Restriction map for
pF1KB7726
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AflIII AgeI AhdI AocI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BanI
BbeI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaI BseAI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI DsaI Eam1105I
EarI Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
HindIII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 115 GACGTC GACGT^C
AcsI 898 GAATTT [GA]^AATT[CT]
AcyI 115 GACGTC G[GA]^CG[CT]C
AlwNI 314 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 27 AGCGCT AGC^GCT
ApaI 1435 GGGCCC GGGCC^C
ApoI 898 GAATTT [GA]^AATT[CT]
BamHI 104 GGATCC G^GATCC
BfaI 334 CTAG C^TAG
BsaHI 115 GACGTC G[GA]^CG[CT]C
BsaWI 1306 ACCGGA [AT]^CCGG[AT]
BsiEI 338 CGGCCG CG[GA][CT]^CG
BstYI 104 GGATCC [GA]^GATC[CT]
EagI 338 CGGCCG C^GGCCG
EclXI 338 CGGCCG C^GGCCG
Eco47III 27 AGCGCT AGC^GCT
Eco52I 338 CGGCCG C^GGCCG
EcoNI 466 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 115 GACGTC G[GA]^CG[CT]C
MaeI 334 CTAG C^TAG
MflI 104 GGATCC [GA]^GATC[CT]
MseI 1097 TTAA T^TAA
MslI 1407 CACCAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI 337 GCGGCCGC GC^GGCCGC
NspI 376 GCATGC [GA]CATG^[CT]
SexAI 471 ACCAGGT A^CC[AT]GGT
SmaI 241 CCCGGG CCC^GGG
SphI 376 GCATGC GCATG^C
TaqI 522 TCGA T^CGA
TfiI 517 GAATC G^A[AT]TC
Tru9I 1097 TTAA T^TAA
TthHB8I 522 TCGA T^CGA
XhoII 104 GGATCC [GA]^GATC[CT]
XmaCI 241 CCCGGG C^CCGGG
XmaI 241 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
Alw44I 1048 GTGCAC G^TGCAC
1414 GTGCAC
ApaLI 1048 GTGCAC G^TGCAC
1414 GTGCAC
AvaI 241 CCCGGG C^[CT]CG[GA]G
1429 CTCGGG
BbrPI 364 CACGTG CAC^GTG
1207 CACGTG
BbsI 973 GAAGAC GAAGAC(2/6)
1005 GTCTTC
BpuAI 973 GAAGAC GAAGAC(2/6)
1005 GTCTTC
BsaAI 364 CACGTG [CT]AC^GT[GA]
1207 CACGTG
BseRI 60 CTCCTC GAGGAG(10/8)
829 CTCCTC
BsgI 320 CTGCAC GTGCAG(16/14)
790 GTGCAG
BsmAI 689 GAGAC GTCTC(1/5)
1162 GAGAC
BsmBI 689 GAGACG CGTCTC(1/5)
1162 GAGACG
BsoBI 241 CCCGGG C^[CT]CG[GA]G
1429 CTCGGG
Bsp1407I 287 TGTACA T^GTACA
1182 TGTACA
BspMI 1249 ACCTGC ACCTGC(4/8)
1271 ACCTGC
BsrFI 1001 GCCGGT [GA]^CCGG[CT]
1100 ACCGGC
BsrGI 287 TGTACA T^GTACA
1182 TGTACA
Cfr10I 1001 GCCGGT [GA]^CCGG[CT]
1100 ACCGGC
DdeI 145 CTCAG C^T[ACGT]AG
611 CTCAG
EaeI 338 CGGCCG [CT]^GGCC[GA]
1102 CGGCCA
EcoT14I 566 CCAAGG C^C[AT][AT]GG
956 CCTTGG
HaeII 27 AGCGCT [GA]GCGC^[CT]
485 GGCGCT
HincII 656 GTCAAC GT[CT]^[GA]AC
1361 GTCAAC
HindII 656 GTCAAC GT[CT]^[GA]AC
1361 GTCAAC
HphI 862 TCACC GGTGA(8/7)
1038 TCACC
MaeIII 118 GTCAC ^GT[ACGT]AC
1037 GTCAC
MspA1I 906 CAGCTG C[AC]G^C[GT]G
994 CAGCTG
NlaIII 285 CATG CATG^
377 CATG
PmaCI 364 CACGTG CAC^GTG
1207 CACGTG
PmlI 364 CACGTG CAC^GTG
1207 CACGTG
PpuMI 246 GGGTCCC [GA]G^G[AT]CC[CT]
741 AGGACCC
PvuII 906 CAGCTG CAG^CTG
994 CAGCTG
SmlI 845 CTCAAG C^T[CT][GA]AG
1282 CTCAAG
SspBI 287 TGTACA T^GTACA
1182 TGTACA
StyI 566 CCAAGG C^C[AT][AT]GG
956 CCTTGG
Tsp45I 118 GTCAC ^GT[GC]AC
1037 GTCAC
Tsp509I 195 AATT ^AATT
899 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII DraII EcoO109I EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeII MboI MboII MnlI MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
TseI TspRI