Restriction map for
pF1KB7631
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AfaI AflII AgeI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspI AvrII BalI BamHI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BsaBI BsaI
BseAI BseRI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrFI BsrGI BssSI
Bst1107I BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluNI MroI MscI MseI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacI SalI ScaI SfiI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tru9I Tsp509I Tth111I
Van91I XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccI 31 GTAGAC GT^[AC][GT]AC
AflIII 593 ACGCGT A^C[GA][CT]GT
AhdI 748 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AluI 5 AGCT AG^CT
Alw44I 205 GTGCAC G^TGCAC
AlwI 444 GATCC GGATC(4/5)
AlwNI 481 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 398 AGCGCT AGC^GCT
ApaI 543 GGGCCC GGGCC^C
ApaLI 205 GTGCAC G^TGCAC
AspEI 748 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 449 TGCGCA TGC^GCA
BbsI 130 GAAGAC GAAGAC(2/6)
BcgI 783 CGAGAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 745 CTAG C^TAG
BpuAI 130 GAAGAC GAAGAC(2/6)
BsaWI 900 ACCGGA [AT]^CCGG[AT]
BsgI 402 CTGCAC GTGCAG(16/14)
BsiEI 324 CGACCG CG[GA][CT]^CG
BsmAI 388 GAGAC GTCTC(1/5)
BspMI 116 ACCTGC ACCTGC(4/8)
BsrI 894 CCAGT ACTGG(1/-1)
BstBI 127 TTCGAA TT^CGAA
BstF5I 459 CATCC GGATG(2/0)
BstXI 758 CCAGCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 443 AGATCC [GA]^GATC[CT]
DpnII 444 GATC ^GATC
DraII 473 AGGGCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 748 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 398 AGCGCT AGC^GCT
EcoO109I 473 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 434 CCAAGG C^C[AT][AT]GG
FokI 459 CATCC GGATG(9/13)
FspI 449 TGCGCA TGC^GCA
HphI 898 TCACC GGTGA(8/7)
MaeI 745 CTAG C^TAG
MaeIII 897 GTCAC ^GT[ACGT]AC
MboI 444 GATC ^GATC
MflI 443 AGATCC [GA]^GATC[CT]
MluI 593 ACGCGT A^CGCGT
NdeII 444 GATC ^GATC
NspI 237 GCATGT [GA]CATG^[CT]
NspV 127 TTCGAA TT^CGAA
PstI 478 CTGCAG CTGCA^G
SapI 178 GAAGAGC GCTCTTC(1/4)
Sau3AI 444 GATC ^GATC
SbfI 477 CCTGCAGG CCTGCA^GG
SexAI 950 ACCAGGT A^CC[AT]GGT
SfuI 127 TTCGAA TT^CGAA
SmlI 394 CTCAAG C^T[CT][GA]AG
Sse8387I 477 CCTGCAGG CCTGCA^GG
StuI 380 AGGCCT AGG^CCT
StyI 434 CCAAGG C^C[AT][AT]GG
Tsp45I 897 GTCAC ^GT[GC]AC
TspRI 916 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 443 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AvaII 114 GGACC G^G[AT]CC
138 GGACC
BanII 543 GGGCCC G[GA]GC[CT]^C
786 GAGCCC
BbrPI 154 CACGTG CAC^GTG
224 CACGTG
BsaAI 154 CACGTG [CT]AC^GT[GA]
224 CACGTG
BstAPI 293 GCAAGGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
480 GCAGGCTCTGC
DdeI 364 CTGAG C^T[ACGT]AG
827 CTGAG
DsaI 197 CCGCGG C^C[GA][CT]GG
561 CCGCGG
EarI 178 GAAGAG CTCTTC(1/4)
723 GAAGAG
Ksp632I 178 GAAGAG CTCTTC(1/4)
723 GAAGAG
KspI 197 CCGCGG CCGC^GG
561 CCGCGG
PmaCI 154 CACGTG CAC^GTG
224 CACGTG
PmlI 154 CACGTG CAC^GTG
224 CACGTG
SacII 197 CCGCGG CCGC^GG
561 CCGCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AciI AcyI AspHI AvaI
BanI BbeI BbvI BcnI BglI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BsrBI BssHII
BssKI BstNI BstUI Cac8I CfoI
Cfr13I EcoRII FauI Fnu4HI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI KasI
MaeII MboII MnlI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NlaIII NlaIV PleI Sau96I ScrFI
SfaNI SfcI SfoI SmaI TaqI
TseI TthHB8I XmaCI XmaI