Restriction map for
pF1KB8106
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AvaI AvaII
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII
BstNI BstPI BstUI BstXI Bsu36I
CelII Cfr10I Cfr13I ClaI CpoI
DraI DraII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRII EcoRV EcoT14I EcoT22I FauI
FbaI FseI FspI HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MfeI MluI
MluNI MroI MscI MslI MspA1I
MunI MvaI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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Asp700I 138 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 335 GAGCAC G[AT]GC[AT]^C
AspI 474 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 312 GCTGAGG CCTCAGC(-5/-2)
BcnI 432 CCGGG CC^[GC]GG
BfaI 403 CTAG C^TAG
BglII 219 AGATCT A^GATCT
BmyI 335 GAGCAC G[AGT]GC[ACT]^C
BpmI 343 CTGGAG CTGGAG(16/14)
BsaBI 64 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 335 GAGCAC G[AT]GC[AT]^C
BsmFI 457 GTCCC GGGAC(10/14)
Bsp1286I 335 GAGCAC G[AGT]GC[ACT]^C
BspMI 420 GCAGGT ACCTGC(4/8)
BsrI 107 CCAGT ACTGG(1/-1)
BssKI 432 CCGGG ^CC[ACGT]GG
BstYI 219 AGATCT [GA]^GATC[CT]
Cac8I 333 GCGAGC GC[ACGT]^[ACGT]GC
CfoI 7 GCGC GCG^C
DraIII 430 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 289 CTCTTC CTCTTC(1/4)
HaeII 6 GGCGCT [GA]GCGC^[CT]
HapII 432 CCGG C^CGG
HhaI 7 GCGC GCG^C
HinP1I 7 GCGC G^CGC
HpaII 432 CCGG C^CGG
Ksp632I 289 CTCTTC CTCTTC(1/4)
MaeI 403 CTAG C^TAG
MaeIII 519 GTCAC ^GT[ACGT]AC
MamI 64 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 219 AGATCT [GA]^GATC[CT]
MseI 442 TTAA T^TAA
MspI 432 CCGG C^CGG
MwoI 333 GCGAGCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 432 CCGGG CC^[GC]GG
NlaIII 351 CATG CATG^
NlaIV 18 GGAACC GG[ACGT]^[ACGT]CC
PflFI 474 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 417 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 295 GACTC GAGTC(4/5)
PstI 490 CTGCAG CTGCA^G
ScaI 86 AGTACT AGT^ACT
ScrFI 432 CCGGG CC^[ACGT]GG
SfcI 490 CTGCAG C^T[GA][CT]AG
TfiI 51 GATTC G^A[AT]TC
Tru9I 442 TTAA T^TAA
Tsp45I 519 GTCAC ^GT[GC]AC
Tth111I 474 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 417 CCAGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 44 CCATCCTGATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 219 AGATCT [GA]^GATC[CT]
XmnI 138 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AccI 30 GTATAC GT^[AC][GT]AC
202 GTATAC
AfaI 87 GTAC GT^AC
427 GTAC
AluI 244 AGCT AG^CT
260 AGCT
AlwI 399 GATCC GGATC(4/5)
412 GATCC
AlwNI 307 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
484 CAGGTGCTG
BbvI 242 GCAGC GCAGC(8/12)
489 GCTGC
BsmAI 287 GTCTC GTCTC(1/5)
480 GTCTC
Bst1107I 30 GTATAC GTA^TAC
202 GTATAC
BstF5I 45 CATCC GGATG(2/0)
375 GGATG
BstZ17I 30 GTATAC GTA^TAC
202 GTATAC
DdeI 313 CTGAG C^T[ACGT]AG
482 CTCAG
Fnu4HI 242 GCAGC GC^[ACGT]GC
489 GCTGC
FokI 45 CATCC GGATG(9/13)
375 GGATG
HaeIII 126 GGCC GG^CC
190 GGCC
HinfI 51 GATTC G^A[ACGT]TC
295 GACTC
HphI 396 GGTGA GGTGA(8/7)
448 GGTGA
ItaI 242 GCAGC GC^[ACGT]GC
489 GCTGC
RsaI 87 GTAC GT^AC
427 GTAC
SfaNI 264 GATGC GCATC(5/9)
309 GATGC
StuI 125 AGGCCT AGG^CCT
189 AGGCCT
TaqI 72 TCGA T^CGA
293 TCGA
TseI 242 GCAGC G^C[AT]GC
489 GCTGC
Tsp509I 174 AATT ^AATT
381 AATT
TthHB8I 72 TCGA T^CGA
293 TCGA
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# 3 OR MORE CUT ENZYMES ( 9 enzymes) #
#########################################
BsiYI BslI DpnII MboI MboII
MnlI NdeII Sau3AI TspRI