Restriction map for
pF1KB7647
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspHI AviII
AvrII BamHI BbeI BbrPI BbvCI
BciVI BclI BfrI BglII BlnI
BsaBI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HhaI HinP1I HindIII HpaI
HphI KasI KspI MaeIII MamI
MfeI MflI MluI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tru9I
Tsp45I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 389 GGTACC G^GTACC
AcyI 161 GGCGTC G[GA]^CG[CT]C
AfaI 390 GTAC GT^AC
Asp718I 389 GGTACC G^GTACC
AspI 427 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 326 CCCGAG C^[CT]CG[GA]G
BalI 86 TGGCCA TGG^CCA
BanI 389 GGTACC G^G[CT][GA]CC
BbsI 831 GTCTTC GAAGAC(2/6)
BfaI 751 CTAG C^TAG
BglI 62 GCCAAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 75 GCTGAGC GC^T[ACGT]AGC
BpmI 244 CTGGAG CTGGAG(16/14)
Bpu1102I 75 GCTGAGC GC^T[ACGT]AGC
BpuAI 831 GTCTTC GAAGAC(2/6)
BsaAI 874 TACGTG [CT]AC^GT[GA]
BsaHI 161 GGCGTC G[GA]^CG[CT]C
BseRI 621 CTCCTC GAGGAG(10/8)
BsgI 422 GTGCAG GTGCAG(16/14)
BsoBI 326 CCCGAG C^[CT]CG[GA]G
CelII 75 GCTGAGC GC^T[ACGT]AGC
EaeI 86 TGGCCA [CT]^GGCC[GA]
EcoNI 372 CCTCACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 11 CCAAGG C^C[AT][AT]GG
Hin1I 161 GGCGTC G[GA]^CG[CT]C
HincII 340 GTTGAC GT[CT]^[GA]AC
HindII 340 GTTGAC GT[CT]^[GA]AC
KpnI 389 GGTACC GGTAC^C
MaeI 751 CTAG C^TAG
MluNI 86 TGGCCA TGG^CCA
MscI 86 TGGCCA TGG^CCA
MslI 51 CATCGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PflFI 427 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 37 GAGTC GAGTC(4/5)
PpuMI 398 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 826 CTGCAG CTGCA^G
RsaI 390 GTAC GT^AC
SfiI 61 GGCCAAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StyI 11 CCAAGG C^C[AT][AT]GG
Tsp509I 194 AATT ^AATT
Tth111I 427 GACCCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 347 TCCGGA T^CCGGA
434 TCCGGA
AhdI 31 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
200 GACGCTTGGTC
AlwI 146 GGATC GGATC(4/5)
276 GGATC
ApaI 409 GGGCCC GGGCC^C
740 GGGCCC
AspEI 31 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
200 GACGCTTGGTC
BsaI 167 GAGACC GGTCTC(1/5)
954 GGTCTC
BsaWI 347 TCCGGA [AT]^CCGG[AT]
434 TCCGGA
BseAI 347 TCCGGA T^CCGGA
434 TCCGGA
BsmFI 307 GGGAC GGGAC(10/14)
208 GTCCC
BspEI 347 TCCGGA T^CCGGA
434 TCCGGA
BsrBI 656 CCGCTC CCGCTC(-3/-3)
21 GAGCGG
BstXI 50 CCATCGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
294 CCATGCCACTGG
DpnII 147 GATC ^GATC
277 GATC
Eam1105I 31 GACGGGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
200 GACGCTTGGTC
FauI 743 CCCGC CCCGC(4/6)
928 GCGGG
HgaI 200 GACGC GACGC(5/10)
162 GCGTC
MaeII 56 ACGT A^CGT
875 ACGT
MboI 147 GATC ^GATC
277 GATC
MroI 347 TCCGGA T^CCGGA
434 TCCGGA
NdeII 147 GATC ^GATC
277 GATC
NlaIII 295 CATG CATG^
936 CATG
PvuII 73 CAGCTG CAG^CTG
914 CAGCTG
SapI 110 GCTCTTC GCTCTTC(1/4)
233 GCTCTTC
Sau3AI 147 GATC ^GATC
277 GATC
SfcI 826 CTGCAG C^T[GA][CT]AG
857 CTACAG
TfiI 314 GATTC G^A[AT]TC
438 GATTC
XcmI 294 CCATGCCACTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
497 CCACTGGCCCTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BcgI BcnI BmyI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI Ksp632I MboII MnlI MspA1I
MspI MvaI MwoI NciI NlaIV
Sau96I ScrFI SfaNI TaqI TseI
TspRI TthHB8I