Restriction map for
pF1KB7702
##################################
# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AlwI AocI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AviII AvrII BamHI
BbeI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaHI BsaI BseAI BseRI
BsiEI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BssSI Bst1107I BstBI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MamI MfeI MflI MluI MroI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspI StuI SwaI
Tru9I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 250 CGCG CG^CG
AcsI 1243 AAATTC [GA]^AATT[CT]
AhdI 52 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 144 GTGCAC G^TGCAC
Aor51HI 188 AGCGCT AGC^GCT
ApaLI 144 GTGCAC G^TGCAC
ApoI 1243 AAATTC [GA]^AATT[CT]
AspEI 52 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1007 TGGCCA TGG^CCA
BbsI 853 GTCTTC GAAGAC(2/6)
BcgI 374 CGATGGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1009 GCCACCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 853 GTCTTC GAAGAC(2/6)
BsaWI 405 ACCGGA [AT]^CCGG[AT]
BsmAI 361 GAGAC GTCTC(1/5)
Bsp1407I 293 TGTACA T^GTACA
BsrGI 293 TGTACA T^GTACA
BssHII 249 GCGCGC G^CGCGC
BstEII 975 GGTCACC G^GT[ACGT]ACC
BstPI 975 GGTCACC G^GT[ACGT]ACC
BstUI 250 CGCG CG^CG
BstXI 446 CCAAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DpnII 168 GATC ^GATC
DraIII 1212 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 52 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1126 CTCTTC CTCTTC(1/4)
Eco47III 188 AGCGCT AGC^GCT
EcoNI 1266 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 975 GGTCACC G^GT[ACGT]ACC
HaeII 188 AGCGCT [GA]GCGC^[CT]
HinfI 336 GATTC G^A[ACGT]TC
Ksp632I 1126 CTCTTC CTCTTC(1/4)
MaeIII 976 GTCAC ^GT[ACGT]AC
MboI 168 GATC ^GATC
MluNI 1007 TGGCCA TGG^CCA
MscI 1007 TGGCCA TGG^CCA
MvnI 250 CGCG CG^CG
NaeI 774 GCCGGC GCC^GGC
NcoI 509 CCATGG C^CATGG
NdeII 168 GATC ^GATC
NgoMIV 774 GCCGGC G^CCGGC
NspI 1289 GCATGC [GA]CATG^[CT]
PpuMI 20 AGGACCT [GA]G^G[AT]CC[CT]
SacI 673 GAGCTC GAGCT^C
Sau3AI 168 GATC ^GATC
SbfI 741 CCTGCAGG CCTGCA^GG
SexAI 713 ACCAGGT A^CC[AT]GGT
SmlI 1111 CTCAAG C^T[CT][GA]AG
SphI 1289 GCATGC GCATG^C
Sse8387I 741 CCTGCAGG CCTGCA^GG
SspBI 293 TGTACA T^GTACA
TfiI 336 GATTC G^A[AT]TC
Tsp45I 976 GTCAC ^GT[GC]AC
Tsp509I 1244 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AflIII 85 ACGTGT A^C[GA][CT]GT
140 ACACGT
AspHI 144 GTGCAC G[AT]GC[AT]^C
673 GAGCTC
BanI 381 GGTGCC G^G[CT][GA]CC
523 GGTGCC
BbrPI 84 CACGTG CAC^GTG
141 CACGTG
BsiHKAI 144 GTGCAC G[AT]GC[AT]^C
673 GAGCTC
BsiWI 11 CGTACG C^GTACG
629 CGTACG
BstAPI 684 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1251 GCAGCTGCTGC
DsaI 509 CCATGG C^C[GA][CT]GG
528 CCACGG
HphI 1167 GGTGA GGTGA(8/7)
977 TCACC
PmaCI 84 CACGTG CAC^GTG
141 CACGTG
PmlI 84 CACGTG CAC^GTG
141 CACGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwNI ApaI
AvaII BanII BbvI BcnI BmyI
BpmI BsaAI BsaJI BsgI BsiYI
BslI BsmFI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MaeII
MboII MnlI MslI MspA1I MspI
MvaI MwoI NciI NlaIII NlaIV
PflMI PstI PvuII RsaI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TseI TspRI TthHB8I Van91I