Restriction map for
pF1KB7649
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# NO CUT ENZYMES ( 146 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BalI BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BlnI BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DraI DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI StuI StyI
SwaI Tth111I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AcsI 312 GAATTT [GA]^AATT[CT]
AfaI 429 GTAC GT^AC
Alw44I 855 GTGCAC G^TGCAC
ApaI 27 GGGCCC GGGCC^C
ApaLI 855 GTGCAC G^TGCAC
ApoI 312 GAATTT [GA]^AATT[CT]
AspHI 855 GTGCAC G[AT]GC[AT]^C
BamHI 687 GGATCC G^GATCC
BanI 868 GGCACC G^G[CT][GA]CC
BcgI 62 GCAAGGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 50 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 247 AGATCT A^GATCT
BlpI 544 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 544 GCTCAGC GC^T[ACGT]AGC
BsaI 266 GGTCTC GGTCTC(1/5)
BseRI 269 CTCCTC GAGGAG(10/8)
BsiHKAI 855 GTGCAC G[AT]GC[AT]^C
BsmAI 267 GTCTC GTCTC(1/5)
BsmFI 824 GGGAC GGGAC(10/14)
BssSI 52 CTCGTG CACGAG(-5/-1)
BstF5I 298 CATCC GGATG(2/0)
BstXI 506 CCAGCGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 544 GCTCAGC GC^T[ACGT]AGC
CfoI 353 GCGC GCG^C
DsaI 37 CCGTGG C^C[GA][CT]GG
EarI 453 CTCTTC CTCTTC(1/4)
FokI 298 CATCC GGATG(9/13)
HaeII 352 AGCGCC [GA]GCGC^[CT]
HhaI 353 GCGC GCG^C
HinP1I 353 GCGC G^CGC
Ksp632I 453 CTCTTC CTCTTC(1/4)
MaeII 640 ACGT A^CGT
MseI 719 TTAA T^TAA
MslI 732 CACTAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 927 GCATGC [GA]CATG^[CT]
PpuMI 335 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 440 CAGCTG CAG^CTG
RsaI 429 GTAC GT^AC
SfaNI 594 GATGC GCATC(5/9)
SfcI 239 CTGTAG C^T[GA][CT]AG
SphI 927 GCATGC GCATG^C
Tru9I 719 TTAA T^TAA
XcmI 638 CCACGTCTTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 701 CCTCAGG CC^T[ACGT]AGG
952 CCTGAGG
AvaII 336 GGTCC G^G[AT]CC
825 GGACC
BanII 27 GGGCCC G[GA]GC[CT]^C
622 GAGCCC
BfaI 121 CTAG C^TAG
173 CTAG
BsgI 216 CTGCAC GTGCAG(16/14)
340 CTGCAC
BsrI 793 ACTGG ACTGG(1/-1)
426 CCAGT
BstYI 247 AGATCT [GA]^GATC[CT]
687 GGATCC
Bsu36I 701 CCTCAGG CC^T[ACGT]AGG
952 CCTGAGG
DraII 26 AGGGCCC [GA]G^G[ACGT]CC[CT]
335 AGGTCCT
Eco81I 701 CCTCAGG CC^T[ACGT]AGG
952 CCTGAGG
EcoO109I 26 AGGGCCC [GA]G^G[ACGT]CC[CT]
335 AGGTCCT
FauI 509 GCGGG CCCGC(4/6)
822 GCGGG
HgaI 294 GCGTC GACGC(5/10)
745 GCGTC
HphI 276 GGTGA GGTGA(8/7)
831 TCACC
MaeI 121 CTAG C^TAG
173 CTAG
MboII 676 GAAGA GAAGA(8/7)
454 TCTTC
MflI 247 AGATCT [GA]^GATC[CT]
687 GGATCC
MspA1I 440 CAGCTG C[AC]G^C[GT]G
507 CAGCGG
Tsp509I 127 AATT ^AATT
313 AATT
XhoII 247 AGATCT [GA]^GATC[CT]
687 GGATCC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AvaI BbvI
BcnI BmyI BpmI BsaJI BsiYI
BslI BsoBI Bsp1286I BssKI BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HinfI HpaII
ItaI MaeIII MboI MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SmaI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XmaCI XmaI