Restriction map for
pF1KB7715
##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaI BsiHKAI BsiWI BsmAI
BsmBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CelII ClaI DraIII Eam1105I Eco47III
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FseI FspI HaeII HincII HindII
HindIII HpaI KasI KpnI KspI
MaeII MfeI MluI MluNI MscI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TfiI
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AcyI 766 GACGCC G[GA]^CG[CT]C
AfaI 573 GTAC GT^AC
AgeI 290 ACCGGT A^CCGGT
AlwNI 34 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 340 CCTGAGG CC^T[ACGT]AGG
ApaI 727 GGGCCC GGGCC^C
AspI 1198 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 110 GGATCC G^GATCC
BciVI 66 GTATCC GTATCC(6/5)
BcnI 709 CCCGG CC^[GC]GG
BfaI 364 CTAG C^TAG
BsaHI 766 GACGCC G[GA]^CG[CT]C
BsmI 601 GCATTC GAATGC(1/-1)
BssSI 1096 CTCGTG CACGAG(-5/-1)
BstYI 110 GGATCC [GA]^GATC[CT]
Bsu36I 340 CCTGAGG CC^T[ACGT]AGG
CpoI 287 CGGACCG CG^G[AT]CCG
DrdI 285 GACGGACCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 908 CCGTGG C^C[GA][CT]GG
EaeI 761 CGGCCG [CT]^GGCC[GA]
EagI 761 CGGCCG C^GGCCG
EclXI 761 CGGCCG C^GGCCG
Eco52I 761 CGGCCG C^GGCCG
Eco81I 340 CCTGAGG CC^T[ACGT]AGG
EcoT14I 115 CCTTGG C^C[AT][AT]GG
Hin1I 766 GACGCC G[GA]^CG[CT]C
HinfI 510 GAGTC G^A[ACGT]TC
MaeI 364 CTAG C^TAG
MflI 110 GGATCC [GA]^GATC[CT]
NciI 709 CCCGG CC^[GC]GG
PflFI 1198 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 290 ACCGGT A^CCGGT
PleI 510 GAGTC GAGTC(4/5)
PvuII 1111 CAGCTG CAG^CTG
RsaI 573 GTAC GT^AC
RsrII 287 CGGACCG CG^G[AT]CCG
SexAI 1199 ACCAGGT A^CC[AT]GGT
StyI 115 CCTTGG C^C[AT][AT]GG
Tsp509I 333 AATT ^AATT
TspRI 34 CAGTG CA[GC]TG[ACGT][ACGT]^
Tth111I 1198 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 70 CCAGGACTGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 110 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 111 GATCC GGATC(4/5)
110 GGATC
Asp700I 804 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1054 GAAGAGTTTC
BanI 712 GGCACC G^G[CT][GA]CC
815 GGCACC
BglI 668 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
848 GCCGGCAGGGC
BsaBI 139 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
612 GATGGTGATC
BseRI 91 GAGGAG GAGGAG(10/8)
1183 GAGGAG
BsgI 838 CTGCAC GTGCAG(16/14)
1099 GTGCAG
BsiEI 414 CGGTCG CG[GA][CT]^CG
761 CGGCCG
BspMI 664 ACCTGC ACCTGC(4/8)
792 GCAGGT
DdeI 341 CTGAG C^T[ACGT]AG
691 CTGAG
DraI 187 TTTAAA TTT^AAA
199 TTTAAA
DraII 734 AGGGCCT [GA]G^G[ACGT]CC[CT]
1175 AGGCCCC
EcoO109I 734 AGGGCCT [GA]G^G[ACGT]CC[CT]
1175 AGGCCCC
MamI 139 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
612 GATGGTGATC
NspI 62 GCATGT [GA]CATG^[CT]
101 GCATGT
PstI 894 CTGCAG CTGCA^G
991 CTGCAG
SfaNI 157 GATGC GCATC(5/9)
1170 GATGC
SfcI 894 CTGCAG C^T[GA][CT]AG
991 CTGCAG
StuI 173 AGGCCT AGG^CCT
500 AGGCCT
XmnI 804 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1054 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AccIII AciI AluI AvaI
AvaII BanII BbsI BbvI BclI
BmyI BpmI BpuAI BsaJI BsaWI
BseAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspEI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DpnII EarI
EcoRII FauI FbaI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NdeII NgoMIV NlaIII
NlaIV SapI Sau3AI Sau96I ScrFI
TaqI Tru9I TseI Tsp45I TthHB8I