^T[GA][CT]AG
348 CTGTAG
TseI 46 GCTGC G^C[AT]GC
353 GCTGC
XhoII 338 AGATCC [GA]^GATC[CT]
424 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AlwI BcnI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HgaI HpaII
ItaI MaeIII MboI MnlI MspI
MvaI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI Tsp45I TspRI
XcmI