Restriction map for
pF1KB8102
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT14I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MfeI MflI MluI MroI
MunI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StyI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AflIII 325 ACACGT A^C[GA][CT]GT
AlwI 1149 GATCC GGATC(4/5)
AlwNI 231 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 978 AGCGCT AGC^GCT
BbeI 808 GGCGCC GGCGC^C
BfaI 887 CTAG C^TAG
BglI 1100 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 826 CTGGAG CTGGAG(16/14)
BsaBI 1149 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 941 GAGACC GGTCTC(1/5)
BsgI 832 CTGCAC GTGCAG(16/14)
BsmI 876 GAATGC GAATGC(1/-1)
BspHI 344 TCATGA T^CATGA
BsrBI 672 GAGCGG CCGCTC(-3/-3)
BsrDI 85 GCAATG GCAATG(2/0)
BsrFI 890 GCCGGT [GA]^CCGG[CT]
BstXI 1154 CCATCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 890 GCCGGT [GA]^CCGG[CT]
DpnII 1149 GATC ^GATC
DraI 952 TTTAAA TTT^AAA
DraII 685 GGGACCT [GA]G^G[ACGT]CC[CT]
Eco47III 978 AGCGCT AGC^GCT
EcoNI 728 CCTATGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 685 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 67 ATGCAT ATGCA^T
KasI 808 GGCGCC G^GCGCC
MaeI 887 CTAG C^TAG
MamI 1149 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 1149 GATC ^GATC
MseI 953 TTAA T^TAA
NarI 808 GGCGCC GG^CGCC
NdeII 1149 GATC ^GATC
NsiI 67 ATGCAT ATGCA^T
NspI 1142 GCATGC [GA]CATG^[CT]
PpuMI 685 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 259 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 715 CAGCTG CAG^CTG
RcaI 344 TCATGA T^CATGA
SacI 393 GAGCTC GAGCT^C
SapI 858 GCTCTTC GCTCTTC(1/4)
Sau3AI 1149 GATC ^GATC
SexAI 815 ACCTGGT A^CC[AT]GGT
SfoI 808 GGCGCC GGC^GCC
SphI 1142 GCATGC GCATG^C
StuI 583 AGGCCT AGG^CCT
Tru9I 953 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 117 CGCG CG^CG
1017 CGCG
AcyI 808 GGCGCC G[GA]^CG[CT]C
934 GACGCC
AfaI 531 GTAC GT^AC
663 GTAC
BanII 393 GAGCTC G[GA]GC[CT]^C
645 GAGCCC
BcnI 10 CCCGG CC^[GC]GG
20 CCCGG
BsaHI 808 GGCGCC G[GA]^CG[CT]C
934 GACGCC
BsmFI 112 GGGAC GGGAC(10/14)
685 GGGAC
BspMI 197 ACCTGC ACCTGC(4/8)
688 ACCTGC
BssSI 312 CACGAG CACGAG(-5/-1)
268 CTCGTG
BstF5I 522 CATCC GGATG(2/0)
872 GGATG
BstUI 117 CGCG CG^CG
1017 CGCG
DdeI 150 CTCAG C^T[ACGT]AG
179 CTAAG
DraIII 566 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
814 CACCTGGTG
FauI 797 GCGGG CCCGC(4/6)
1177 GCGGG
FokI 522 CATCC GGATG(9/13)
872 GGATG
Hin1I 808 GGCGCC G[GA]^CG[CT]C
934 GACGCC
MspA1I 715 CAGCTG C[AC]G^C[GT]G
795 CAGCGG
MvnI 117 CGCG CG^CG
1017 CGCG
NciI 10 CCCGG CC^[GC]GG
20 CCCGG
PleI 822 GACTC GAGTC(4/5)
1227 GAGTC
PstI 712 CTGCAG CTGCA^G
982 CTGCAG
RsaI 531 GTAC GT^AC
663 GTAC
SfaNI 208 GATGC GCATC(5/9)
514 GATGC
SfcI 712 CTGCAG C^T[GA][CT]AG
982 CTGCAG
TfiI 418 GAATC G^A[AT]TC
908 GAATC
TspRI 152 CAGTG CA[GC]TG[ACGT][ACGT]^
215 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AciI AluI AspHI AvaII BalI
BanI BbvI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I EaeI EarI EcoRII Fnu4HI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboII MluNI
MnlI MscI MslI MspI MvaI
MwoI NlaIII NlaIV Sau96I ScrFI
TseI Tsp45I Tsp509I