Restriction map for
pF1KB7747
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspHI AvaI AviII BalI
BamHI BanI BanII BbeI BbrPI
BbvCI BfrI BglI BglII BmyI
BsaAI BsaBI BseAI BsiEI BsiHKAI
BsiWI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstF5I BstPI BstZ17I Bsu36I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FauI FokI FseI FspI
HincII HindII HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Van91I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 11 CGCG CG^CG
AcsI 483 GAATTC [GA]^AATT[CT]
AcyI 1155 GGCGTC G[GA]^CG[CT]C
AhdI 1111 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 483 GAATTC [GA]^AATT[CT]
Asp700I 1164 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1111 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 37 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 952 CCTAGG C^CTAGG
BbsI 43 GTCTTC GAAGAC(2/6)
BcgI 288 CGAAGTTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 348 GGATAC GTATCC(6/5)
BclI 968 TGATCA T^GATCA
BlnI 952 CCTAGG C^CTAGG
BlpI 76 GCTAAGC GC^T[ACGT]AGC
BpmI 562 CTGGAG CTGGAG(16/14)
Bpu1102I 76 GCTAAGC GC^T[ACGT]AGC
BpuAI 43 GTCTTC GAAGAC(2/6)
BsaHI 1155 GGCGTC G[GA]^CG[CT]C
BsaI 646 GGTCTC GGTCTC(1/5)
BsaWI 505 TCCGGT [AT]^CCGG[AT]
BsmBI 379 CGTCTC CGTCTC(1/5)
BsrBI 884 GAGCGG CCGCTC(-3/-3)
BsrDI 236 GCAATG GCAATG(2/0)
BsrFI 17 GCCGGC [GA]^CCGG[CT]
BssSI 776 CTCGTG CACGAG(-5/-1)
BstUI 11 CGCG CG^CG
BstXI 812 CCAACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 76 GCTAAGC GC^T[ACGT]AGC
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
DraI 943 TTTAAA TTT^AAA
DraIII 1262 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1140 TGGCCG [CT]^GGCC[GA]
Eam1105I 1111 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 483 GAATTC G^AATTC
EcoRV 394 GATATC GAT^ATC
FbaI 968 TGATCA T^GATCA
HaeII 125 AGCGCC [GA]GCGC^[CT]
Hin1I 1155 GGCGTC G[GA]^CG[CT]C
HindIII 802 AAGCTT A^AGCTT
HphI 213 GGTGA GGTGA(8/7)
MseI 944 TTAA T^TAA
MvnI 11 CGCG CG^CG
NaeI 17 GCCGGC GCC^GGC
NgoMIV 17 GCCGGC G^CCGGC
NspI 448 GCATGT [GA]CATG^[CT]
PflFI 37 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 204 GACTC GAGTC(4/5)
PpuMI 948 AGGTCCT [GA]G^G[AT]CC[CT]
StuI 1277 AGGCCT AGG^CCT
TaqI 416 TCGA T^CGA
TfiI 990 GAATC G^A[AT]TC
Tru9I 944 TTAA T^TAA
Tth111I 37 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 416 TCGA T^CGA
XmnI 1164 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 1221 ACGTGT A^C[GA][CT]GT
1235 ACGTGT
AlwNI 266 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
901 CAGGAGCTG
BseRI 312 CTCCTC GAGGAG(10/8)
421 GAGGAG
BsmFI 823 GGGAC GGGAC(10/14)
26 GTCCC
BstYI 808 AGATCC [GA]^GATC[CT]
1001 AGATCC
CfoI 12 GCGC GCG^C
126 GCGC
DraII 948 AGGTCCT [GA]G^G[ACGT]CC[CT]
1091 AGGCCCC
EarI 649 CTCTTC CTCTTC(1/4)
108 GAAGAG
EcoO109I 948 AGGTCCT [GA]G^G[ACGT]CC[CT]
1091 AGGCCCC
HgaI 378 GCGTC GACGC(5/10)
1156 GCGTC
HhaI 12 GCGC GCG^C
126 GCGC
HinP1I 12 GCGC G^CGC
126 GCGC
HinfI 204 GACTC G^A[ACGT]TC
990 GAATC
Ksp632I 649 CTCTTC CTCTTC(1/4)
108 GAAGAG
MflI 808 AGATCC [GA]^GATC[CT]
1001 AGATCC
MslI 232 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1183 CACAAAAATG
NlaIV 823 GGGACC GG[ACGT]^[ACGT]CC
1092 GGCCCC
PvuII 266 CAGCTG CAG^CTG
530 CAGCTG
Tsp509I 466 AATT ^AATT
484 AATT
XhoII 808 AGATCC [GA]^GATC[CT]
1001 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BcnI BfaI BsaJI BsgI
BsiYI BslI BsmAI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DsaI EcoRII EcoT14I Fnu4HI HaeIII
HapII HpaII ItaI MaeI MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TseI Tsp45I
TspRI