Restriction map for
pF1KB7628
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwNI
AocI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I AspI AvrII
BalI BbeI BbrPI BbsI BbvCI
BciVI BclI BfrI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EarI
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI HincII HindII HindIII
HpaI KasI Ksp632I MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NarI NdeI NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I Van91I
XbaI XcmI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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Acc65I 63 GGTACC G^GTACC
AcyI 177 GGCGTC G[GA]^CG[CT]C
AhdI 542 GACGTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 275 AGCGCT AGC^GCT
Asp718I 63 GGTACC G^GTACC
AspEI 542 GACGTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 226 TGCGCA TGC^GCA
BamHI 748 GGATCC G^GATCC
BcgI 314 GCAACCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 112 CTAG C^TAG
BglI 767 GCCTTCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 177 GGCGTC G[GA]^CG[CT]C
BsgI 483 GTGCAG GTGCAG(16/14)
BsiEI 425 CGGCCG CG[GA][CT]^CG
BsmAI 648 GTCTC GTCTC(1/5)
BsmBI 647 CGTCTC CGTCTC(1/5)
BspHI 473 TCATGA T^CATGA
BspMI 351 GCAGGT ACCTGC(4/8)
BsrBI 259 CCGCTC CCGCTC(-3/-3)
BsrDI 847 GCAATG GCAATG(2/0)
BsrFI 507 GCCGGC [GA]^CCGG[CT]
BstAPI 305 GCAAGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 695 CCATGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 748 GGATCC [GA]^GATC[CT]
Cfr10I 507 GCCGGC [GA]^CCGG[CT]
DraII 773 GGGGCCT [GA]G^G[ACGT]CC[CT]
EagI 425 CGGCCG C^GGCCG
Eam1105I 542 GACGTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 425 CGGCCG C^GGCCG
Eco47III 275 AGCGCT AGC^GCT
Eco52I 425 CGGCCG C^GGCCG
EcoO109I 773 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 868 ATGCAT ATGCA^T
FspI 226 TGCGCA TGC^GCA
Hin1I 177 GGCGTC G[GA]^CG[CT]C
HphI 572 TCACC GGTGA(8/7)
KpnI 63 GGTACC GGTAC^C
KspI 211 CCGCGG CCGC^GG
MaeI 112 CTAG C^TAG
MaeII 543 ACGT A^CGT
MflI 748 GGATCC [GA]^GATC[CT]
NaeI 507 GCCGGC GCC^GGC
NcoI 695 CCATGG C^CATGG
NgoMIV 507 GCCGGC G^CCGGC
NsiI 868 ATGCAT ATGCA^T
PleI 616 GACTC GAGTC(4/5)
RcaI 473 TCATGA T^CATGA
SacI 852 GAGCTC GAGCT^C
SacII 211 CCGCGG CCGC^GG
ScaI 689 AGTACT AGT^ACT
SexAI 605 ACCTGGT A^CC[AT]GGT
SmaI 58 CCCGGG CCC^GGG
Tsp509I 389 AATT ^AATT
XhoII 748 GGATCC [GA]^GATC[CT]
XmaCI 58 CCCGGG C^CCGGG
XmaI 58 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 749 GATCC GGATC(4/5)
748 GGATC
AspHI 852 GAGCTC G[AT]GC[AT]^C
1038 GAGCAC
AvaII 183 GGACC G^G[AT]CC
784 GGACC
BanII 852 GAGCTC G[GA]GC[CT]^C
1075 GAGCCC
BsiHKAI 852 GAGCTC G[AT]GC[AT]^C
1038 GAGCAC
BsmI 16 GCATTC GAATGC(1/-1)
870 GCATTC
DpnII 321 GATC ^GATC
749 GATC
EcoT14I 695 CCATGG C^C[AT][AT]GG
701 CCTTGG
MboI 321 GATC ^GATC
749 GATC
MspA1I 126 CAGCGG C[AC]G^C[GT]G
211 CCGCGG
NdeII 321 GATC ^GATC
749 GATC
NspI 284 GCATGT [GA]CATG^[CT]
585 GCATGT
PaeR7I 132 CTCGAG C^TCGAG
1011 CTCGAG
PstI 234 CTGCAG CTGCA^G
527 CTGCAG
Sau3AI 321 GATC ^GATC
749 GATC
SfaNI 867 GATGC GCATC(5/9)
934 GATGC
StyI 695 CCATGG C^C[AT][AT]GG
701 CCTTGG
TfiI 712 GATTC G^A[AT]TC
991 GATTC
Tsp45I 571 GTCAC ^GT[GC]AC
1093 GTCAC
XhoI 132 CTCGAG C^TCGAG
1011 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BanI BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DsaI EaeI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII ItaI
MaeIII MboII MnlI MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
RsaI Sau96I ScrFI SfcI SmlI
TaqI TseI TspRI TthHB8I