Restriction map for
pF1KB7755
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# NO CUT ENZYMES ( 120 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwI
AocI ApaLI ApoI AseI AsnI
Asp718I AspI AviII AvrII BalI
BamHI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglII BlnI
BpuAI BsaBI BsaI BsaWI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
BsrDI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DpnII DraI DraIII
DrdI Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NdeI NdeII NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI Sau3AI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI TfiI Tru9I
Tsp509I Tth111I XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 841 GACGTC GACGT^C
AhdI 208 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 185 AGCGCT AGC^GCT
ApaI 947 GGGCCC GGGCC^C
AscI 345 GGCGCGCC GG^CGCGCC
Asp700I 318 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 208 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 70 CACGTG CAC^GTG
BsaAI 70 CACGTG [CT]AC^GT[GA]
BseRI 457 GAGGAG GAGGAG(10/8)
BsmI 322 GCATTC GAATGC(1/-1)
BsrBI 21 CCGCTC CCGCTC(-3/-3)
BssSI 847 CACGAG CACGAG(-5/-1)
BstF5I 201 CATCC GGATG(2/0)
Eam1105I 208 GACGCCGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 528 GAAGAG CTCTTC(1/4)
Eco47III 185 AGCGCT AGC^GCT
FokI 201 CATCC GGATG(9/13)
HphI 1249 TCACC GGTGA(8/7)
Ksp632I 528 GAAGAG CTCTTC(1/4)
MaeIII 1298 GTCAC ^GT[ACGT]AC
MboII 528 GAAGA GAAGA(8/7)
NspI 300 GCATGT [GA]CATG^[CT]
PflMI 1295 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 70 CACGTG CAC^GTG
PmlI 70 CACGTG CAC^GTG
PshAI 238 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 528 GAAGAGC GCTCTTC(1/4)
SbfI 1155 CCTGCAGG CCTGCA^GG
SfaNI 200 GCATC GCATC(5/9)
SmaI 155 CCCGGG CCC^GGG
SmlI 226 CTCAAG C^T[CT][GA]AG
Sse8387I 1155 CCTGCAGG CCTGCA^GG
Tsp45I 1298 GTCAC ^GT[GC]AC
TspRI 1300 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 1295 CCAGTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 62 CCATGTCGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 155 CCCGGG C^CCGGG
XmaI 155 CCCGGG C^CCGGG
XmnI 318 GAACGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AlwNI 859 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1296 CAGTCACTG
BsgI 379 CTGCAC GTGCAG(16/14)
478 GTGCAG
BsmAI 988 GAGAC GTCTC(1/5)
102 GTCTC
BsmBI 101 CGTCTC CGTCTC(1/5)
988 GAGACG
Bsp1407I 602 TGTACA T^GTACA
1316 TGTACA
BsrGI 602 TGTACA T^GTACA
1316 TGTACA
BssHII 281 GCGCGC G^CGCGC
346 GCGCGC
EcoT14I 639 CCATGG C^C[AT][AT]GG
827 CCATGG
MspA1I 759 CAGCGG C[AC]G^C[GT]G
1083 CAGCGG
NcoI 639 CCATGG C^CATGG
827 CCATGG
PpuMI 576 GGGACCC [GA]G^G[AT]CC[CT]
993 GGGTCCC
SacI 391 GAGCTC GAGCT^C
802 GAGCTC
SspBI 602 TGTACA T^GTACA
1316 TGTACA
StuI 902 AGGCCT AGG^CCT
1160 AGGCCT
StyI 639 CCATGG C^C[AT][AT]GG
827 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BanI BanII
BbeI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BsaHI BsaJI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstNI BstUI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EagI EclXI Eco52I
EcoO109I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
MaeII MnlI MslI MspI MvaI
MvnI MwoI NaeI NarI NciI
NgoMIV NlaIII NlaIV PleI PstI
RsaI Sau96I ScrFI SfcI SfoI
TaqI TseI TthHB8I