Restriction map for
pF1KB7731
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AlwI
AocI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BciVI BclI BfrI
BlnI BlpI Bpu1102I BsaI BsaWI
BseAI BsiWI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI Eam1105I
EarI Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MaeIII MfeI MluI MroI
MseI MunI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SspBI SspI StuI SwaI
Tru9I Tsp45I Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AcsI 634 GAATTC [GA]^AATT[CT]
Alw44I 42 GTGCAC G^TGCAC
Aor51HI 980 AGCGCT AGC^GCT
ApaI 436 GGGCCC GGGCC^C
ApaLI 42 GTGCAC G^TGCAC
ApoI 634 GAATTC [GA]^AATT[CT]
BbrPI 835 CACGTG CAC^GTG
BbsI 1117 GTCTTC GAAGAC(2/6)
BbvCI 455 CCTCAGC CCTCAGC(-5/-2)
BcgI 1079 GCAACTTCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1117 GTCTTC GAAGAC(2/6)
BsaBI 711 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 775 CTGCAC GTGCAG(16/14)
BsmBI 1253 CGTCTC CGTCTC(1/5)
BsrBI 814 CCGCTC CCGCTC(-3/-3)
BssHII 608 GCGCGC G^CGCGC
BssSI 603 CACGAG CACGAG(-5/-1)
DraII 23 AGGCCCT [GA]G^G[ACGT]CC[CT]
Eco47III 980 AGCGCT AGC^GCT
EcoO109I 23 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 634 GAATTC G^AATTC
HphI 388 TCACC GGTGA(8/7)
MamI 711 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 1195 CACCACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NheI 787 GCTAGC G^CTAGC
NspI 269 GCATGC [GA]CATG^[CT]
PflMI 1008 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 835 CACGTG CAC^GTG
PmlI 835 CACGTG CAC^GTG
SacI 213 GAGCTC GAGCT^C
SbfI 1050 CCTGCAGG CCTGCA^GG
SphI 269 GCATGC GCATG^C
Sse8387I 1050 CCTGCAGG CCTGCA^GG
Van91I 1008 CCATCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 294 CCAGGGTGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspHI 42 GTGCAC G[AT]GC[AT]^C
213 GAGCTC
AvaII 753 GGACC G^G[AT]CC
1204 GGACC
BalI 680 TGGCCA TGG^CCA
831 TGGCCA
BfaI 449 CTAG C^TAG
788 CTAG
BglI 563 GCCCGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
940 GCCTACACGGC
BpmI 28 CTGGAG CTGGAG(16/14)
401 CTGGAG
BsaAI 835 CACGTG [CT]AC^GT[GA]
1069 TACGTG
BseRI 359 CTCCTC GAGGAG(10/8)
453 CTCCTC
BsiEI 274 CGGCCG CG[GA][CT]^CG
661 CGGCCG
BsiHKAI 42 GTGCAC G[AT]GC[AT]^C
213 GAGCTC
BsmAI 498 GTCTC GTCTC(1/5)
1254 GTCTC
BsmFI 1260 GGGAC GGGAC(10/14)
795 GTCCC
BspMI 686 ACCTGC ACCTGC(4/8)
1049 ACCTGC
EagI 274 CGGCCG C^GGCCG
661 CGGCCG
EclXI 274 CGGCCG C^GGCCG
661 CGGCCG
Eco52I 274 CGGCCG C^GGCCG
661 CGGCCG
MaeI 449 CTAG C^TAG
788 CTAG
MaeII 836 ACGT A^CGT
1070 ACGT
MluNI 680 TGGCCA TGG^CCA
831 TGGCCA
MscI 680 TGGCCA TGG^CCA
831 TGGCCA
NaeI 659 GCCGGC GCC^GGC
783 GCCGGC
NgoMIV 659 GCCGGC G^CCGGC
783 GCCGGC
PleI 496 GAGTC GAGTC(4/5)
1090 GAGTC
PstI 536 CTGCAG CTGCA^G
1051 CTGCAG
TfiI 693 GAATC G^A[AT]TC
1182 GATTC
Tsp509I 623 AATT ^AATT
635 AATT
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# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI BanI BanII BbeI BbvI
BcnI BglII BmyI BsaHI BsaJI
BsiYI BslI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
MboI MboII MflI MnlI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StyI TaqI
TseI TspRI TthHB8I XhoII