Restriction map for
pF1KB8103
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# NO CUT ENZYMES ( 191 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AvaII
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BlnI BpmI BpuAI
BsaAI BsaBI BsaI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI Eam1105I EarI Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FokI FseI
FspI HgaI HincII HindII HindIII
HinfI HpaI HphI KpnI Ksp632I
KspI MaeI MaeIII MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NlaIII
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI ScaI SexAI SfaNI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI SspBI SspI StuI StyI
SwaI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcyI 109 GGCGCC G[GA]^CG[CT]C
BanII 36 GAGCCC G[GA]GC[CT]^C
BbeI 109 GGCGCC GGCGC^C
BbvI 12 GCTGC GCAGC(8/12)
BcnI 113 CCGGG CC^[GC]GG
BglII 68 AGATCT A^GATCT
BlpI 33 GCTGAGC GC^T[ACGT]AGC
BmyI 36 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 33 GCTGAGC GC^T[ACGT]AGC
BsaHI 109 GGCGCC G[GA]^CG[CT]C
Bsp1286I 36 GAGCCC G[AGT]GC[ACT]^C
BstNI 226 CCTGG CC^[AT]GG
BstYI 68 AGATCT [GA]^GATC[CT]
CelII 33 GCTGAGC GC^T[ACGT]AGC
CfoI 110 GCGC GCG^C
DdeI 34 CTGAG C^T[ACGT]AG
EcoRII 226 CCTGG ^CC[AT]GG
FauI 162 GCGGG CCCGC(4/6)
HaeII 109 GGCGCC [GA]GCGC^[CT]
HhaI 110 GCGC GCG^C
Hin1I 109 GGCGCC G[GA]^CG[CT]C
HinP1I 110 GCGC G^CGC
KasI 109 GGCGCC G^GCGCC
MflI 68 AGATCT [GA]^GATC[CT]
MvaI 226 CCTGG CC^[AT]GG
NarI 109 GGCGCC GG^CGCC
NciI 113 CCGGG CC^[GC]GG
NotI 126 GCGGCCGC GC^GGCCGC
PstI 85 CTGCAG CTGCA^G
SbfI 84 CCTGCAGG CCTGCA^GG
SfcI 85 CTGCAG C^T[GA][CT]AG
SfiI 142 GGCCAGATCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 109 GGCGCC GGC^GCC
Sse8387I 84 CCTGCAGG CCTGCA^GG
TseI 12 GCTGC G^C[AT]GC
XhoII 68 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AluI 32 AGCT AG^CT
137 AGCT
BanI 109 GGCGCC G^G[CT][GA]CC
219 GGCACC
BglI 38 GCCCCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
143 GCCAGATCGGC
BsiEI 127 CGGCCG CG[GA][CT]^CG
150 CGGCCG
BsiYI 81 CCGCCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
133 CCCAAGCTGGG
BslI 81 CCGCCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
133 CCCAAGCTGGG
BssKI 113 CCGGG ^CC[ACGT]GG
226 CCTGG
Cac8I 83 GCCTGC GC[ACGT]^[ACGT]GC
158 GCAAGC
Cfr13I 63 GGGCC G^G[ACGT]CC
141 GGGCC
DpnII 69 GATC ^GATC
147 GATC
EaeI 127 CGGCCG [CT]^GGCC[GA]
150 CGGCCG
EagI 127 CGGCCG C^GGCCG
150 CGGCCG
EclXI 127 CGGCCG C^GGCCG
150 CGGCCG
Eco52I 127 CGGCCG C^GGCCG
150 CGGCCG
HapII 113 CCGG C^CGG
153 CCGG
HpaII 113 CCGG C^CGG
153 CCGG
MaeII 59 ACGT A^CGT
194 ACGT
MboI 69 GATC ^GATC
147 GATC
MnlI 223 CCTC CCTC(7/6)
7 GAGG
MspI 113 CCGG C^CGG
153 CCGG
NdeII 69 GATC ^GATC
147 GATC
NlaIV 109 GGCGCC GG[ACGT]^[ACGT]CC
219 GGCACC
Sau3AI 69 GATC ^GATC
147 GATC
Sau96I 63 GGGCC G^G[ACGT]CC
141 GGGCC
ScrFI 113 CCGGG CC^[ACGT]GG
226 CCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
AciI Fnu4HI HaeIII ItaI MboII
MwoI