Restriction map for
pF1KB7795
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AhdI Alw44I AlwI
AocI ApaLI ApoI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbeI BciVI BclI BfrI BglII
BlnI BsaBI BsaHI BsaI BseAI
BsgI BsiWI BspDI BspEI BspHI
BspLU11I BspMI BsrDI Bst1107I BstBI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DsaI Eam1105I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MflI MluI MroI MslI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI SwaI TfiI Tsp509I
XbaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 259 GGTACC G^GTACC
AflIII 1219 ACGTGT A^C[GA][CT]GT
AgeI 546 ACCGGT A^CCGGT
Aor51HI 842 AGCGCT AGC^GCT
ApaI 1050 GGGCCC GGGCC^C
Asp718I 259 GGTACC G^GTACC
AspI 129 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 114 TGGCCA TGG^CCA
BbrPI 1218 CACGTG CAC^GTG
BbsI 144 GTCTTC GAAGAC(2/6)
BlpI 1080 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1080 GCTCAGC GC^T[ACGT]AGC
BpuAI 144 GTCTTC GAAGAC(2/6)
BsaAI 1218 CACGTG [CT]AC^GT[GA]
BsaWI 546 ACCGGT [AT]^CCGG[AT]
BseRI 237 CTCCTC GAGGAG(10/8)
BsiEI 1239 CGGCCG CG[GA][CT]^CG
BsmBI 425 CGTCTC CGTCTC(1/5)
BsmI 1120 GAATGC GAATGC(1/-1)
Bsp1407I 176 TGTACA T^GTACA
BsrBI 1309 CCGCTC CCGCTC(-3/-3)
BsrGI 176 TGTACA T^GTACA
BstEII 894 GGTGACC G^GT[ACGT]ACC
BstPI 894 GGTGACC G^GT[ACGT]ACC
BstXI 773 CCACCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1080 GCTCAGC GC^T[ACGT]AGC
DpnII 280 GATC ^GATC
DrdI 1102 GACTGTTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1239 CGGCCG C^GGCCG
EclXI 1239 CGGCCG C^GGCCG
Eco47III 842 AGCGCT AGC^GCT
Eco52I 1239 CGGCCG C^GGCCG
EcoNI 435 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 894 GGTGACC G^GT[ACGT]ACC
HinfI 142 GAGTC G^A[ACGT]TC
KpnI 259 GGTACC GGTAC^C
MaeII 1219 ACGT A^CGT
MboI 280 GATC ^GATC
MluNI 114 TGGCCA TGG^CCA
MscI 114 TGGCCA TGG^CCA
MseI 299 TTAA T^TAA
NaeI 1339 GCCGGC GCC^GGC
NdeII 280 GATC ^GATC
NgoMIV 1339 GCCGGC G^CCGGC
PflFI 129 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 546 ACCGGT A^CCGGT
PleI 142 GAGTC GAGTC(4/5)
PmaCI 1218 CACGTG CAC^GTG
PmlI 1218 CACGTG CAC^GTG
PvuII 1018 CAGCTG CAG^CTG
SacI 555 GAGCTC GAGCT^C
SapI 1374 GCTCTTC GCTCTTC(1/4)
Sau3AI 280 GATC ^GATC
SfaNI 13 GATGC GCATC(5/9)
SgrAI 545 CACCGGTG C[GA]^CCGG[CT]G
SspBI 176 TGTACA T^GTACA
StuI 675 AGGCCT AGG^CCT
Tru9I 299 TTAA T^TAA
TspRI 913 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 129 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 177 GTAC GT^AC
260 GTAC
AlwNI 131 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
524 CAGCCTCTG
Asp700I 1182 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1371 GAAGCTCTTC
BbvCI 455 CCTCAGC CCTCAGC(-5/-2)
642 CCTCAGC
BcgI 791 GCAGCTTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1145 GCAGCTTTTTCG
BglI 217 GCCCCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
677 GCCTCTGTGGC
BpmI 31 CTGGAG CTGGAG(16/14)
466 CTGGAG
BssHII 1085 GCGCGC G^CGCGC
1335 GCGCGC
BssSI 976 CACGAG CACGAG(-5/-1)
1159 CACGAG
EaeI 114 TGGCCA [CT]^GGCC[GA]
1239 CGGCCG
EarI 240 CTCTTC CTCTTC(1/4)
1375 CTCTTC
HgaI 1199 GACGC GACGC(5/10)
938 GCGTC
Ksp632I 240 CTCTTC CTCTTC(1/4)
1375 CTCTTC
NspI 50 ACATGC [GA]CATG^[CT]
1288 ACATGC
PpuMI 5 AGGACCT [GA]G^G[AT]CC[CT]
1321 GGGTCCT
RsaI 177 GTAC GT^AC
260 GTAC
TaqI 800 TCGA T^CGA
1154 TCGA
TthHB8I 800 TCGA T^CGA
1154 TCGA
XcmI 245 CCAGCGGTGTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
461 CCACTCTGGAGCTGG
XmnI 1182 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1371 GAAGCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccII AciI AluI AspHI AvaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeI MaeIII MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV PflMI
PstI Sau96I ScrFI SfcI StyI
TseI Tsp45I Van91I