Restriction map for
pF1KB7741
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwNI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsiWI BsoBI BspEI BspLU11I
BsrBI BsrDI BsrFI BssHII BssSI
Bst1107I BstZ17I Bsu36I Cac8I CelII
Cfr10I CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT14I FbaI FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspA1I MunI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI StyI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccII 828 CGCG CG^CG
AcsI 722 AAATTC [GA]^AATT[CT]
AcyI 414 GACGCC G[GA]^CG[CT]C
Alw44I 467 GTGCAC G^TGCAC
AlwI 1287 GGATC GGATC(4/5)
ApaLI 467 GTGCAC G^TGCAC
ApoI 722 AAATTC [GA]^AATT[CT]
Asp700I 324 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 584 GGTCC G^G[AT]CC
BbvI 311 GCAGC GCAGC(8/12)
BcnI 1253 CCGGG CC^[GC]GG
BglII 820 AGATCT A^GATCT
BsaHI 414 GACGCC G[GA]^CG[CT]C
BsaI 498 GAGACC GGTCTC(1/5)
BseRI 46 GAGGAG GAGGAG(10/8)
BsgI 1131 GTGCAG GTGCAG(16/14)
BsiEI 125 CGGTCG CG[GA][CT]^CG
BsmBI 412 GAGACG CGTCTC(1/5)
BsmI 508 GAATGC GAATGC(1/-1)
Bsp1407I 1102 TGTACA T^GTACA
BspDI 700 ATCGAT AT^CGAT
BspHI 1233 TCATGA T^CATGA
BspMI 793 GCAGGT ACCTGC(4/8)
BsrGI 1102 TGTACA T^GTACA
BstAPI 512 GCAAGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 796 GGTTACC G^GT[ACGT]ACC
BstF5I 21 GGATG GGATG(2/0)
BstPI 796 GGTTACC G^GT[ACGT]ACC
BstUI 828 CGCG CG^CG
BstXI 1263 CCAGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 820 AGATCT [GA]^GATC[CT]
Cfr13I 584 GGTCC G^G[ACGT]CC
ClaI 700 ATCGAT AT^CGAT
DraII 583 AGGTCCT [GA]G^G[ACGT]CC[CT]
EaeI 1250 TGGCCG [CT]^GGCC[GA]
EcoO109I 583 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 796 GGTTACC G^GT[ACGT]ACC
EcoT22I 652 ATGCAT ATGCA^T
FauI 521 GCGGG CCCGC(4/6)
Fnu4HI 311 GCAGC GC^[ACGT]GC
FokI 21 GGATG GGATG(9/13)
HapII 1253 CCGG C^CGG
Hin1I 414 GACGCC G[GA]^CG[CT]C
HpaII 1253 CCGG C^CGG
ItaI 311 GCAGC GC^[ACGT]GC
MflI 820 AGATCT [GA]^GATC[CT]
MspI 1253 CCGG C^CGG
MvnI 828 CGCG CG^CG
NciI 1253 CCGGG CC^[GC]GG
NdeI 1094 CATATG CA^TATG
NlaIV 113 GGAACC GG[ACGT]^[ACGT]CC
NsiI 652 ATGCAT ATGCA^T
PflMI 168 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 583 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1233 TCATGA T^CATGA
Sau96I 584 GGTCC G^G[ACGT]CC
SfcI 84 CTACAG C^T[GA][CT]AG
SspBI 1102 TGTACA T^GTACA
TseI 311 GCAGC G^C[AT]GC
Van91I 168 CCAAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 820 AGATCT [GA]^GATC[CT]
XmnI 324 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
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AciI 791 CCGC CCGC(-3/-1)
521 GCGG
AfaI 165 GTAC GT^AC
1103 GTAC
BsmFI 962 GTCCC GGGAC(10/14)
969 GTCCC
BstBI 635 TTCGAA TT^CGAA
1058 TTCGAA
CfoI 474 GCGC GCG^C
829 GCGC
HgaI 96 GACGC GACGC(5/10)
414 GACGC
HhaI 474 GCGC GCG^C
829 GCGC
HinP1I 474 GCGC G^CGC
829 GCGC
MaeII 216 ACGT A^CGT
967 ACGT
MseI 182 TTAA T^TAA
533 TTAA
MwoI 305 GCAAATGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
512 GCAAGGTGTGC
NspV 635 TTCGAA TT^CGAA
1058 TTCGAA
RsaI 165 GTAC GT^AC
1103 GTAC
SacI 365 GAGCTC GAGCT^C
989 GAGCTC
SfuI 635 TTCGAA TT^CGAA
1058 TTCGAA
Tru9I 182 TTAA T^TAA
533 TTAA
Tsp45I 94 GTGAC ^GT[GC]AC
266 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AluI AspHI BanII BbsI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI DdeI DpnII EcoRII HaeIII
HinfI HphI MaeIII MboI MboII
MnlI MslI MvaI NdeII NlaIII
PleI Sau3AI ScrFI TaqI TfiI
Tsp509I TspRI TthHB8I