Restriction map for
pF1KB7749
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# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI AseI
AsnI AspEI AspHI AspI AvrII
BamHI BbrPI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaAI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI Eam1105I Eco81I
EcoNI EcoO65I EcoRV FbaI FseI
HindIII HpaI KspI MaeIII MfeI
MluI MroI MseI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI ScaI SexAI SfaNI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SspBI SspI StuI
SwaI Tru9I Tsp45I Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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Acc65I 907 GGTACC G^GTACC
AcsI 1298 GAATTC [GA]^AATT[CT]
AlwI 744 GGATC GGATC(4/5)
Aor51HI 980 AGCGCT AGC^GCT
ApoI 1298 GAATTC [GA]^AATT[CT]
Asp700I 621 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 907 GGTACC G^GTACC
AvaI 1176 CTCGGG C^[CT]CG[GA]G
BbsI 1093 GTCTTC GAAGAC(2/6)
BcgI 139 CGACCCGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 236 CTAG C^TAG
BglII 707 AGATCT A^GATCT
BpuAI 1093 GTCTTC GAAGAC(2/6)
BsaBI 702 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 380 CTCCTC GAGGAG(10/8)
BsiEI 652 CGGCCG CG[GA][CT]^CG
BsoBI 1176 CTCGGG C^[CT]CG[GA]G
BssSI 594 CACGAG CACGAG(-5/-1)
BstYI 707 AGATCT [GA]^GATC[CT]
EagI 652 CGGCCG C^GGCCG
EarI 366 CTCTTC CTCTTC(1/4)
EclXI 652 CGGCCG C^GGCCG
Eco47III 980 AGCGCT AGC^GCT
Eco52I 652 CGGCCG C^GGCCG
EcoRI 1298 GAATTC G^AATTC
EcoT22I 838 ATGCAT ATGCA^T
HincII 850 GTCAAC GT[CT]^[GA]AC
HindII 850 GTCAAC GT[CT]^[GA]AC
KpnI 907 GGTACC GGTAC^C
Ksp632I 366 CTCTTC CTCTTC(1/4)
MaeI 236 CTAG C^TAG
MaeII 769 ACGT A^CGT
MamI 702 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 707 AGATCT [GA]^GATC[CT]
MslI 1033 CACGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 650 GCCGGC GCC^GGC
NcoI 1184 CCATGG C^CATGG
NgoMIV 650 GCCGGC G^CCGGC
NsiI 838 ATGCAT ATGCA^T
NspI 761 GCATGC [GA]CATG^[CT]
PleI 481 GAGTC GAGTC(4/5)
PpuMI 1066 GGGACCC [GA]G^G[AT]CC[CT]
PstI 1045 CTGCAG CTGCA^G
SbfI 1044 CCTGCAGG CCTGCA^GG
SfcI 1045 CTGCAG C^T[GA][CT]AG
SphI 761 GCATGC GCATG^C
Sse8387I 1044 CCTGCAGG CCTGCA^GG
TaqI 486 TCGA T^CGA
TfiI 720 GAATC G^A[AT]TC
Tsp509I 1299 AATT ^AATT
TthHB8I 486 TCGA T^CGA
XhoII 707 AGATCT [GA]^GATC[CT]
XmnI 621 GAAAGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 1067 GGACC G^G[AT]CC
1266 GGACC
AviII 215 TGCGCA TGC^GCA
955 TGCGCA
BalI 452 TGGCCA TGG^CCA
671 TGGCCA
BbeI 118 GGCGCC GGCGC^C
1180 GGCGCC
BbvCI 313 CCTCAGC CCTCAGC(-5/-2)
325 CCTCAGC
BglI 412 GCCAACGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1043 GCCTGCAGGGC
BsgI 268 CTGCAC GTGCAG(16/14)
335 CTGCAC
BsmI 387 GAATGC GAATGC(1/-1)
840 GCATTC
BstF5I 1282 CATCC GGATG(2/0)
473 GGATG
DraII 1066 GGGACCC [GA]G^G[ACGT]CC[CT]
1236 GGGGCCT
DsaI 1184 CCATGG C^C[GA][CT]GG
1227 CCACGG
EcoO109I 1066 GGGACCC [GA]G^G[ACGT]CC[CT]
1236 GGGGCCT
EcoT14I 185 CCAAGG C^C[AT][AT]GG
1184 CCATGG
FokI 1282 CATCC GGATG(9/13)
473 GGATG
FspI 215 TGCGCA TGC^GCA
955 TGCGCA
HinfI 481 GAGTC G^A[ACGT]TC
720 GAATC
KasI 118 GGCGCC G^GCGCC
1180 GGCGCC
MluNI 452 TGGCCA TGG^CCA
671 TGGCCA
MscI 452 TGGCCA TGG^CCA
671 TGGCCA
NarI 118 GGCGCC GG^CGCC
1180 GGCGCC
PvuII 604 CAGCTG CAG^CTG
951 CAGCTG
SfoI 118 GGCGCC GGC^GCC
1180 GGCGCC
StyI 185 CCAAGG C^C[AT][AT]GG
1184 CCATGG
TspRI 449 CAGTG CA[GC]TG[ACGT][ACGT]^
690 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrFI BsrI
BssHII BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
TseI