Restriction map for
pF1KB7658
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# NO CUT ENZYMES ( 156 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AvaI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MslI MspA1I MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tsp45I XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccI 700 GTAGAC GT^[AC][GT]AC
AciI 64 CCGC CCGC(-3/-1)
AocI 334 CCTGAGG CC^T[ACGT]AGG
ApaI 112 GGGCCC GGGCC^C
AspI 306 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 399 GGATCC G^GATCC
BbsI 537 GTCTTC GAAGAC(2/6)
BcnI 1136 CCCGG CC^[GC]GG
BpuAI 537 GTCTTC GAAGAC(2/6)
BsmFI 260 GTCCC GGGAC(10/14)
Bsu36I 334 CCTGAGG CC^T[ACGT]AGG
DraII 811 AGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 761 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 436 GACAGCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 334 CCTGAGG CC^T[ACGT]AGG
EcoO109I 811 AGGACCC [GA]G^G[ACGT]CC[CT]
HapII 1137 CCGG C^CGG
HpaII 1137 CCGG C^CGG
MaeII 418 ACGT A^CGT
MspI 1137 CCGG C^CGG
NciI 1136 CCCGG CC^[GC]GG
NdeI 1085 CATATG CA^TATG
PflFI 306 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 811 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1060 CTGCAG CTGCA^G
SacI 228 GAGCTC GAGCT^C
SmlI 231 CTCAAG C^T[CT][GA]AG
Tth111I 306 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 678 TCTAGA T^CTAGA
XcmI 164 CCAAGCCAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 896 GAATTC [GA]^AATT[CT]
954 GAATTC
AfaI 750 GTAC GT^AC
1133 GTAC
ApoI 896 GAATTC [GA]^AATT[CT]
954 GAATTC
Asp700I 855 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1023 GAAAACCTTC
AspHI 142 GAGCAC G[AT]GC[AT]^C
228 GAGCTC
AvaII 177 GGTCC G^G[AT]CC
812 GGACC
BanII 112 GGGCCC G[GA]GC[CT]^C
228 GAGCTC
BbvI 579 GCAGC GCAGC(8/12)
7 GCTGC
BfaI 133 CTAG C^TAG
679 CTAG
BpmI 139 CTGGAG CTGGAG(16/14)
821 CTGGAG
BsiHKAI 142 GAGCAC G[AT]GC[AT]^C
228 GAGCTC
BspHI 972 TCATGA T^CATGA
1068 TCATGA
BstYI 399 GGATCC [GA]^GATC[CT]
492 AGATCC
DpnII 400 GATC ^GATC
493 GATC
EcoRI 896 GAATTC G^AATTC
954 GAATTC
Fnu4HI 7 GCTGC GC^[ACGT]GC
579 GCAGC
HaeIII 82 GGCC GG^CC
113 GGCC
HindIII 271 AAGCTT A^AGCTT
858 AAGCTT
ItaI 7 GCTGC GC^[ACGT]GC
579 GCAGC
MaeI 133 CTAG C^TAG
679 CTAG
MaeIII 459 GTTAC ^GT[ACGT]AC
655 GTTAC
MboI 400 GATC ^GATC
493 GATC
MflI 399 GGATCC [GA]^GATC[CT]
492 AGATCC
MseI 779 TTAA T^TAA
1004 TTAA
MwoI 27 GCCCCCAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1053 GCTTCATCTGC
NdeII 400 GATC ^GATC
493 GATC
PflMI 760 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1012 CCAGAATGTGG
RcaI 972 TCATGA T^CATGA
1068 TCATGA
RsaI 750 GTAC GT^AC
1133 GTAC
Sau3AI 400 GATC ^GATC
493 GATC
SfaNI 100 GATGC GCATC(5/9)
252 GCATC
SfcI 509 CTGTAG C^T[GA][CT]AG
1060 CTGCAG
TaqI 96 TCGA T^CGA
726 TCGA
TfiI 559 GATTC G^A[AT]TC
980 GAATC
Tru9I 779 TTAA T^TAA
1004 TTAA
TseI 7 GCTGC G^C[AT]GC
579 GCAGC
Tsp509I 897 AATT ^AATT
955 AATT
TthHB8I 96 TCGA T^CGA
726 TCGA
Van91I 760 CCACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1012 CCAGAATGTGG
XhoII 399 GGATCC [GA]^GATC[CT]
492 AGATCC
XmnI 855 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1023 GAAAACCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI AlwI BmyI BsaI BsaJI
BseRI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cfr13I
DdeI EcoRII EcoT14I FokI HinfI
HphI MboII MnlI MvaI NlaIII
NlaIV PleI Sau96I ScrFI StyI
TspRI