Restriction map for
pF1KB7705
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AatII AccII AccIII AclI AcsI
AcyI AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BcgI BciVI BglI
BglII BlnI BlpI Bpu1102I BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmI BsoBI Bsp1407I
BspEI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII Hin1I HincII HindII HpaI
KasI KspI MfeI MflI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 489 GGTACC G^GTACC
AccI 211 GTAGAC GT^[AC][GT]AC
AciI 435 CCGC CCGC(-3/-1)
AlwI 91 GGATC GGATC(4/5)
AlwNI 1081 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 489 GGTACC G^GTACC
BanI 489 GGTACC G^G[CT][GA]CC
BanII 918 GAGCTC G[GA]GC[CT]^C
BbrPI 73 CACGTG CAC^GTG
BbsI 510 GTCTTC GAAGAC(2/6)
BbvCI 1078 CCTCAGC CCTCAGC(-5/-2)
BcnI 284 CCCGG CC^[GC]GG
BpmI 398 CTGGAG CTGGAG(16/14)
BpuAI 510 GTCTTC GAAGAC(2/6)
BsaAI 73 CACGTG [CT]AC^GT[GA]
BsaBI 142 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 921 CTCCTC GAGGAG(10/8)
BsgI 1087 CTGCAC GTGCAG(16/14)
BspDI 329 ATCGAT AT^CGAT
BspHI 857 TCATGA T^CATGA
BspMI 982 GCAGGT ACCTGC(4/8)
BsrFI 1065 GCCGGT [GA]^CCGG[CT]
BssSI 1011 CACGAG CACGAG(-5/-1)
BstNI 1071 CCAGG CC^[AT]GG
CfoI 474 GCGC GCG^C
Cfr10I 1065 GCCGGT [GA]^CCGG[CT]
ClaI 329 ATCGAT AT^CGAT
DsaI 608 CCATGG C^C[GA][CT]GG
EaeI 946 CGGCCA [CT]^GGCC[GA]
EcoRII 1071 CCAGG ^CC[AT]GG
HgaI 366 GCGTC GACGC(5/10)
HhaI 474 GCGC GCG^C
HinP1I 474 GCGC G^CGC
HindIII 241 AAGCTT A^AGCTT
KpnI 489 GGTACC GGTAC^C
MaeII 74 ACGT A^CGT
MaeIII 452 GTAAC ^GT[ACGT]AC
MamI 142 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 435 CCGCTG C[AC]G^C[GT]G
MvaI 1071 CCAGG CC^[AT]GG
NciI 284 CCCGG CC^[GC]GG
NcoI 608 CCATGG C^CATGG
PflMI 703 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 73 CACGTG CAC^GTG
PmlI 73 CACGTG CAC^GTG
PstI 438 CTGCAG CTGCA^G
RcaI 857 TCATGA T^CATGA
SacI 918 GAGCTC GAGCT^C
Van91I 703 CCAACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BclI 147 TGATCA T^GATCA
162 TGATCA
BfaI 506 CTAG C^TAG
772 CTAG
BssKI 284 CCCGG ^CC[ACGT]GG
1071 CCAGG
EarI 122 CTCTTC CTCTTC(1/4)
578 GAAGAG
FbaI 147 TGATCA T^GATCA
162 TGATCA
HaeIII 712 GGCC GG^CC
947 GGCC
HapII 285 CCGG C^CGG
1066 CCGG
HpaII 285 CCGG C^CGG
1066 CCGG
Ksp632I 122 CTCTTC CTCTTC(1/4)
578 GAAGAG
MaeI 506 CTAG C^TAG
772 CTAG
MspI 285 CCGG C^CGG
1066 CCGG
NdeI 382 CATATG CA^TATG
1144 CATATG
SapI 578 GAAGAGC GCTCTTC(1/4)
121 GCTCTTC
ScrFI 284 CCCGG CC^[ACGT]GG
1071 CCAGG
Tsp509I 422 AATT ^AATT
495 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AfaI AflII AluI AspHI AvaII
BbvI BfrI BmyI BsaJI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BstF5I Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoT14I Fnu4HI FokI
HinfI HphI ItaI MboI MboII
MnlI MseI MslI MwoI NdeII
NlaIII NlaIV PleI PpuMI RsaI
Sau3AI Sau96I SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
TspRI TthHB8I